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Mab2018

Manufactured by Merck Group

MAB2018 is a lab equipment product manufactured by Merck Group. It serves as a general-purpose tool for laboratory applications. The core function of MAB2018 is to perform specific tasks required in a laboratory setting.

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3 protocols using mab2018

1

Immunohistochemical Profiling of Brain Regions

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Brains were processed for immunohistochemistry in the same fashion as for in situ hybridization on sections. Brain sections were stained with DAPI and incubated with the following primary antibodies: rabbit anti-Foxp2 (1:200, Abcam, ab16046), mouse anti-Nkx2.2 (1:300, DSHB, 74.5A), rabbit anti-Olig2 (1:300, Millipore, AB9610), mouse anti-Sox2 (1:100, R&D Systems, MAB2018), rabbit anti-Sox2 (1:300, Millipore, Cat#AB5603), and rabbit anti-Tyrosine Hydroxylase (1:1000, Pel-Freez, P40101–0). Detection of primary antibodies was achieved using secondary antibodies conjugated to goat anti-mouse Alexa 488 (1:400, Thermo Fisher, Cat#A28175), goat anti-rabbit Alexa 488(1:400, Thermo Fisher, Cat#A-11008), and goat anti-rabbit Alexa 594 (1:400, Thermo Fisher, Cat#A-11037). Specimens were imaged on a Leica TCS SP8 MP system.
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2

Antibodies and Reagents for Stem Cell Studies

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The following antibodies and drugs were used in this study: anti-OCT4 (Santa Cruz Biotechnology, sc-5279), anti-SSEA4 (Invitrogen, 414000), anti-PAX6 (Covance Research, PRB-278P-100), anti-PAX6 (Abcam, ab78545), anti-Ki67 (Abcam, ab15580), anti-SOX1 (Millipore, AB15766), anti-SOX2 (RD, MAB2018), anti-NESTIN (Millipore, ABD69), anti-NESTIN (BD, 611658), anti-DCX (Cell Signaling, 4604), anti-NKX2.1 (Millipore, MAB5460), anti-FOXG1 (Abcam, ab18259), anti-COUP-TF1 (Millipore, ABE1425), anti-HNA (Millipore, MAB1281), anti-STEM121 (Takara, Y40410), anti-NEUN (Millipore, ABN78), anti-GFAP (Dako, Z0334), anti-GFAP (Millipore, MAB360), anti-SATB2 (Abcam, ab51502), anti-CTIP2 (Abcam, ab18465), anti-TBR1 (Abcam, ab31940), anti-MAP2 (Abcam, ab32454), anti-MAP2 (Millipore, MAB3418), anti-synapsin 1 (Calbiochem, 574777), anti-PSD95 (Abcam, ab13552), Y-27632 (Sigma, S1049), G418 (Sigma, A1720), thiazovivin (Selleck, S1459), SB431542 (Selleck, S1067), dorsomorphin Selleck, S7840), insulin (Gibco. I9278), and heparin (Sigma, H3149).
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3

Immunohistochemical Profiling of Brain Regions

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Brains were processed for immunohistochemistry in the same fashion as for in situ hybridization on sections. Brain sections were stained with DAPI and incubated with the following primary antibodies: rabbit anti-Foxp2 (1:200, Abcam, ab16046), mouse anti-Nkx2.2 (1:300, DSHB, 74.5A), rabbit anti-Olig2 (1:300, Millipore, AB9610), mouse anti-Sox2 (1:100, R&D Systems, MAB2018), rabbit anti-Sox2 (1:300, Millipore, Cat#AB5603), and rabbit anti-Tyrosine Hydroxylase (1:1000, Pel-Freez, P40101–0). Detection of primary antibodies was achieved using secondary antibodies conjugated to goat anti-mouse Alexa 488 (1:400, Thermo Fisher, Cat#A28175), goat anti-rabbit Alexa 488(1:400, Thermo Fisher, Cat#A-11008), and goat anti-rabbit Alexa 594 (1:400, Thermo Fisher, Cat#A-11037). Specimens were imaged on a Leica TCS SP8 MP system.
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