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Anti human cd45 antibody

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The Anti-human CD45 antibody is a laboratory reagent used to detect and quantify CD45-positive cells in various research applications. CD45 is a transmembrane protein tyrosine phosphatase expressed on the surface of most hematopoietic cells, making it a useful marker for the identification and analysis of different cell types. This antibody can be used in flow cytometry, immunohistochemistry, and other immunoassays to study the presence and distribution of CD45-expressing cells.

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Lab products found in correlation

Anti mouse cd45 antibody, by BD (2 mentions) Anti human cd45 antibody, by Agilent Technologies (1 mentions) Phosal 50 pg, by Lipoid (1 mentions) Polyethylene glycol 400, by Spectrum Chemical (1 mentions) F4 80, by Thermo Fisher Scientific (1 mentions) Anti mouse cd45, by Thermo Fisher Scientific (1 mentions) Ack lysing buffer, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-human CD45-APC antibody Mouse anti-human CD45 antibody PE-CY7-conjugated anti-human CD45 antibody FITC anti-human CD45 antibody Anti-human CD45-PE antibody Murine anti-human CD45 BV421 clone HI30 monoclonal antibody Anti-human CD45 Anti-CD45 antibody Anti-CD45

7 protocols using anti human cd45 antibody

1

Efficacy of VS-4718 in Xenograft AML Model

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Animal experiments were performed in accordance with a protocol approved by the Institutional Animal Care and Use Committee at MDACC. Molm14 cells (0.6 × 106) stably expressing a dual luciferase-GFP reporter (Molm14-GFP/Luc) were injected via the tail vein into NOD/SCID IL2Rγ Null-3/GM/SF (NSGS) mice (Jackson Laboratory, Bar Harbor, ME). Once engraftment was confirmed by the IVIS-200 noninvasive bioluminescence in vivo imaging system (Xenogen, Hopkinton, MA), mice were either untreated or treated with VS-4718 twice a day at 75 mg/kg via oral gavage (n = 10/group) for 16 days. Leukemia burden was monitored by IVIS in vivo imaging, flow cytometric measurement of human CD45 cells (anti-human CD45 antibody, BD Biosciences) in mouse PB, and immunohistochemical staining for human CD45+ cells in mouse tissues (stained with anti-human CD45 antibody and visualized by Biotin-free Tyramide Signal Amplification System, both from Dako, Carpinteria, CA). Mouse survival was recorded.
Carter B.Z., Mak P.Y., Wang X., Yang H., Garcia-Manero G., Mak D., Mu H., Ruvolo V., Qiu Y., Coombes K., Zhang N., Ragon B., Weaver D.T., Pachter J.A., Kornblau S, & Andreeff M. (2017). Focal Adhesion Kinase as a Potential Target in AML and MDS. Molecular cancer therapeutics, 16(6), 1133-1144.
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2

Leukemia xenograft mouse model

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Xenograft mice models of human cancer cell lines were developed using NOG mice. For leukemia mice models, 2.5 × 106 cells/body of MV4-11 cells were intravenously injected. Peripheral blood (PB) was then collected every week and chimerism was checked by flow cytometer using anti-human CD45 antibody (BD Biosciences). One week after injection, mice were treated with PI polyamides (320 μg/kg body weight, twice a week IV injections) or with the equivalent amount of dimethyl sulfoxide (DMSO).
Morita K., Noura M., Tokushige C., Maeda S., Kiyose H., Kashiwazaki G., Taniguchi J., Bando T., Yoshida K., Ozaki T., Matsuo H., Ogawa S., Liu P.P., Nakahata T., Sugiyama H., Adachi S, & Kamikubo Y. (2017). Autonomous feedback loop of RUNX1-p53-CBFB in acute myeloid leukemia cells. Scientific Reports, 7, 16604.
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3

Evaluating Leukemia Treatments in PDX Models

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Animal experiments were performed in accordance with a protocol approved
by the Institutional Animal Care and Use Committee at MD Anderson. PDX cells (2
× 106) were injected via tail vein into 40 male (16–20
or 5–9 wk) NSGS mice (Jackson Laboratory, Bar Harbor, ME). Engraftment
was confirmed by flow cytometry measuring human CD45+ cells
(anti-human CD45 antibody, BD Biosciences) (average 1%) in mouse peripheral
blood (PB). The mice were treated with vehicle only, VS-4718 twice daily (75
mg/kg) (8 ), ABT-199 daily (100 or 50
mg/kg), or the combination via oral gavage (n = 10/group) for 29 or 27 d.
VS-4718 was suspended in 5% sucrose. ABT-199 was dissolved in 60% PHOSAL 50PG
(LIPOID, Germany), 30% polyethylene glycol 400 (Spectrum Chemical, Gardena, CA),
and 10% 200-proof ethyl alcohol (Pharmco-Aaper, Brookfield, CT).
The leukemia progression was monitored weekly by flow cytometric
measurement of human CD45+ cells in mouse PB. At the end of the
treatments, BM cells were collected from one of the PDX models (PDX1, Supplemental Table 1,
#14) (n = 3 per treatment group) for RNA-sequencing analysis and spleen were
harvested from both PDX models (n = 3/group). Mice were monitored daily and
survival was recorded.
Wang X., Mak P.Y., Mu H., Tao W., Rao A., Visweswaran R., Ruvolo V., Pachter J.A., Weaver D.T., Andreeff M., Xu B, & Carter B.Z. (2020). Combinatorial inhibition of focal adhesion kinase and BCL-2 enhances anti-leukemia activity of venetoclax in acute myeloid leukemia. Molecular cancer therapeutics, 19(8), 1636-1648.
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4

Multiparameter Flow Cytometry Analysis

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Anti-mouse CD45, Ly6G, Ly6C and F4/80 antibodies were purchased from ThermoFisher (Waltham, MA, USA). Anti-CD11b antibody was from Tonbo Biosciences (San Diego, CA, USA). Anti-human CD45 antibody was from BD Bioscience (San Jose, CA, USA). Cy5 fluorescent dye-conjugated T1 and scramble aptamers were ordered from Integrated DNA Technologies (Coralville, IA, USA).
Li J., Mai J., Hinkle L., Lin D., Zhang J., Liu X., Ramirez M.R., Zu Y., Lokesh G.L., Volk D.E, & Shen H. (2019). Tracking Biodistribution of Myeloid-Derived Cells in Murine Models of Breast Cancer. Genes, 10(4), 297.
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5

Antibody-based Cell Identification

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Anti-human CD45 antibody and anti-mouse CD45 antibody were from BD Biosciences (San Jose, CA).
Sugimoto K., Hayakawa F., Shimada S., Morishita T., Shimada K., Katakai T., Tomita A., Kiyoi H, & Naoe T. (2015). Discovery of a drug targeting microenvironmental support for lymphoma cells by screening using patient-derived xenograft cells. Scientific Reports, 5, 13054.
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6

Analyzing Leukemia Burden in CCRF Xenografts

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To assess the level of leukemia burden in the mice transplanted with CCRF, cells from BM, spleen, liver, and PB were lysed with ACK Lysing Buffer (Gibco) and then buffered with PBS and 1% FBS before analysis. Flow cytometry quantification of hCD45+ cells in CCRF xenograft models was performed using an anti–human CD45 antibody (BD Biosciences). All data were analyzed with FlowJo program (version 10).
Wang R., Zhang H., Xu J., Zhang N., Pan T., Zhong X., Zhang H., Yin L., Yao Y., Wu Q., Li Z., Liu X., Xu K, & Niu M. (2020). MALT1 Inhibition as a Therapeutic Strategy in T-Cell Acute Lymphoblastic Leukemia by Blocking Notch1-Induced NF-κB Activation. Frontiers in Oncology, 10, 558339.
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7

Porcine and Human Immune Cell Engraftment Tracking

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Adoptively transferred PBMC were followed in recipient mice in blood collected on days 14 and 28. fluorescence-activated cell sorter (FACS) analysis was performed using anti-porcine (Serotec, Raleigh, NC) (Figure 4A andB) or anti-human CD45 antibody (BD, Heidelberg, Germany) (Figure 7A andB), and anti-mouse CD45 antibody (BD, Franklin Lakes, NJ). Successful engraftment, as required for inclusion into final analysis, was assumed when more than 2% of the circulating lymphocytes were porcine (Figure 4B) or human CD45 + (Figure 7B), respectively. 8
Sommer W., Knöfel A.K., Madrahimov N., Avsar M., Jonigk D., Salman J., Dreckmann K., Jansson K., Salguero G., Maus U.A., Welte T., Haverich A, & Warnecke G. (2015). Allogeneic CD4+CD25high T cells regulate obliterative bronchiolitis of heterotopic bronchus allografts in both porcinized and humanized mouse models. Transplantation, 99(3).
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