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2 protocols using dichloromethane

1

Phthalate Standards Quantification Protocol

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Solvents used for this work include high performance liquid chromatography (HPLC) grades of hexane, methanol, acetone, ultrapure water and dichloromethane (Waters, Milford, MA, USA). Phthalate standards included di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethyl hexyl phthalate (DEHP), di-n-octyl Phthalate (DnOP), di-isononyl phthalate (DiNP), diisodecyl phthalate (DIDP) (AccuStandard Inc, (New Haven, CT, USA). Internal standard solutions, including phenanthrene-d10 and chrysene-d12 and standard surrogate solutions, 2-fluorobiphenyl and 4-terphenyl-d14, were obtained SUPELCO Inc. (Bellefonte, PA, USA).
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2

Polycyclic Aromatic Hydrocarbon Analysis

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Reagents mainly included anhydrous sodium sulfate (purchased from Chengdu Kelong Chemical Reagent Factory, Chengdu, China) and dichloromethane at chromatographic-grade purity (provided by Waters Company, Milford, MA, USA). The 16PAHs in a standard (made by AccuStandard Inc., New Haven, CT, USA) were Naphthalene (NAP), Acenaphthylene (ACY), Acenaphthene (ACE), Fluorene (FLU), Phenanthrene (PHE), Anthracene (ANT), Fluoranthene (FLA), Pyrene (PYR), Benzo(a)Anthracene (BaA), Chrysene (CHR), Benzo(b)Fluoranthene (BbF), Benzo(k)Fluoranthene (BkF), Benzo(a)Pyrene (BaP), Indeno(1,2,3-c,d)Pyrene (InP), Dibenzo(a, h)Anthracene (DBA), and Benzo(g,h,i)perylene (BgP). Extraction kits for QuEChERS were procured from Agilent Technologies Inc. (Santa Clara, CA, USA) and had the following composition: 150 mg C18, 50 mg PSA, and 900 mg Na2SO4.
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