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Goat anti mouse igg fitc conjugate

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Goat anti-mouse IgG-FITC conjugate is a reagent used in immunoassays and flow cytometry applications. It consists of goat-derived antibodies that specifically recognize mouse immunoglobulin G (IgG), conjugated to the fluorescent dye fluorescein isothiocyanate (FITC).

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4 protocols using goat anti mouse igg fitc conjugate

1

Immunofluorescent Localization of T. spiralis Glutamine Synthetase

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Whole worms of different T. spiralis stages (ML, IIL, AW, and NBL) were fixed with 4% formaldehyde for 30 min and re-fixed with cold acetone for 20 min. The fixed worms were embedded in paraffin and cut into a 2-μm-thick cross-section with a microtome. The expression and tissue location of native TsGS in various worm stages were investigated by indirect immunofluorescent assay (IIFA) (Liu et al., 2017 (link); Cui et al., 2019 (link)). Whole parasites and their cross-sections were blocked with 5% normal goat serum for 2 h and then probed at 37°C for 2 h with 1:10 diluted diverse sera (anti-rTsGS serum, infection serum, and normal serum). After washes with PBS, the worms were stained using goat anti-mouse IgG-FITC conjugate (1:100; Santa Cruz, United States). Following washes again, whole worms and cross-sections were examined under a fluorescence microscope (Olympus, Tokyo, Japan) (Lei et al., 2020 (link); Yue et al., 2020 (link)).
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2

Immunofluorescence Staining of TRAF6 and NF-κB

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All the slides containing cells were fixed in 4% paraformaldehyde for 15 min, permeated in 0.1% Triton X-100 for 5 min, and then blocked in 5% goat serum for 1 h. HASMCs were stained or costained with rabbit anti-TRAF6 (Cat#ab62488, Abcam; 1:50) and mouse anti-NF-κB (Cat#sc8008, Santa Cruz; 1:50) overnight at 4°C. The cells were incubated with secondary goat anti-rabbit IgG-PE conjugate (Cat#sc3739, Santa Cruz; 1:100) or goat anti-mouse IgG-FITC conjugate (Cat#sc2010, Santa Cruz; 1:100). The slides were washed 3 times in between each step, and lastly, incubated with anti-fade reagent with DAPI (Cat#8961, CST, USA). The cells were observed under a confocal laser scanning microscopy (LSM710, ZEISS, Germany). We took 4 images for every slide randomly, and repeated all the experiments for at least 3 times.
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3

Immunolocalization of TsSP1.1 in T. spiralis Stages

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Complete worms of diverse T. spiralis developmental stages (ML, 6h and 12h IIL, 3d and 6d AW, and NBL) were fixed with 4% formaldehyde for 30 min. The fixed worms were embedded in paraffin and cut into a 2-μm thick cross-section with a microtome. Expression and tissue location of native TsSP1.1 in diverse stage worms was observed using IIFA. Complete worms and their cross-sections were blocked with 5% normal goat serum for 2 h, and then incubated at 37 °C for 2 h with 1:10 diluted various sera. Following washes with PBS, the worms were stained by goat anti-mouse IgG-FITC conjugate (1:100; Santa Cruz, USA). After washes again, complete worms and cross-sections were observed under fluorescence microscopy (11 (link)).
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4

Localization of Tsgal Protein in T. spiralis

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IFA was used to locate the position of Tsgal protein in T. spiralis. The whole ML, IIL, AW and NBL were fixed with acetone, the ML and AW were also fixed in paraformaldehyde and embedded in paraffin. Then 2-μm sections of this nematode were prepared by using the microtome-cutting. The whole parasites and sections were first blocked with 5% goat serum in PBS and then incubated for 1 h at 37 °C with anti-rTsgal serum diluted at 1:10 [42 (link)]. Pre-immune normal serum was utilized as negative control and infection serum as positive control. Following washes, the whole parasites and sections were incubated with 1:100 dilutions of a goat anti-mouse IgG-FITC conjugate (Santa Cruz, USA). Finally, the parasites and sections were washed for three times with PBS, and observed with a fluorescent microscope (Olympus, Japan) [43 (link)].
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