Ml130

Manufactured by Selleck Chemicals

Sourced in United States, Germany

The ML130 is a compact and efficient lab equipment designed for various scientific applications. It features a durable construction and precise temperature control capabilities. The core function of the ML130 is to provide a controlled environment for temperature-sensitive experiments and processes.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using ml130

Airway Epithelial Cell Immune Response

Check if the same lab product or an alternative is used in the 5 most similar protocols

Airway epithelial cells were cultured under ALI conditions as described and washed with BEBM before treatment. Cells were treated with iE-DAP (50ug/ml, Invivogen, San Diego CA), iE-LYS (50ug/ml; Invivogen, San Diego CA) or negative control media for 24 hr prior to supernatant harvest. For NOD1 inhibition, cells were pretreated with ML130 (1uM, Selleckchem, Houston, TX) for 1hr at 37°C, 5% CO₂ before addition of iE-DAP or H. pylori. Similar procedures were performed using NF-kappaB inhibitor JSH-23 (10uM, ApexBio Tech, TX) and p38 MAP kinase inhibitor SB203580 (10uM, Selleckchem, Houston, TX) prior to addition of H. pylori.

dela Pena-Ponce M.G., Jimenez M.T., Hansen L.M., Solnick J.V, & Miller L.A. (2017). The Helicobacter pylori type IV secretion system promotes IL-8 synthesis in a model of pediatric airway epithelium via p38 MAP kinase. PLoS ONE, 12(8), e0183324.

+ Open protocol

+ Expand

Ginsenoside and Bacterial Sensing Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rg3 was purchased from (Sigma, St. Louis, USA) and dissolved in dimethyl sulfoxide. Ginsenoside Rb1 was obtained from the Korea Ginseng and Tobacco Research Institute (Daejeon, Korea) and dissolved in 100% ethanol. ML130 was purchased from Selleckchem (Houston, USA) and iE-DAP was purchased from InvivoGen (San Diego, USA). Rg3, Rb1, iE-DAP, and ML130 were used at the indicated doses and time points.

Lee A., Yun E., Chang W, & Kim J. (2019). Ginsenoside Rg3 protects against iE-DAP–induced endothelial-to-mesenchymal transition by regulating the miR-139-5p–NF-κB axis. Journal of Ginseng Research, 44(2), 300-307.

+ Open protocol

+ Expand

Lysosomal Lipid Storage Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Small-molecule inhibitors were added to cell culture medium 1 h prior to stimulation. ML-130 (5 µM) and ponatinib (50 nM) were purchased from Selleck Chemical. Compound 21a (XIAP-Cp-21a, 1–2.5 µM) was kindly provided by TetraLogics Pharmaceuticals.26–28 (link) To induce the lysosomal lipid storage phenotype in MDM/PBMC, cells were pre-incubated with 2 µg/mL U18666A (Sigma-Aldrich) for 48/24 h. Other compounds were bafilomycin A1 (50 nM, Enzo Life Sciences), 2-hydroxypropyl-β-cyclodextrin (0.5–2%, Sigma-Aldrich), chlorpromazine (1–10 μg/mL, Merck Millipore), rapamycin (1–10 µM, Cayman Chemical), Torin 1 (10 µM, Tocris), d-(+)-trehalose dihydrate (100 mM, Sigma-Aldrich) and miglustat (4–400 µM, Tocris).

Schwerd T., Pandey S., Yang H.T., Bagola K., Jameson E., Jung J., Lachmann R.H., Shah N., Patel S.Y., Booth C., Runz H., Düker G., Bettels R., Rohrbach M., Kugathasan S., Chapel H., Keshav S., Elkadri A., Platt N., Muise A.M., Koletzko S., Xavier R.J., Marquardt T., Powrie F., Wraith J.E., Gyrd-Hansen M., Platt F.M, & Uhlig H.H. (2016). Impaired antibacterial autophagy links granulomatous intestinal inflammation in Niemann–Pick disease type C1 and XIAP deficiency with NOD2 variants in Crohn's disease. Gut, 66(6), 1060-1073.

+ Open protocol

+ Expand

Comprehensive Immune Receptor Agonist Library

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following compounds were purchased from Invivogen (Ibian Technologies, Zaragoza, Spain): NOD1 and NOD2 agonist kit (#tlrl-nodkit2), containing C12-iE-DAP, iE-DAP, murmyl-dypeptide (MDP), L18-MDP, M-TriDAP, M-TriLYS, murabutide, PGN-ECndi and TriDAP, were purchased from Invivogen (Ibian Technologies, Zaragoza, Spain); Human TLR3/7/8/9 Agonist Kit (#TLRL-KIT3HW3), containing Poly(I:C) (HMW), Poly(I:C) (LMW), Poly(A:U), Imiquimod, R848, CL075, ssRNA40/LyoVec, ssRNA41/LyoVec, ODN2006, ODN2006control, ODN2216, ODN2216control, ODN2395 and ODN2395control; CU-CPT9a, ODN2088, MRT67307, BX795, BAY 11-7082, Pepinh-MYD, Pepinh-MYD Control, Pepinh-TRIF, Pepinh-TRIF Control, L-Kynurenine, 3p-hpRNA, G3-YSD, G3-YSD Control, H-151, G-140, Indirubin, VACV-70/LyoVec, VACV-70c control, HSV-60, Control for CDS Ligands HSV-60/LyoVec, Poly(dA:dT) LyoVec and Poly(dG:dC) LyoVec. NOD inhibitors NOD-IN-1 and ML130 were purchased from Selleckchem (Munich, Germany). Poly(I:C), lipopolysaccharide (LPS, #L6529) and PMA (#P1585) were obtained from Sigma-Merck, Burlington, MA, USA. All compounds were reconstituted in dimethyl sulfoxide (DMSO) or water according to manufacturer’s instructions and stored at −20 °C until use.

Garcia-Vidal E., Calba I., Riveira-Muñoz E., García E., Clotet B., Serra-Mitjà P., Cabrera C., Ballana E, & Badia R. (2024). Nucleotide-Binding Oligomerization Domain 1 (NOD1) Agonists Prevent SARS-CoV-2 Infection in Human Lung Epithelial Cells through Harnessing the Innate Immune Response. International Journal of Molecular Sciences, 25(10), 5318.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!