Culture insert 2 well in

Manufactured by Ibidi

The Culture-Insert 2 Well is a laboratory equipment designed for cell culture. It provides a platform for growing and maintaining cells in a controlled environment. The product consists of two individual wells, allowing for the simultaneous cultivation of different cell types or experimental conditions.

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Lab products found in correlation

μ dish 35 mm, by Ibidi (2 mentions) Nis element software, by Nikon (1 mentions) Ds fi3 camera, by Nikon (1 mentions) Eclipse te2000 u microscope, by Nikon (1 mentions) Incucyte s3 live cell analysis system, by Sartorius (1 mentions)

2 protocols using culture insert 2 well in

Migration Assays for IPFP-MSCs

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IPFP-MSCs were seeded into Culture-Insert 2 Well in μ-Dish ^35mm (Ibidi) or onto cartilage surface, and cultured for 24 h. Culture-Insert was removed and scratches on cartilage were made in the plate using a P200 pipette tip. After washing with PBS three times, the culture medium was changed with DMEM/F12 supplemented with 0.9% NS or HA or PRP or TE (mixing ratio: 1:1). To analyze the migration of IPFP-MSCs, images were taken at 0, 24, and 48 h, and cells that crossed the baseline were counted for analysis.
Besides, 5 × 10⁴ cells/well of IPFP-MSCs were seeded into the upper chamber of Transwell (Corning, 8 μm) filled with DMEM/F12. The lower chamber was filled with DMEM/F12 supplemented with 0.9% NS or HA or PRP or TE (mixing ratio: 1:1). After 24 h, cells in the upper surface of the membrane were removed with a cotton swab. Cells that migrated to the other side of the membrane were fixed with 4% paraformaldehyde and stained with crystal violet. These cells were imaged and counted in 6 random view fields.

Yang J., Wang X., Fan Y., Song X., Wu J., Fu Z., Li T., Huang Y., Tang Z., Meng S., Liu N., Chen J., Liu P., Yang L., Gong X, & Chen C. (2021). Tropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad MSCs and reduces osteoarthritis progression. Bioactive Materials, 10, 443-459.

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Monitoring Cell Migration and Invasion Dynamics

Cited 1 time

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In wound-healing assay, 7.5X10⁴ cells were seeded in Culture-Insert 2 well in μ-Dish 35 mm (Ibidi) and allowed to form monolayer. The insert was then removed and the migration area in the wound was imaged and evaluated in an IncuCyte S3 Live-Cell Analysis System (Sartorius) constantly for up to 3 days. Invasion assay was performed as previously published (16 (link)). Cells (2-2.5X10⁴ for KPL4 and 3.5X10⁴ for FC-IBC02) were plated in 6.5 mm Transwell champers (Sigma). 6 to 18 h later, depending on the invasive potential of the cell line, the cells that had invaded through the filter and attached to its bottom surface were stained with crystal-violet, imaged and counted in five independent fields/well using a Nikon Eclipse TE2000-U microscope equipped with a DS-FI3 Camera and NIS-Element Software (Nikon).

Thomas C., Karagounis I.V., Srivastava R.K., Vrettos N., Nikolos F., Francois N., Huang M., Gong S., Long Q., Kumar S., Koumenis C., Krishnamurthy S., Ueno N.T., Chakrabarti R, & Maity A. (2021). Estrogen receptor β-mediated inhibition of actin-based cell migration suppresses metastasis of inflammatory breast cancer. Cancer research, 81(9), 2399-2414.

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