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Magpix

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The MAGPIX is a multiplex detection system that uses magnetic beads and flow cytometry to simultaneously analyze multiple analytes in a single sample. The core function of the MAGPIX is to quantify multiple biomolecules, such as proteins or nucleic acids, in a multiplexed format.

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Lab products found in correlation

Gsk 3β, by Thermo Fisher Scientific (4 mentions) Pt246 pras40, by Thermo Fisher Scientific (4 mentions) Ps9 gsk 3β, by Thermo Fisher Scientific (4 mentions) Bio plex manager software, by Bio-Rad (3 mentions) Bio plex pro human cytokine 27 plex assay, by Bio-Rad (3 mentions) Human gut hormone 10 plex assay, by Bio-Rad (2 mentions) Multiplexed bead assay, by Bio-Rad (2 mentions) Bio plex 200, by Bio-Rad (2 mentions) Bio plex manager software version 6, by Bio-Rad (1 mentions) U 2900, by Hitachi (1 mentions) Milliplex human adipokine magnetic bead panel 1, by Merck Group (1 mentions) Human adipokine magnetic bead panel 2, by Merck Group (1 mentions) Bio plex manager mp software, by Bio-Rad (1 mentions) Bio plex pro assay kit, by Bio-Rad (1 mentions) Multiskan go spectrophotometer, by Thermo Fisher Scientific (1 mentions) Milliplex map, by Merck Group (1 mentions) Magplex beads, by Bio-Rad (1 mentions) Bio plex data pro software, by Bio-Rad (1 mentions) Mcytomag 70k, by Merck Group (1 mentions) Standard curves of known concentrations of recombinant human cytokines, by R&D Systems (1 mentions)

Spelling variants of this product

MAGPIX multiplex reader (37 citations) Luminex Magpix Multiplex Assay system (9 citations) Bio-Plex MAGPIX reader (9 citations) MagPix reader (8 citations) MAGPIX multiplex instrument (5 citations) Bio-Plex Magpix apparatus (4 citations) MAGPIX®200 Instrument (4 citations) Magpix assay system (3 citations) Bio-Plex MAGPIX platform (2 citations) MAGPIX multiplex analyzer (2 citations)

42 protocols using magpix

1

Multiplex Hormone Assay for Gut Dysregulation

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For these studies, we used a Human Gut Hormone 10-Plex ™ Assay (Bio-Rad, Hercules, CA), which is based on a magnetic bead-based format for simultaneously measuring immunoreactivity to insulin, C-peptide, gastric inhibitory polypeptide (GIP), glucagon-like peptide-1 (GLP-1), leptin, ghrelin, glucagon, resistin, plasminogen activator inhibitor-1 (PAI-1), and visfatin in tissue homogenates. This assay was utilized to investigate a fuller spectrum of potential polypeptide abnormalities that could contribute to impairments in energy balance within the CNS (Table 1). The assays were performed in accordance with the manufacturer’s protocol. In brief, captured antigens were detected with biotinylated secondary antibodies followed by a streptavidin-phycoery-thrin reporter conjugate. Fluorescence intensity was measured in a MAGPIX (Bio-Rad, Hercules, CA) and hormone concentrations (pg/mL) were determined from standard curves using MAGPIX software.
Robbins J., Busquets O., Tong M, & de la Monte S.M. (2020). Dysregulation of Insulin-Linked Metabolic Pathways in Alzheimer’s Disease: Co-Factor Role of Apolipoprotein E ɛ4. Journal of Alzheimer's Disease Reports, 4(1), 479-493.
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2

Quantifying Plasma Leptin and Ghrelin

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Thawed plasma samples were diluted 1:4 in Universal Assay Buffer (UAB). Blood concentrations of leptin and total ghrelin (acylated + de-acylated) were measured in duplicate using Bio-Plex Pro Human Diabetes Assays (#171A7001M, Bio-Rad, Hercules, CA) according to the manufacturer’s protocol and specifications. The Bio-Plex Pro Assays are immunoassays on magnetic beads that utilize principles similar to those of a sandwich ELISA, with capture antibodies against leptin and ghrelin covalently coupled to beads. Briefly, captured antigens against leptin and ghrelin were detected with biotinylated secondary antibodies followed by a streptavidin-phycoerythrin (SA-PE) reporter conjugate, where PE serves as the fluorescent reporter. Fluorescence intensity was measured in a MAGPIX (Bio-Rad, Hercules, CA) and hormone concentrations (pg/mL) were determined from standard curves with MAGPIX software.
According to the manufacturer specifications, for leptin, the lower limit of quantification is 3 pg/mL and the upper limit is 41,614 pg/mL. The intra-assay coefficient of variation is 4% and the inter-assay coefficient of variation is 4%. For ghrelin, the lower limit of quantification is 3 pg/mL and the upper limit is 41,664 pg/mL. The intra-assay coefficient of variation is 4% and the inter-assay coefficient of variation is 3% (2019 ).
Daniels T.E., Mathis K.J., Gobin A.P., Lewis-de los Angeles W.W., Smith E.M., Chanthrakumar P., de la Monte S, & Tyrka A.R. (2022). Associations of Early Life Stress with Leptin and Ghrelin in Healthy Young Adults. Psychoneuroendocrinology, 149, 106007.
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3

Multiplex Assay for Gut Hormones

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The Human Gut Hormone 10-Plex ™ Assay (Bio-Rad, Hercules, CA), a magnetic bead-based multiplex ELISA, was used to measure serum and CSF immunoreactivity to insulin, leptin, ghrelin, gastric inhibitory polypeptide (GIP), glucagon like peptide-1 (GLP-1), pancreatic polypeptide (PP), and pancreatic peptide YY (PYY). The polypeptides measured help maintain energy balance both systemically and in the CNS (Table 1). The assays were performed in accordance with the manufacturer’s protocol. In brief, duplicate serum (diluted 1:4 in assay dilution buffer) and CSF (undiluted) samples were incubated with magnetic beads covalently coupled with capture antibodies. Captured antigens were detected with biotinylated secondary antibodies followed by a streptavidin-phycoerythrin reporter conjugate. Fluorescence intensity was measured in a MAGPIX (Bio-Rad, Hercules, CA) and hormone concentrations (pg/mL) were determined from standard curves using MAGPIX software.
Fujiwara T., Tanaka K., Inoue E., Kikyo M, & Takai Y. (1999). Bni1p Regulates Microtubule-Dependent Nuclear Migration through the Actin Cytoskeleton in Saccharomyces cerevisiae. Molecular and Cellular Biology, 19(12), 8016-8027.
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4

Serum Cytokine Profiling in Mice

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Determining the serum cytokine levels was performed using the 23-plex-mouse cytokine group I Bioplex kit (BioRad, Hercules, CA) per manufacturer’s instruction. In brief, sera samples were diluted 1:25,000 in the provided assay buffer. A 96-well assay plate was prewet with 100 μl of assay buffer. Pre-mixed beads were added to each well in a 50 μl volume and then washed, using a magnet for bead retention, before adding 50 μl of diluted sample, standard, or blank. The assay plate was incubated for 30 minutes by shaking at room temperature in the dark. The plate was then washed prior to the addition of detection antibody. Following the detection antibody the plate was washed by adding 50 μl of Streptavidin-PE per well. The samples were in incubated for 10 minutes by shaking. The plate was washed for the final time and 125 μl of assay buffer was added. The results were acquired using BioRad Magpix. The data were analyzed using the Bio-Plex Manager software (BioRad, Hercules, CA).
Voigt A., Esfandiary L., Wanchoo A., Glenton P., Donate A., Craft W.F., Craft S.L, & Nguyen C.Q. (2016). Sexual dimorphic function of IL-17 in salivary gland dysfunction of the C57BL/6.NOD-Aec1Aec2 model of Sjögren’s syndrome. Scientific Reports, 6, 38717.
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5

Multiplex Cytokine Profiling in Vitreous Humor

Cited 1 time
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The concentrations of 27 cytokines, basic fibroblast growth factor (FGF), eotaxin, G-CSF (granulocyte colony-stimulating factor), GM-CSF (granulocyte-macrophage colony-stimulating factor), interferon-γ (IFN-γ), interleukin (IL)-1β, IL-1 receptor antagonist (IL-1ra), IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, IFN Inducible Protein 10 (IP-10), monocyte chemotactic protein-1 (MCP-1), macrophage inflammatory protein-1α (MIP-1α), MIP-1β, platelet derived growth factor BB (PDGF-BB), regulated on activation, normal T-cell expressed and secreted (RANTES), tumor necrosis factor-α (TNF-α), vascular endothelial growth factor (VEGF), and vitreous humor samples were measured using a multiplex assay instrument (MAGPIX; Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s instructions and previous report by Fukunaga and colleagues [61 (link)]. Levels of vitreous humor cytokines below detectable levels were given as 0 for statistical analysis.
Ando Y., Keino H., Inoue M., Hirota K., Takahashi H., Sano K., Koto T., Sato T., Takeuchi M, & Hirakata A. (2022). Circulating Vitreous microRNA as Possible Biomarker in High Myopic Eyes with Macular Hole. International Journal of Molecular Sciences, 23(7), 3647.
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6

Comprehensive Proteomic Profiling of Vitreous Mediators

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Multiplex proteomic analysis was used to measure the levels of inflammatory cytokines, chemokines, and growth factors in the vitreous samples. The concentrations of 33 human mediators (IL-1β, IL-1 receptor antagonist, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17A, IL-22, IL-26, IL-27p28, IFN-α2, IFN-β, IFN-γ, TNF-α, eotaxin, MCP-1, MIP-1α, MIP-1β, RANTES, IP-10, G-CSF, granulocyte-macrophage-CSF, bFGF, PDGF-BB, and VEGF; Table 3) were measured using a multiplex assay instrument (MAGPIX; Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s instructions. There were no samples in this study where concentrations were above the upper range that could be analysed.
Fukunaga H., Kaburaki T., Shirahama S., Tanaka R., Murata H., Sato T., Takeuchi M., Tozawa H., Urade Y., Katsura M., Kobayashi M., Wada Y., Soga H., Kawashima H., Kohro T, & Aihara M. (2020). Analysis of inflammatory mediators in the vitreous humor of eyes with pan-uveitis according to aetiological classification. Scientific Reports, 10, 2783.
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7

Multiplex ELISA Profiling of Insulin Signaling

Cited 1 time
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We used bead-based multiplex ELISAs to measure immunoreactivity to the
insulin receptor (IR), IGF-1 receptor (IGF-1R), IRS-1, Akt, proline-rich Akt
substrate of 40 kDa (PRAS40), ribosomal protein S6 kinase (p70S6K), and glycogen
synthase kinase 3β (GSK-3β), and pYpY1162/1163-IR,
pYpY1135/1136-IGF-1R, pS312-IRS-1,
pS473-Akt, pT246-PRAS40, pTpS421/424-p70S6K,
and pS9-GSK-3β (Invitrogen, Carlsbad, CA). Samples (100
μg protein) were incubated with the beads, and captured antigens were
detected with secondary antibodies and phycoerythrin-conjugated anti-rabbit IgG
[33 (link)]. Plates were
read in a MAGPIX (Bio-Rad, Hercules, CA).
Andreani T., Tong M., Gundogan F., Silbermann E, & de la Monte S.M. (2016). Differential Effects of 3rd Trimester-Equivalent Binge Ethanol and Tobacco-Specific Nitrosamine Ketone Exposures on Brain Insulin Signaling in Adolescence. Journal of diabetes and related disorders, 1(1), 105.
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8

Serum Cytokine Profiling via Multiplex Assay

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The serum cytokine levels were determined using the 8-plex-mouse cytokine group I Bioplex kit (BioRad, Hercules, CA) per the manufacturer’s instruction. In brief, sera samples were diluted 1:4 in the provided assay buffer. A 96-well assay plate was prewet with 100 μl of assay buffer. Pre-mixed beads were added to each well in a 50 μl volume and then washed, using a magnet for bead retention, before adding 50 μl of the diluted sample, standard, or blank. The assay plate was incubated for 30 min by shaking at room temperature in the dark. The plate was then washed before the addition of the detection antibody. Following the detection antibody, the plate was washed by adding 50 μl of Streptavidin-PE per well. The samples were incubated for 10 min by shaking. The plate was washed for the final time, and 125 μl of assay buffer was added. The results were acquired using BioRad Magpix. The data were analyzed using the Bio-Plex Manager software (BioRad, Hercules, CA).
Gupta S., Li D., Ostrov D.A, & Nguyen C.Q. (2021). Blocking I—Ag7 class II major histocompatibility complex by drug-like small molecules alleviated Sjögren’s syndrome in NOD mice. Life sciences, 288, 120182.
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9

Cytokine Profiling of CAR T Cells

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Cytokines produced by CAR T cells after stimulation with anti-DR or anti-CD3/CD28 antibodies, were measured using a multiplexed bead assay (Bio-Rad) and analyzed using a Bio-Rad MagPix. 1 × 106 CAR T cells were cultured in a 48 well plate in which the wells had been coated with 250 μl of an anti-DR antibody (LB 3.1, 2 μg/ml), or anti-CD3 and anti-CD28 antibodies (1 μg/ml each), or PBS. After 18 hr of stimulation, 500 ul of supernatant was collected and frozen @ −20° C prior to the cytokine ELISA. Supernatants were assayed for expression of IFNγ, TNFα, IL-10, IL-17A, and IL-6. Standard curves were used to calculate cytokine concentration, and data are based on duplicate samples and representative of two independent experiments.
Whittington K.B., Prislovsky A., Beaty J., Albritton L., Radic M, & Rosloniec E.F. (2021). CD8+ T cells expressing an HLA-DR1 chimeric antigen receptor (CAR) target autoimmune CD4+ T cells in an antigen specific manner and inhibit the development of autoimmune arthritis. Journal of immunology (Baltimore, Md. : 1950), 208(1), 16-26.
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10

Multiplex ELISA Assay for Insulin Signaling

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We used bead-based multiplex ELISAs to measure immunoreactivity to the insulin receptor (IR), IGF-1 receptor (IGF-1R), IRS-1, Akt, proline-rich Akt substrate of 40 kDa (PRAS40), ribosomal protein S6 kinase (p70S6K), and glycogen synthase kinase 3β (GSK-3β), and pYpY1162/1163-IR, pYpY1135/1136-IGF-1R, pS312-IRS-1, pS473-Akt, pT246-PRAS40, pTpS421/424-p70S6K, and pS9-GSK-3β (Invitrogen, Carlsbad, CA). Samples (50 μg protein) were incubated with the beads, and captured antigens were detected with secondary antibodies and phycoerythrin-conjugated anti-rabbit IgG. Plates were read in a MAGPIX (Bio-Rad, Hercules, CA).
de la Monte S.M., Tong M., Agarwal A, & Cadenas E. (2016). Tobacco Smoke-Induced Hepatic Injury with Steatosis, Inflammation, and Impairments in Insulin and Insulin-Like Growth Factor Signaling. Journal of clinical & experimental pathology, 6(2), 269.
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