Rabbit anti gadph

Manufactured by Merck Group

Sourced in Germany, United States

Rabbit anti-GADPH is a polyclonal antibody raised in rabbits against the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. GAPDH is a widely expressed enzyme involved in the glycolysis pathway. This antibody can be used to detect and quantify GAPDH levels in various samples.

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Lab products found in correlation

Mouse anti eea1, by BD (1 mentions) Rat anti lamp 1 1d4b, by Developmental Studies Hybridoma Bank (1 mentions) Rabbit anti rab20 11616 1 ap, by Proteintech (1 mentions) Rabbit anti rab20 gtx119559, by GeneTex (1 mentions) Mouse anti β actin antibody a5316, by Merck Group (1 mentions) Rabbit anti egfr antibody, by Santa Cruz Biotechnology (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) Goat anti rabbit igg, by Abcam (1 mentions) Goat anti mouse igg, by Santa Cruz Biotechnology (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Nu6027, by Merck Group (1 mentions) Anti cbs, by Santa Cruz Biotechnology (1 mentions) 1 fluoro 2 4 dinitrobenzene, by Merck Group (1 mentions) Sulfosalicylic acid, by Merck Group (1 mentions) Monobromobimane, by Merck Group (1 mentions) Anti β actin, by Abcam (1 mentions) Diallyl trisulfide, by Cayman Chemical (1 mentions) Tpp tissue culture dishes, by Merck Group (1 mentions) Anti 3 mst, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

GADPH (20 citations) Anti-GADPH (11 citations) Rabbit anti- (2 citations)

2 protocols using rabbit anti gadph

Immunofluorescence Analysis of Subcellular Markers

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The following primary antibodies were used: mouse anti–EEA-1 (610457, BD Transduction Laboratories, San Jose, CA); rat anti–LAMP-1 (1D4B; Developmental Studies Hybridoma Bank [DSHB, Iowa City, IA]); rat anti–LAMP-2 (ABL-93; DSHB); rabbit anti-Rab20 (11616-1-AP; Proteintech, Chicago, IL); rabbit anti-Rab20 (GTX119559; GeneTex, Irvine, CA); mouse anti–β-actin antibody (A5316; Sigma-Aldrich, Steinheim, Germany), rabbit anti-GADPH (Sigma-Aldrich), and rabbit anti-EGFR antibody (1005; Santa Cruz Biotechnology, Dallas, TX). Secondary antibodies conjugated with Alexa Fluor 488, 546, or 633 for indirect immunofluorescence studies were from Molecular Probes (Invitrogen). Secondary antibodies conjugated with horseradish peroxidase were obtained from Jackson ImmunoResearch Laboratories and Promega. Unconjugated rabbit anti-rat used for immunogold labeling was from Rockland.

Pei G., Schnettger L., Bronietzki M., Repnik U., Griffiths G, & Gutierrez M.G. (2015). Interferon-γ–inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages. Molecular Biology of the Cell, 26(17), 3061-3070.

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Quantifying Redox-Sensitive Protein Modifications

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Monobromobimane (MBB), Tris (2-carboxyethyl)phosphine hydrochloride (TCEP), sulfosalicylic acid (SSA), 1-fluoro-2,4-dinitrobenzene (DNFB), TPP® tissue culture dishes, NU6027, penicillin/streptomycin, and t-BOOH were purchased from Sigma (St. Louis, MO). Fetal bovine serum, Dulbecco’s modified Eagle’s medium (DMEM) (standard liquid media) were from Invitrogen (Rockville, MD). β-cyano-L-alanine and diallyl trisulfide were purchased from the Cayman Chemical Company (Ann Arbor, MI). Antibodies used were anti-ATR-pS435 (Cell Signaling Technology Inc, Danvers, MA, catalog number 2853s), anti-total-ATR (Invitrogen, Waltham, MA USA, catalog number PA1–450), anti-CHK1-pS345 (Invitrogen, catalog number PA5–34625), anti-total-CHK1(Abcam, Cambridge, MA, catalog number ab47574), anti-3-MST (Santa Cruz Biotechnology, Santa Cruz, CA, catalog number sc-135993), anti-CSE (Santa Cruz Biotechnology, catalog number sc-101924), anti-CBS (Santa Cruz Biotechnology, catalog number sc-67154), and anti-Visfatin (Nampt, Bethyl Laboratories, Montgomery TX, catalog number A300–779A), rabbit anti-GADPH (Sigma, catalog number G9545), and anti-beta-actin (abcam, catalog number ab8227). Secondary antibodies were goat anti-rabbit IgG (catalog numbers ab6721 & ab2040, Abcam), goat anti-mouse IgG (catalog number ab205719, Abcam), and goat anti-mouse IgG (catalog number sc358920, Santa Cruz).

Chen J., Shen X., Pardue S., Meram A.T., Rajendran S., Ghali G.E., Kevil C.G, & Shackelford R.E. (2018). The Ataxia telangiectasia-mutated and Rad3-related Protein Kinase Regulates Cellular Hydrogen Sulfide Concentrations. DNA repair, 73, 55-63.

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