EGFR TKIs Gefitinib, Erlotinib, Dacomitinib, and Afatinib were purchased from Selleckchem (Houston, TX). Cell proliferation reagent WST-1, Dimethyl Sulfoxide (DMSO), Chloroquine, Spautin-1, and Flavopiridol were from Sigma-Aldrich (St. Louis, MO). Antibodies to LC3, phosphor-ULK1 (Ser317), phosphor-ULK1 (Ser757), total ULK1, phosphor-p70 S6K, Cathepsin D were from Cell Signaling Technology (Danvers, MA). Antibodies to Beclin-1, GAPDH, Tubulin, and total p70 S6K were from Santa Cruz Biotechnology (Santa Cruz, CA). Antibody to actin was from MP Biomedicals (Santa Ana, CA). Secondary antibodies were from Jackson ImmunoResearch Laboratories (West Grove, PA). DQ-Red BSA was obtained from ThermoFisher Scientific (Waltham, MA).
Total p70 s6k
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Total p70 S6K is a lab equipment product that measures the total levels of the p70 S6 kinase (p70 S6K) protein in biological samples. p70 S6K is a key regulator of protein synthesis and cell growth. This product can be used to quantify the total p70 S6K protein in a sample, which provides information about the activation status of the mTOR signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Dacomitinib, by Selleck Chemicals (1 mentions)
Flavopiridol, by Merck Group (1 mentions)
Afatinib, by Selleck Chemicals (1 mentions)
Erlotinib, by Selleck Chemicals (1 mentions)
Chloroquine, by Merck Group (1 mentions)
Spautin 1, by Merck Group (1 mentions)
Cathepsin d, by Cell Signaling Technology (1 mentions)
Gefitinib, by Selleck Chemicals (1 mentions)
Dq red bsa, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
P 4e bp1, by Santa Cruz Biotechnology (1 mentions)
Total eif4e, by Santa Cruz Biotechnology (1 mentions)
Immobilon western chemiluminescent hrp substrate, by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using total p70 s6k
1
EGFR TKIs Modulate Autophagy
2
Immunoblotting Analysis of Protein Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Identical amounts of protein from cultured HepG2 cells and frozen liver tissue homogenates were separated by SDS-PAGE, transferred to PVDF membranes, and immunoblotted with primary antibodies followed by incubation with an HRP-conjugated secondary antibody. Finally detection procedures were performed using Immobilon Western Chemiluminescent HRP Substrate (Millipore, Temecula, CA, USA). Primary antibodies against the following proteins were used: CD36, total p70S6K, p-p70S6K (Thr 421/Ser 424), total 4E-BP1, p-4E-BP1 (Ser 65/Thr 70), total eIF4E, p-eIF4E (Ser 209) and β-actin (Santa Cruz, Dallas, TX, USA); total mTOR (Millipore, Temecula, CA, USA); and p-mTOR (phospho S2448) (Abcam, Cambridge, UK).
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