Sali hindiii

The DNA regions containing yafW-ykfI, yfjZ-ypjF, five genes in CP4-6 (Supplementary Figure S1C) and five genes in CP4-57 (Supplementary Figure S1F) were amplified from BW25113 using primer pairs listed in Supplementary Table S3. The expected PCR products were digested with SalI/HindIII (New England Biolabs, NEB) and cloned into the corresponding sites of pCA24N, generating pCA24N-yafW-ykfI, pCA24N-yfjZ-ypjF, pCA24N-CP4-6-L5, and pCA24N-CP4-57-R5. The constructs were confirmed by PCR followed by DNA sequencing using primers pCA24N-F/-R. The other recombinant plasmids of pBAD, pHGE, pUT18C, and pHERD20T were constructed following similar steps. The detailed information of primer pairs used for PCR amplification, restriction enzyme sites used in digestion of the PCR products, and the primers used for PCR sequencing are shown in Supplementary Table S3. The recombinant plasmids pKT25-ftsZ, pKT25-mreB, pET28b-yafW, pET28b-yfjZ, and pET28b-cbeA were constructed with ClonExpress^TM II One Step Cloning Kit (Vazyme Biotech, Piscataway, NJ, USA), and the correct constructs were confirmed by PCR using primer pairs pKT25-F/-R or T7-F/-R.