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Cflow plus program software

Manufactured by BD
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CFlow Plus is a program software developed by BD for lab equipment. It serves as the core function to manage and operate the associated lab instruments.

Automatically generated - may contain errors

Lab products found in correlation

Accuri c6, by BD (4 mentions) Fcs express, by De Novo Software (2 mentions) Annexin 5 alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Accuri c6 flow cytometer, by BD (2 mentions) Sytox red, by Thermo Fisher Scientific (1 mentions) Pi rnase, by Merck Group (1 mentions)

Close spelling variants of this product

CFlow Plus program CFlow Plus software CFlow Plus CFlow software CFlow

6 protocols using cflow plus program software

1

Apoptosis Assay for BT474 Cells

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Example 9

After treating BT474 cells with different concentrations of HS-106 for 24 hours, the annexin V assay was executed as previously described (Safi et al., 2014). Briefly, cells were collected and stained with Alexa Fluor 488 Annexin V and Sytox Red according to the manufacturer's protocol. Annexin V-positive cells were considered apoptotic, and their percentage of the total number of cells was calculated. Ten thousand events were collected for each sample using a BD Accuri C6 flow cytometer (BD), and data were analyzed using the CFlow Plus program software (BD) and FCS express (De Novo Software).

US10966981B2. Fatty acid synthase inhibitors (2021-04-06). Duke University [US], Ohio State Innovation Foundation [US]. Inventors: Jesse Kwiek [US], Timothy Haystead [US], Philip Hughes [US], Yazan Alwarawrah [US].
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2

Apoptosis Assay in Prostate Cancer Cells

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LNCaP and 22RV1 cells (3 × 105 cells/well) were seeded in six-well plates and treated accordingly. Cells were then harvested and double stained with Alexa Flour 488 Annexin V and Sytox according to the manufacturers’ instructions. Annexin V positive cells were considered apoptotic and the percentage of total cell number was calculated. A minimum of 10,000 events were collected per sample using a BD Accuri C6 flow cytometer and data were analyzed using the CFlow plus program software (BD Biosciences, San Jose, CA).
Alfaqih M.A., Nelson E.R., Liu W., Safi R., Jasper J.S., Macias E., Geradts J., Thompson J.W., Dubois L.G., Freeman M.R., Chang C.Y., Chi J.T., McDonnell D.P, & Freedland S.J. (2017). CYP27A1 loss dysregulates cholesterol homeostasis in prostate cancer. Cancer research, 77(7), 1662-1673.
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3

Apoptosis Quantification in VCaP Cells

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VCaP cells were collected and double stained with Alexa Fluor 488 Annexin V and Sytox (Invitrogen) according to the manufacturer’s instruction. Annexin V-positive cells were considered apoptotic, and their percentage of the total number of cells was calculated. Ten thousand events were collected for each sample using a BD Accuri C6 flow cytometer (BD San Jose, CA) and data were analyzed using the CFlow Plus program software (BD San Jose, CA).
Safi R., Nelson E.R., Chitneni S.K., Franz K.J., George D.J., Zalutsky M.R, & McDonnell D.P. (2014). Copper Signaling Axis as a Target for Prostate Cancer Therapeutics. Cancer research, 74(20), 5819-5831.
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4

Apoptosis Assay in BT474 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 9

After treating BT474 cells with different concentrations of HS-106 for 24 hours, the annexin V assay was executed as previously described (Safi et al., 2014). Briefly, cells were collected and stained with Alexa Fluor 488 Annexin V and Sytox Red according to the manufacturer's protocol. Annexin V-positive cells were considered apoptotic, and their percentage of the total number of cells was calculated. Ten thousand events were collected for each sample using a BD Accuri C6 flow cytometer (BD), and data were analyzed using the CFlow Plus program software (BD) and FCS express (De Novo Software).

US11679111B2. Fatty acid synthase inhibitors (2023-06-20). Duke University [US], Ohio State Innovation Foundation [US]. Inventors: Jesse Kwiek [US], Timothy Haystead [US], Philip Hughes [US], Yazan Alwarawrah [US].
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5

Apoptosis Quantification in Prostate Cancer Cells

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VCaP (3 × 105 cells/well) and 22Rv1 (1.2 × 105 cells/well) were plated in DMEM containing 8% FBS and the indicated compound for 24 to 72 hours. Cells were trypsinized, washed, and double stained with Alexa Fluor 488 Annexin V and Sytox Red (Invitrogen) according to the manufacturer's instructions. Percentage of apoptotic (Annexin V positive) cells within the total number of cells was calculated. A total of 10,000 events were collected for each sample (BD Accuri C6 flow cytometer) and data were analyzed using the CFlow Plus program software (BD Biosciences).
Stice J.P., Wardell S.E., Norris J.D., Yllanes A.P., Alley H.M., Haney V.O., White H.S., Safi R., Winter P.S., Cocce K.J., Kishton R.J., Lawrence S.A., Strum J.C, & McDonnell D.P. (2017). CDK4/6 Therapeutic Intervention and Viable Alternative to Taxanes in CRPC. Molecular cancer research : MCR, 15(6), 660-669.
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6

Cell Cycle Analysis by Flow Cytometry

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Cells were seeded in media containing 8% FBS between 1.2 × 105 and 3 × 105 cells per well in 12-well plates and allowed to grow for 48 hours before addition of the indicated treatments in duplicate. Cells were harvested and fixed in 70% (v/v) cold ethanol at −20° C overnight. After washing (PBS), fixed cells were collected (centrifugation) and resuspended in PI/RNase (Sigma) for DNA staining and analysis (BD Accuri C6 flow cytometer). Data were analyzed using the CFlow Plus program software (BD Biosciences).
Stice J.P., Wardell S.E., Norris J.D., Yllanes A.P., Alley H.M., Haney V.O., White H.S., Safi R., Winter P.S., Cocce K.J., Kishton R.J., Lawrence S.A., Strum J.C, & McDonnell D.P. (2017). CDK4/6 Therapeutic Intervention and Viable Alternative to Taxanes in CRPC. Molecular cancer research : MCR, 15(6), 660-669.
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