Anti rabbitigg h l alexa fluor 555 conjugate

Manufactured by Cell Signaling Technology

Sourced in United States

The Anti-rabbit IgG (H + L) Alexa Fluor 555 conjugate is a secondary antibody that binds to the heavy (H) and light (L) chains of rabbit immunoglobulin G (IgG) and is conjugated to the Alexa Fluor 555 fluorescent dye. This product is suitable for use in various immunodetection techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, where it can be used to visualize and detect the presence of rabbit primary antibodies.

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Lab products found in correlation

Ab175237, by Abcam (1 mentions) V 5507, by Merck Group (1 mentions) Ab31631, by Abcam (1 mentions) Goat anti mouse igg hrp, by Huabio (1 mentions) Goat anti rabbit igg hrp, by Huabio (1 mentions) Sc 33641, by Santa Cruz Biotechnology (1 mentions) Anti insulin antibody, by Novus Biologicals (1 mentions) Tcs sp8 sted 3x, by Leica (1 mentions) Anti γh2ax, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Alexa Fluor 555 (60 citations) Alexa 555 (6 citations)

4 protocols using anti rabbitigg h l alexa fluor 555 conjugate

Multimarker Immunohistochemistry Protocol

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The primary antibodies employed were insulin in a dilution of 1:200 (mouse monoclonal,
Novus Biologicals, Littleton, CO, USA), GCG in a dilution of 1:400 (rabbit monoclonal
anti-GCG; Cell Signaling Technology), polyclonal rabbit anti-human SST in a dilution of
1:300 (Novus Biologicals), human C-peptide in a dilution of 1:100 (rabbit polyclonal;
Cell Signaling Technology), CD3 and CD20 (Genemed Biotechnologies, San Francisco, CA,
USA).
The secondary antibodies employed were anti-mouse immunoglobulin G (IgG; H + L) Alexa
Fluor 488 conjugate in a dilution of 1:200 (Cell Signaling Technology) and anti-rabbit
IgG (H + L) Alexa Fluor 555 conjugate in a dilution of 1:100 (Cell Signaling
Technology). Universal Kit Power-Stain Version 1.0 Poly Horseradish peroxidase (HRP)
3,3-diaminobenzidine (DAB) for mouse and rabbit (Genemed Biotechnologies) was employed
as a secondary antibody for CD3 and CD20.

Gabr M.M., Zakaria M.M., Refaie A.F., Ismail A.M., Khater S.M., Ashamallah S.A., Azzam M.M, & Ghoneim M.A. (2018). Insulin-producing Cells from Adult Human Bone Marrow Mesenchymal Stromal Cells Could Control Chemically Induced Diabetes in Dogs: A Preliminary Study. Cell Transplantation, 27(6), 937-947.

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Innate Immune Response Signaling Pathway

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Anti-CPSF5 (1:5000; 10322-1-AP, Proteintech), anti-CPSF6 (1:5000; ab175237, Abcam), anti-CPSF7 (1:2000; 55195-1-AP, Proteintech), anti-RIG-I (1:1000; D14G6, Cell Signaling), anti-MDA5 (1:1000; D74E4, Cell Signaling), anti-IFIT3 (1:2000; 15201-1-AP, Proteintech), anti-ISG15 (1:2000; 15981-1-AP, Proteintech), anti-DDX21 (1:2000; 10528-1-AP, Proteintech), anti-DHX15 (1:2000; 12265-1-AP, Proteintech), anti-CCL2 (1:2000; 66272-1-Ig, Proteintech), anti-IRF3 (1:1,000; sc-33641, Santa Cruz), anti-phosphorylated IRF3 (1:1000; 4D4G, Cell Signaling), anti-TBK1 (1:1000; D1B4, Cell Signaling), anti-phosphorylated TBK1 (1:1000; D52C2, Cell Signaling), anti-VSVG (1:1000; V5507, Sigma), anti-ICP27 (1:1000; ab31631, Abcam), anti-GAPDH (1:20000; 60004-1-Ig, Proteintech), anti-HA (1:5000; clone HA-7, H6533, Sigma), anti-Flag (1:5000; clone M2, F1804, Sigma), Anti-rabbit IgG (H+L), (Alexa Fluor 555 Conjugate) (1:1000, 4413, Cell Signaling), goat anti-mouse IgG-HRP (1:10000; HA1006, HuaBio), goat anti-rabbit IgG-HRP (1:10000; HA1001, HuaBio). The poly(I:C) (LMW) and poly(dA:dT) were purchased from InvivoGen. Lipofectamine 3000 and Lipofectamine RNAiMAX were purchased from Invitrogen. Act D (actinomycin D) was purchased from Sigma.

Ge Y., Huang J., Chen R., Fu Y., Ling T., Ou X., Rong X., Cheng Y., Lin Y., Zhou F., Lu C., Yuan S, & Xu A. (2024). Downregulation of CPSF6 leads to global mRNA 3’ UTR shortening and enhanced antiviral immune responses. PLOS Pathogens, 20(2), e1012061.

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Insulin and C-Peptide Antibody Staining

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Anti-insulin Antibody (1:200) (mouse monoclonal, Novus Biologicals, Littleton, CO, USA), anti-c-peptide antibody (1:100) (rabbit polyclonal; Cell Signaling Technology) were used as the primary antibodies. Anti-mouse immunoglobulin G (IgG; H ‏+ L) Alexa Fluor 488 conjugate (1:200) and anti-rabbit (IgG; H +‏ L) Alexa Fluor 555 conjugate (1:100) (Cell Signaling Technology) were utilized as the secondary antibodies.

El-Halawani S.M., Gabr M.M., El-Far M., Zakaria M.M., Khater S.M., Refaie A.F, & Ghoneim M.A. (2020). Subcutaneous transplantation of bone marrow derived stem cells in macroencapsulation device for treating diabetic rats; clinically transplantable site. Heliyon, 6(5), e03914.

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Immunofluorescent Detection of DNA Damage Markers

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Cells (1×10⁴) were affixed to frosted X slides and fixed with 4% paraformaldehyde solution for 15 min at room temperature, followed by permeabilization with 0.2% Triton X-100 for 10 min at room temperature. The slides were blocked with 5% BSA and 10% horse serum in PBS at room temperature for 1 h and were incubated with anti-γH2AX (1:200; product no. 9718; Cell Signaling Technology, Inc.) at 4°C overnight. After three rinses with PBS, the cells were incubated with anti-rabbit IgG (H+L) Alexa Fluor^® 555 Conjugate (1:500; product no. 4413; Cell Signaling Technology, Inc.) for 1 h. Then, the cells were washed twice and stained with 1 µg/ml DAPI for 10 min at room temperature. Images were acquired with a confocal microscope (Leica TCS SP8 STED 3X; Leica Microsystems, Inc.) at a magnification of ×40 and processed with ImageJ v.1.8.0 software (National Institutes of Health).

Zhang P., Wang Y., Qin M., Li D., Odhiambo W.O., Yuan M., Lv Z., Liu C., Ma Y., Dong Y, & Ji Y. (2020). Involvement of Blnk and Foxo1 in tumor suppression in BCR-ABL1-transformed pro-B cells. Oncology Reports, 45(2), 693-705.

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