Blpr2

Manufactured by World Precision Instruments

Sourced in United States

The BLPR2 is a laboratory equipment designed for precise pressure and flow measurements. It features a high-accuracy pressure sensor and integrated digital display to provide real-time readings. The device is suitable for a range of applications that require accurate pressure monitoring or control.

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Lab products found in correlation

Labview software, by National Instruments (2 mentions) Tropicamide, by Alcon (1 mentions) Xylazine, by Akorn (1 mentions) Ketamine, by Henry Schein (1 mentions) Three way stopcock, by Terumo (1 mentions) Lab trax 4 16, by World Precision Instruments (1 mentions) Powerlab 26t, by ADInstruments (1 mentions) Fort 10g, by World Precision Instruments (1 mentions) Phd 2000, by Harvard Apparatus (1 mentions) Digidata 1440a, by Molecular Devices (1 mentions)

8 protocols using blpr2

Anesthesia and Monitoring Protocol for Ophthalmic Imaging

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For all imaging, anesthesia was induced with intramuscular ketamine (15 mg/kg; Henry Schein Animal Health, Dublin, OH, USA) and xylazine (1.5 mg/kg; Akorn, Inc., Decatur, IL, USA), along with a single subcutaneous injection of atropine sulfate (0.05 mg/kg; Butler Schein Animal Health, Dublin, OH, USA). The animals were intubated and breathed air plus 10% oxygen spontaneously. Heart rate, end-tidal CO₂, and arterial oxygenation saturation were monitored continuously. Body temperature was maintained at 37°C using a warming blanket. For the ONH blood flow and OCT imaging procedures, pupils were fully dilated with 1.0% tropicamide (Alcon Laboratories, Inc., Fort Worth, TX, USA). One of the superficial branches of a tibial artery was cannulated with a 27-gauge needle, which was connected to a pressure transducer (BLPR2; World Precision Instruments, Sarasota, FL, USA) and a four-channel amplifier system (Lab-Trax-4/24T; World Precision Instruments) for continuous arterial blood pressure recording throughout each experiment. Anesthesia was maintained by continuous administration of pentobarbital (8–12 mg/kg/h, intravenous) using an infusion pump (Aladdin; World Science Instruments, Inc., Sarasota, FL, USA). During the ONT surgical procedure, anesthesia was maintained by 1.5% to 3% isoflurane in oxygen.

Ing E., Ivers K.M., Yang H., Gardiner S.K., Reynaud J., Cull G., Wang L, & Burgoyne C.F. (2016). Cupping in the Monkey Optic Nerve Transection Model Consists of Prelaminar Tissue Thinning in the Absence of Posterior Laminar Deformation. Investigative Ophthalmology & Visual Science, 57(6), 2914-2927.

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Cardiovascular Monitoring Protocol

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Mean artery BP was monitored with a transducer, model BLPR2 (World Precision Instruments, Sarasota, FL, United States) connected to an ad-hoc bridge amplifier (home-made) and recorded using LabView software (National Instruments S.R.L., Milan, Italy). The instrument measured BP every 60 s and automatically provided an averaged value every 5 min. ECG recording (at a sampling rate of 1 KHz) was performed with a home-made differential amplifier for biomedical signals and data acquisition was obtained by LabView software.
The signal of HR was analyzed beat-to-beat to quantify changes and BP values recorded every 5 min were stored in a computer for off-line analyses. For statistical analysis and for graphical representations, we used values at intervals of 10 and 20 min. We considered as baseline value, the average of three recordings obtained at 10 min intervals immediately prior to ME1, then we considered recordings at 10 min interval for 30 min (corresponding to ME1, POST ME1 and ME2 periods) and at 20 min interval for the subsequent observation period.

Sabatino L., Costagli C., Lapi D., Del Seppia C., Federighi G., Balzan S., Colantuoni A., Iervasi G, & Scuri R. (2018). Renin-Angiotensin System Responds to Prolonged Hypotensive Effect Induced by Mandibular Extension in Spontaneously Hypertensive Rats. Frontiers in Physiology, 9, 1613.

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Measuring Intranodal Pressure in Mice

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The mouse was anesthetized and an arc‐shaped incision made in the abdominal skin from the SiLN to the PALN (Fig. 1B). As indocyanine green (ICG) injected into the SiLN seldom flowed across the midline to the contralateral side and always flowed from the SiLN to the PALN, it is unlikely that surgery damaged the lymphatic vessels connecting the SiLN and PALN or influenced INP measurements. A 21‐gauge hypodermic needle was connected to a pressure transducer (BLPR2; World Precision Instruments, Sarasota, FL, USA) through a three‐way stopcock (Terumo, Tokyo, Japan) filled with physiological saline. The pressure transducer was connected to a directly coupled amplifier system (Bridge8; World Precision Instruments) linked to a computer running analysis software (LabScribe2; iWorx Systems, Dover, NH, USA). A zero reading was obtained with the needle open to the air at the level of the node to be measured.19 Intranodal pressure was measured (0.02 s sampling rate) with the needle inserted for 5 min into the central region of the LN.

Miura Y., Mikada M., Ouchi T., Horie S., Takeda K., Yamaki T., Sakamoto M., Mori S, & Kodama T. (2016). Early diagnosis of lymph node metastasis: Importance of intranodal pressures. Cancer Science, 107(3), 224-232.

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Cardiovascular Monitoring in Animal Experiments

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Throughout all experiments MABP and HR were continuously recorded. MABP was monitored with a blood pressure transducer BLPR2 (World Precision Instruments, Sarasota, FL, USA) connected to an ad hoc bridge amplifier (home-made) and recorded using a LabView software (National Instruments S.R.L., Milan, Italy). ECG recording (at a sampling rate of 1 KHz) was done with a home-made differential amplifier for biomedical signals and data acquisition was obtained by a LabView software. HR was calculated from the R-R interval. Data were recorded and stored in a computer for off-line analyses.
The average of three measurements obtained every 10 min over the 30 min immediately prior to ME1 (baseline observation) represented the baseline value. Post-treatment measurements were obtained immediately after ME and every 5 min for the whole observation period. For a better graphical representation, in the graphs data were plotted every 20 min.

Lapi D., Varanini M., Colantuoni A., Del Seppia C., Ghione S., Fommei E, & Scuri R. (2017). Repeated Mandibular Extension in Rat: A Procedure to Modulate the Cerebral Arteriolar Tone. Frontiers in Physiology, 8, 625.

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Aqueous Outflow Facility Measurement

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Aqueous outflow facility (C) was measured in 7-week Dex-treated mice by constant flow infusion as previously described^{67 (link),68 (link)}. Briefly, animals were anesthetized (ketamine 100 mg/kg; xylazine 10 mg/kg, I.P.), and the tip of a 30G steel needle was carefully placed into the anterior chamber. The needle was connected by tubing to a flow-through pressure transducer (BLPR2, World Precision Instruments (WPI), Sarasota, FL USA). The opposing end of the transducer was connected to a 1mL syringe filled with sterile filtered PBS loaded into an infusion pump (Microdialysis SP101i, WPI). A virtual chart recorder (LabScribe2, WPI) continuously displayed pressure signals. Following equilibration (20–30 min), eyes were infused at a flow rate of 100 nL/min. When pressure had stabilized (10–15 min), flow rates were increased sequentially (200 to 500 nL/min, in 100 nL/min increments). Three stabilized pressures at each flow rate were recorded, and the average value calculated as mean stabilized pressure. C was calculated as the reciprocal of the slope of a plot of Mean Stabilized Pressure as the ordinate and Flow Rate the as abscissa.

Kasetti R.B., Maddineni P., Millar J.C., Clark A.F, & Zode G.S. (2017). Increased synthesis and deposition of extracellular matrix proteins leads to endoplasmic reticulum stress in the trabecular meshwork. Scientific Reports, 7, 14951.

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Evaluation of Automated Glaucoma Valve Devices

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Seventeen AGV devices (model FP7; New World Medical, Rancho Cucamonga, CA) were tested in this study. The AGV specifications are shown in Figure 1. Physiologic saline (0.7% sodium chloride), disposable 27-G needles (NN-2719S), and 5-mL syringes (SS-05Sz) were purchased from Terumo Corporation (Tokyo, Japan). Infusion tubes (JV-NDH1050FL and JV-ND1010PC) were purchased from JMS Co., Ltd. (Tokyo, Japan). An infusion syringe pump (SP101i) was purchased from Kd Scientific (Holliston, MA). A pressure transducer (BLPR2), 4-channel transducer amplifier (SYS-TBM4M), analog-to-digital converter (LAB-TRAX-4/16), and pressure curve analysis software LabScribe2 (LAB-TRAX-4) were purchased from World Precision Instrument (Sarasota, FL).

Masdipa A., Kaidzu S, & Tanito M. (2023). Flow Pressure Characteristics of the Ahmed Glaucoma Valve and Possible Effect of Entrapped Air in the Tube. Translational Vision Science & Technology, 12(4), 16.

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Isolated Rabbit Heart Preparation for Imaging

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New Zealand white rabbits (n = 11, ~ 3 kg) were used in the study according to a protocol approved by the Committee on the Use and Care of Animals at University of Michigan (Protocol #: PRO00003842). The animals were anesthetized intramuscularly via ketamine (35 mg/kg) and xylazine (5 mg/kg), heparinized (1000 U/kg), and euthanized intravenously by injection of sodium pentobarbital (100 mg/kg). The heart was harvested after mid-sternotomy, and rapidly washed with warmed Tyrode’s solution. As described before (Laughner et al. 2012b (link)), the heart was immediately placed on a Langendorff apparatus and retrogradely perfused with oxygenated Tyrode’s solution (pH = 7.35 ± 0.05; 37 °C; 95% O₂/5% CO₂) via aorta at constant pressure (60 – 80 mmHg). Peripheral tissue was cleaned and the heart was superfused in Tyrode’s solution at 35 – 37 °C. Excitation-contraction decoupler, 2, 3-butanedione monoxime (BDM, 15 mM; Fisher Scientific) was administered to mechanically silence the heart beat motion that can introduce artifacts during imaging. The electrocardiogram was recorded with two needle electrodes and digitized (Powerlab 26T, ADInstruments) while the aortic perfusion pressure was continuously monitored (BLPR2, World Precision Instruments) to ensure physiological stability of the heart preparation.

Wu Z., Kumon R.E., Laughner J.I., Efimov I.R, & Deng C.X. (2014). Electrophysiological Changes Correlated with Temperature Increases Induced by High-Intensity Focused Ultrasound Ablation. Ultrasound in medicine & biology, 41(2), 432-448.

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Bladder Pressure and Voiding Measurement

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The mice were anesthetized with urethane (1.3 g/kg, S.C.) and their anesthesia state was confirmed through a gentle toe pinch. Subsequently, a laparotomy was meticulously performed to expose the bladder, following the same procedure used for viral vector injection. Then, a polyethylene catheter (KN-392, Natsume Seisakusho Co. Ltd, Tokyo, Japan) connected to a pressure sensor (BLPR2, World Precision Instruments, LLC, Sarasota, FL, USA) and a syringe pump (PHD 2000, Harvard Apparatus, Holliston, MA, USA) was inserted into the dome region of the bladder. After 2 h of recovery time post-catheterization, PBS or PBS diluted with 0.1% Acetic acid was infused into the bladder at a rate of 3 mL/h. To measure the voiding volume of the mice, a force transducer (FORT10G, World Precision Instruments, LLC, Sarasota, FL, USA) with a plastic cup was employed. Real-time data collection of intravesical pressure of the bladder and the voiding volume was conducted at a sampling rate of 500 Hz using a data acquisition system (Digidata 1440a, Molecular Devices, San Jose, CA, USA).

Hong J.K., Moon H.J, & Shin H.J. (2023). Optical EUS Activation to Relax Sensitized Micturition Response. Life, 13(10), 1961.

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