Rabbit anti mcl 1 antibody

Following resections of CIVO microinjected tumors, tissue was fixed, processed, stained and scanned as previously described^{31 (link)}. Rabbit anti-CC3 antibody (Cell Signaling #9661, 1:150 dilution) and rabbit anti-MCL-1 antibody (Cell Signaling #5453, 1:500 dilution) were used for IHC analysis. For immunofluorescent detection, secondary antibody conjugated to AlexaFluor647 (Jackson Immuno research, 1:600 dilution) was applied as per manufacturer’s instructions and tissues were counterstained with DAPI.

Human immortalized keratinocytes (HaCaT) were purchased from Cell Lines Service GmbH (Eppelheim, Germany). Recombinant human TNF-α was purchased from PeproTech (Rocky Hill, NJ, USA). Oxyresveratrol (OXY) was purchased from Sigma-Aldrich (Saint Louis, MO, USA). MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was purchased from Sigma-Aldrich (Saint Louis, MO, USA). Guava^® Cell Cycle Reagent was purchased from Luminex Corporate (Austin, TX, USA). Rabbit anti-phospho-AKT (Ser473) antibody, mouse anti-AKT antibody, rabbit anti-phospho-GSK3-_β (Ser9), mouse anti-GSK3-_β antibody, rabbit anti-Ki-67 antibody, rabbit anti-MCL-1 antibody, DAPI (4′, 6-diamidino-2-phenylindole, dihydrochloride), and LY294002 (a specific inhibitor of the PI3K/AKT) were purchased from Cell Signaling Technology (Boston, MA, USA). Goat anti-mouse IgG-IRDye^®800CW and goat anti-rabbit IgG-IRDye^®680RT were purchased from Li-COR Biosciences (Lincoln, NE, USA). Goat anti-rabbit conjugated with Alexa488 was purchased from Thermo Fisher Scientific (Waltham (HQ), MA, USA).