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Yeast extract

Manufactured by Molar Chemicals
Sourced in Hungary

Yeast extract is a laboratory ingredient used as a nutrient source for microbial cultures. It is derived from the autolysis or enzymatic hydrolysis of yeast cells. Yeast extract contains a variety of vitamins, amino acids, and other growth factors that support the growth and proliferation of microorganisms in cell culture and fermentation applications.

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2 protocols using yeast extract

1

Microbiological media components protocol

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The following microbiological media components were used: Bacto malt extract was purchased from Becton Dickinson (Környe, Hungary) and yeast extract, NaCl and sucrose were purchased from Molar Chemicals (Halásztelek, Hungary). All solvents used for extraction were purchased from Molar Chemicals Hungary and were of analytical grade. The solvents used for the various solvent systems and HPLC-UV measurements were of gradient grade or super gradient grade and were purchased from VWR International (Debrecen, Hungary). The AF standard mixture (including AFB1, AFG1, AFB2 and AFG2) was purchased from Sigma-Aldrich (Budapest, Hungary).
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2

Plasmid Construction and Purification

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Restriction enzymes, T4 ligase, TranscriptAid T7 High Yield Transcription Kit, Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum, Turbofect and Penicillin/Streptomycin were purchased from Thermo Fischer Scientific. DNA oligonucleotides and the GenElute HP Plasmid Miniprep kit used in plasmid purifications were acquired from Sigma-Aldrich. T4 Polynucleotide Kinase, Q5 High-Fidelity DNA Polymerase, NEB Stable Competent E. coli, HiFi Assembly Master Mix were from New England Biolabs Inc. NucleoSpin Gel and PCR Clean-up kit used to clean up DNA from agarose gels were purchased from Macherey-Nagel. ZymoPURE Plasmid Midiprep Kit and RNA Clean & Concentrator Kit were from Zymo Research. Ampicillin, kanamycin and chloramphenicol antibiotics were from Sigma-Aldrich, NaCl, yeast extract and tetracycline were from Molar chemicals, agarose and tryptone were from VWR.
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