β glucanase

The chemicals maltodextrin, colloidal silicon dioxide, (S)-(-)-6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox®), Folin-Ciocalteu, gallic acid, aluminum trichloride (AlCl₃), 2, 4, 6 - tripyridyl −1, 3, 5 - triazine (TPTZ), calcium chloride (CaCl₂), α-amylase, pepsin, hydrochloric acid (HCl), pancreatin, bile, sodium hydroxide (NaOH), protease, Viscozyme (arabinase, cellulase, β-glucanase, hemicellulase and xylanase) were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA) and HPLC reagents were purchased from Merck KGaA (Darmstadt, Germany).

Barley seed (cultivar Yangmai 3) was obtained from the plant genetic engineering laboratory of Shanghai Academy of Agricultural Sciences. The transgenic seedlings were transplanted to a paddy field in Baihe county, Qingpu district, Shanghai, China. Standard enzymes (phytase, β‐glucanase, and xylanase) and sodium phytate were purchased from Sigma Chemical Co., Ltd (St. Louis, MO, USA). All other chemicals were analytical grade and were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All primers were purchased from Shanghai Sangon Biological Engineering Technology and Service Co., Ltd. (Shanghai, China). Agrobacterium tumefaciens strain EHA105 and Escherichia coli DH5α were obtained from the plant genetic engineering laboratory of Shanghai Academy of Agricultural Sciences. An RNA isolation kit was purchased from Fermentas International, Inc. (Burlington, ON, Canada), and a reverse Transcription System was purchased from Promega (Madison, WI). Twenty‐four 4‐week‐old black‐feathered laying hens (Xingyang) were obtained from the National Poultry Engineering Research Center of the Ministry of Agriculture (Shanghai, China).