Apc rat anti mouse cd19 clone 1d3

Manufactured by BD

Sourced in United States

The APC rat anti-mouse CD19 clone 1D3 is a flow cytometry reagent used for the identification and quantification of mouse CD19-positive cells. CD19 is a cell surface antigen expressed on B cells and a subset of plasma cells. This reagent is conjugated with Allophycocyanin (APC), a fluorescent dye, to facilitate detection and analysis of CD19-positive cells.

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Lab products found in correlation

Anti human cd11b pe, by BD (1 mentions) Facscanto 2 cytometer, by BD (1 mentions)

Close spelling variants of this product

Anti-mouse CD19-APC (clone 1D3, Anti-CD19 (clone 1D3, APC Rat Anti-Mouse CD19 Anti-mouse CD19 (1D3, CD19 (clone 1D3, Anti-CD19 (1D3, CD19 (1D3, Anti-CD19-APC Clone 1D3 CD19-APC

2 protocols using apc rat anti mouse cd19 clone 1d3

Murine Splenic Cell Enumeration

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For the determination
of splenic cell counts, mouse spleens were harvested and the cells
dispersed in media by gentle teasing and then centrifuged at 500g. Cell pellets were resuspended in media and total spleen
cell counts were performed manually using a standard hemocytometer
and light microscopy. Lymphocyte subsets were quantified by flow cytometry
using the following antibody reagents: BV605 rat anti-mouse CD3 clone
17A2 (#564009, BD Biosciences); BV786 rat anti-mouse CD4 clone RM4–5
(#563727, BD Biosciences); BV711 rat anti-mouse CD8a clone 53-6.7
(#100748, BioLegend); PE-Cy7 mouse anti-mouse NK1.1 clone PK136 mouse
(#552878, BD Biosciences); APC rat anti-mouse CD19 clone 1D3 (#550992,
BD Biosciences). Peripheral blood cell counts and spleen white blood
cell counts were determined on a Beckman Coulter Ac.T diff hematology
analyzer.

Gartlan K.H., Jaiswal J.K., Bull M.R., Akhlaghi H., Sutton V.R., Alexander K.A., Chang K., Hill G.R., Miller C.K., O’Connor P.D., Jose J., Trapani J.A., Charman S.A., Spicer J.A, & Jamieson S.M. (2022). Preclinical Activity and Pharmacokinetic/Pharmacodynamic Relationship for a Series of Novel Benzenesulfonamide Perforin Inhibitors. ACS Pharmacology & Translational Science, 5(6), 429-439.

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Comprehensive Immune Cell Profiling of Lung and Blood

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Staining of digested single cell suspensions of lung tissue and blood leukocytes was performed for 15 minutes using fluorescently labelled antibodies: PerCP rat anti-mouse CD45 (30-F11 clone, 1:100, 557,235, BD), FITC anti-mouse CD11b (M1/70 clone, 1:100, 101,206, Biolegend), PE-Cy™7 rat anti-mouse Ly6G (clone 1A8, 1:100, 560,601, BD), APC anti-mouse Ly6C (clone HK1.4, 1:100, 560,595, BD), FITC rat anti-mouse CD3 (clone 17A2, 1:100, 561,798, BD), PE-Cy™7 rat anti-mouse CD4 (clones RM4–5 clone, 1:100, 552,775, BD), APC-H7 rat anti-mouse CD8a (clone 53–6.7, 1:100, 560,182, BD), APC rat anti-mouse CD19 (clone 1D3, 1:1000, 550,992, BD), PE rat anti-mouse CD49b (clone DX5, 1:1000, 553,858, BD), PE anti-mouse F4/ 80 (BM8 clone, 1:1000,123,110, Biolegend) and Alexa Fluor 647-labelled anti-mouse Msr1 (2F8 clone, 1:1000, MCA1322A647, AbD Serotec, NC, USA). Staining was performed with PE anti-human CD11b (ICRF44 clone, 1:1000, 555,388, BD), PE-Cy7 anti-human CD66b (G10F5 clone, 1:1000, 305,116, Biolegend) and APC anti-human Msr1 (7C9C20 clone, 1:1000, 371,905, Biolegend) stained human blood. All assays were performed by FACS Canto II cytometer (BD). Cell sorting was performed using FACSAria. Data were analysed using FlowJo software (Tree Star, Inc., Ashland, OR).

Zhang H., Wu D., Wang Y., Shi Y., Shao Y., Zeng F., Spencer C.B., Ortoga L., Wu D, & Miao C. (2024). Ferritin-mediated neutrophil extracellular traps formation and cytokine storm via macrophage scavenger receptor in sepsis-associated lung injury. Cell Communication and Signaling : CCS, 22, 97.

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