Anti trpv4 primary antibody

Anti‐α‐SMA, GSK2193874 (GSK219), GSK1016790A (GSK101), A23187 (A23; calcium ionophore) were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Anti‐TRPV4 primary antibody was purchased from Alomone Labs (Jerusalem, Israel), and anti‐GAPDH antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Mouse and rabbit anti‐goat IgG were purchased from Jackson Immuno‐Research (West Grove, PA, USA). Anti‐ECAD, anti‐NCAD, anti‐YAP/TAZ, anti‐YAP, anti‐TAZ, anti‐AKT, anti‐phospho‐AKT (anti‐p‐Ser473 AKT), anti‐Smad2, anti‐Smad3, anti‐p‐Smad2, and anti‐p‐Smad3 antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). TGFβ1 was purchased from R&D Systems (Minneapolis, MN, USA). Alexa Fluor 488/594 conjugated IgG and Prolong diamond DAPI were purchased from Thermo Fisher Scientific (Waltham, MA, USA). FLIPR Calcium 5 assay kit was purchased from Molecular devices (Sunnyvale, CA, USA). Easy coat polyacrylamide hydrogels of various degrees of stiffnesses (0.5, 1, 8, and 25 kPa) were purchased from Matrigen Life Technologies (Brea, CA, USA). Immunoblotting‐related reagents were purchased from Bio‐Rad Laboratories (Hercules, CA, USA). All other chemicals were purchased from Sigma or Thermo Fisher Scientific unless otherwise indicated.