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Human recombinant il 23

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Human recombinant IL-23 is a cytokine that plays a key role in the regulation of immune responses. It is a heterodimeric protein composed of two subunits, p19 and p40. IL-23 is involved in the differentiation and expansion of Th17 cells, which are important for the immune response against extracellular pathogens.

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Lab products found in correlation

Dynabeads human t cell activator cd3cd28, by Thermo Fisher Scientific (2 mentions) Soluble anti cd28 mab, by BioLegend (2 mentions) Human recombinant tgfβ1, by BioLegend (2 mentions)

Spelling variants of this product

IL-23 (65 citations) Recombinant IL-23 (4 citations)

2 protocols using human recombinant il 23

1

In vitro generation of human Tfh cells

Cited 1 time
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Human Tfh cells were generated as previously described42 (link). Briefly, after overnight stimulation of naive CD4+ T cells with Dynabeads human T cell activator CD3/CD28 (Thermo Fisher) in complete RPMI medium, cells were transferred to 96-well plates coated with anti-CD3 and supplemented with 2 μg/ml of soluble anti-CD28 mAb (Biolegend), human recombinant IL-23 (25 ng/ml; Biolegend) and human recombinant TGFβ1 (5 ng/ml; Biolegend). After 4 d cells were collected and used for analysis.
Caielli S., Troggian Veiga D., Balasubramanian P., Athale S., Domic B., Murat E., Banchereau R., Xu Z., Chandra M., Chung C.H., Walters L., Baisch J., Wright T., Punaro M., Nassi L., Stewart K., Fuller J., Ucar D., Ueno H., Zhou J., Banchereau J, & Pascual V. (2018). A CD4+ T cell population expanded in Lupus blood provides B cell help through IL10 and succinate. Nature medicine, 25(1), 75-81.
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2

In vitro generation of human Tfh cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Human Tfh cells were generated as previously described42 (link). Briefly, after overnight stimulation of naive CD4+ T cells with Dynabeads human T cell activator CD3/CD28 (Thermo Fisher) in complete RPMI medium, cells were transferred to 96-well plates coated with anti-CD3 and supplemented with 2 μg/ml of soluble anti-CD28 mAb (Biolegend), human recombinant IL-23 (25 ng/ml; Biolegend) and human recombinant TGFβ1 (5 ng/ml; Biolegend). After 4 d cells were collected and used for analysis.
Caielli S., Troggian Veiga D., Balasubramanian P., Athale S., Domic B., Murat E., Banchereau R., Xu Z., Chandra M., Chung C.H., Walters L., Baisch J., Wright T., Punaro M., Nassi L., Stewart K., Fuller J., Ucar D., Ueno H., Zhou J., Banchereau J, & Pascual V. (2018). A CD4+ T cell population expanded in Lupus blood provides B cell help through IL10 and succinate. Nature medicine, 25(1), 75-81.
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