Microsomes for the immunoassay determination were isolated and proteins extracted as described by Calvo-Polanco et al. (2014) (link). As primary antibodies we used two antibodies (at a dilution of 1:1000) that recognize several PIP1s and PIP2s and three antibodies that recognize the phosphorylation of PIP2 proteins at their C-terminal region at serine 280 (PIP2280), serine 283 (PIP2283), and at both serine 280 and 283 (PIP2280/283). Goat anti-rat IgG coupled with horseradish peroxidase (Sigma-Aldrich) was used as the secondary antibody for PIP1 (at 1:10 000). Goat anti-rabbit Ig coupled to horseradish peroxidase (Sigma-Aldrich) was used as a secondary antibody for PIP2 and PIP2280, PIP2283, and PIP2280/283 (at 1:10000). Protein quantification was done in three different independent root samples per treatment, repeated three times each.
Goat anti rabbit ig
Manufactured by Merck Group
Goat anti-rabbit Ig is a secondary antibody used in various immunoassay and immunodetection techniques. It is produced by immunizing goats with rabbit immunoglobulins (Ig) and purifying the resulting antibodies. This product can be used to detect and quantify rabbit primary antibodies in sample preparations.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti mouse igg cy 5, by Jackson ImmunoResearch (1 mentions)
Goat anti rabbit igg cy 5, by Jackson ImmunoResearch (1 mentions)
Cy3 goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Goat anti mouse igg cy3, by Jackson ImmunoResearch (1 mentions)
Rat anti ha, by Roche (1 mentions)
Mouse anti flag, by Merck Group (1 mentions)
Mouse anti myc, by Roche (1 mentions)
2 protocols using goat anti rabbit ig
1
Immunoassay Determination of PIP1 and PIP2
2
Antibody Detection and Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The antbodies used are as follows: rabbit anti-STREP horseradish peroxidase (1:10,000, BioRad, 161-0380), Mouse anti-Gephyrin 3B11 (1:1,000, SySy, 147111), mouse anti-gephyrin mAb7a (1:2,000, SySy, 147021), rabbit anti-vGAT (1:3,000, SySy, 131003), mouse anti-vGAT (1:5,000, SySy, 131011), rat anti-HA (1:3,000, Roche), mouse anti-Myc (1:5,000, Roche, 11667149001), mouse anti-FLAG (1:5,000, Sigma-Aldrich, F3165), rabbit anti-GFP (1:5,000, Synaptic Systems, 132002), chicken anti-GFP (1:5,000, Chemicon, AB16901), mouse anti SUMO-1 (1:1,000, 21C7, Hybridoma bank), mouse anti SUMO2/3 (1:1,000, 8A2, Hybridoma Bank), rabbit anti-SUMO1 (1:250, Epitomics, 1563-1), rabbit anti-SUMO2/3 (1,250, Epitomics, 2970-1), rabbit anti pan-Acetylated Lysine antibody (1:1,000, Cell Signaling, 9441) and mouse anti-Ac-Lysine (AKL5C1) (1:2,000, Santa Cruz, Sc32268). Gephyrin anti-S270 phospho-antibody has been described earlier28 (link). The following secondary antibodies were used: goat anti-mouse IgG Cy3 (1:500, Jackson Immunoresearch, 115165), goat anti-mouse IgG Cy5 (1:500, Jackson Immunoresearch, 115175), goat anti-rabbit IgG Cy3 (1:500, Jackson Immunoresearch, 111165), goat anti-rabbit IgG Cy5 (1:500, Jackson Immunoresearch, 111175), goat anti-mouse IgG alkaline phosphatase (AP) (1:30,000, Sigma-Aldrich, A3562) and goat anti-rabbit IgG AP (1:30,000, Sigma-Aldrich, A3687).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!