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Accu chek compact plus blood glucose meter

Manufactured by Roche
Sourced in Japan

The Accu-Chek Compact Plus Blood Glucose Meter is a handheld device that measures the amount of glucose in a small drop of blood. It provides accurate and reliable results to assist in the management of diabetes.

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5 protocols using accu chek compact plus blood glucose meter

1

Glucose Tolerance Test in Mice

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Mice were fasted overnight (~16 hours), followed by an IP injection of 2 mg D-glucose per gram mouse weight. Tail vein blood samples were collected at 0, 15, 30, 60, 90, and 120 minutes after the injection and glucose concentration was determined using the Accu-Chek Compact Plus Blood Glucose Meter (Roche).
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2

Insulin Tolerance Test in Mice

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Mice were fasted for 5 hours starting at 9 am. Insulin (Humalog in PBS) was injected IP at 0.75 units per kg mouse weight. Tail vein blood samples were collected at 0, 15, 30, 60, 90, and 120 minutes after the injection and glucose concentration was determined using the Accu-Chek Compact Plus Blood Glucose Meter (Roche). To measure plasma glucagon during an insulin tolerance test, blood was collected into heparinized tubes immediately before, and 30 minutes after, an insulin injection. Plasma was separated from whole blood by centrifugation and glucagon concentration was measured by ELISA (Mercodia).
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3

Glucose and Insulin Tolerance Tests

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To perform the gluclose tolerance test (GTT) or insulin tolerance test (ITT), mice were fasted overnight before receiving an i.p. injection of 20% glucose (2 g/kg body weight) or recombinant insulin (Humulin R, 0.75 U/kg; Lily), respectively. Blood samples were collected from the tail 0, 0.5, 1, 2, and 4 hours later, after injection for analysis using the Accu-Chek Compact Plus blood glucose meter (Roche Diagnostics).
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4

Streptozotocin-Induced Diabetes in Rats

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For the purpose of acclimatization and reaching optimal weight (at least 250 gr), all of the rats were kept in an animal lab for two weeks prior to the experiments. Subsequently, following an overnight fast, diabetes was induced through a single intraperitoneal injection of streptozotocin (STZ) (45 mg/kg; Sigma, St. Louis, MO) solution (dissolved in 0.5 mol/L citrate buffer at pH 4.0). Two days later, diabetes was confirmed through measuring tail vein blood glucose level (>300 mg/dL) by Accu-Chek Compact Plus blood glucose meter (Roche Diagnostics K.K., Tokyo, Japan). In this regard, blood glucose levels were controlled throughout the study, once every week (Fig. 1).

Study design.

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5

Fasting Blood Glucose and Insulin Analysis

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Cohorts of mice were fasted overnight for ~16 hours. After the fast, tail vein blood was collected and blood glucose concentration was determined using the Accu-Chek Compact Plus Blood Glucose Meter (Roche). To determine insulin plasma concentration, fasted blood was collected into heparinized tubes. Plasma was separated from whole blood by centrifugation and insulin concentration was measured by ELISA (Mercodia). HOMA-IR was determined by the formula: HOMA-IR = fasting plasma insulin (μU/L) * fasting blood glucose (mmol/L) / 22.5.
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