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Phosphorylated h2ax γh2ax

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Phosphorylated H2Ax (γH2AX) is a histone protein that is phosphorylated in response to DNA double-strand breaks. It serves as a marker for the identification and quantification of DNA damage.

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Lab products found in correlation

Brca1, by Merck Group (2 mentions) Fancd2, by Novus Biologicals (2 mentions) Rad51, by Novus Biologicals (2 mentions) Apobec3b, by Novus Biologicals (1 mentions)

2 protocols using phosphorylated h2ax γh2ax

1

Isolation and Characterization of HPV-Positive Keratinocytes

Cited 1 time
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Healthy human foreskin keratinocytes (HFKs) were isolated from deidentified neonatal specimens. HFK cells were transfected with HPV16 or HPV31 genomes to generate HFK-16 and HFK-31 cell lines that contain episomal HPV DNA as previously described (18 (link)). CIN 612 cells were isolated from a cervical cancer biopsy specimen and contain HPV31 episomes (19 (link)). All keratinocytes were cocultured in E-media with growth-arrested NIH 3T3 fibroblasts as described previously (3 (link)). Antibodies to phosphorylated CHK1 (pCHK1) (catalog no. 12302; Cell Signaling Technology [CST]), phosphorylated CHK2 (pCHK2) (catalog no. 2661; CST), FANCD2 (catalog no. 100182; Novus), phosphorylated H2Ax (γH2AX) (catalog no. 05636; Millipore), phosphorylated SMC1 (pSMC1) (catalog no. 4805S; CST), BRCA1 (catalog no. OP92; Millipore), RAD51 (catalog no. NB100148; Novus), and TOP2β (catalog no.A300-950A; Bethyl Laboratories) were used.
Kaminski P., Hong S., Kono T., Hoover P, & Laimins L. (2021). Topoisomerase 2β Induces DNA Breaks To Regulate Human Papillomavirus Replication. mBio, 12(1), e00005-21.
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2

DNA Damage Response Signaling Pathway

Cited 1 time
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p16 (#550834; BDPharmingen), phosphorylated CHK1 (pCHK1) (#12302; CST), phosphorylated CHK2 (pCHK2) (#2661; CST), FANCD2 (#100182; Novus), phosphorylated H2Ax (γH2AX) (#05636 Millipore), phosphorylated SMC1 (pSMC1) (#4805S; CST), BRCA1 (#OP92; Millipore), RAD51 (#NB100148; Novus), APOBEC3A (#HPA043237; Sigma), APOBEC3B (#NBP256411; Novus), APOBEC3B (mAb 5210-87-13) (Gift from Reuben Harris lab)) [31 (link)]
Kono T., Hoover P., Poropatich K., Paunesku T., Mittal B.B., Samant S, & Laimins L.A. (2020). Activation of DNA damage repair factors in HPV positive oropharyngeal cancers.. Virology, 547, 27-34.
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