Cys-GGGRDN(M0)-ZHER2:342 was kindly gifted by the Jiangsu Institute of Nuclear Medicine, and its chemical purity was greater than 95%. The maleimide derivative 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA-MAL) was purchased from CheMatech (Dijon, France). 18F was generated from a cyclotron through the proton irradiation of 18O-enriched water (HM67, Sumitomo Heavy Industries). All other commercially obtained chemicals were of analytical grade and used without further purification. The Waters high-performance liquid chromatography (HPLC) system with the Waters 2998 photodiode array detector (PDA) and the preparative C18 HPLC column (5 μm, 250 × 19 mm, Waters 130Xbridge) was used to purify the precursor. Another Waters RP-HPLC system equipped with the Radiometric 610TR flow scintillation analyzer (Perkin Elmer), the Waters 2487 dual λ absorbance detector and the Luna C18 HPLC column (5 μm, 250 × 4.6 mm, Phenomenex) was used to analyze the radiolabeled compounds. The mobile phases A and B were 0.1% v/v trifluoroacetic acid in water and 0.1% v/v trifluoroacetic acid in acetonitrile, respectively.
Luna c18 hplc column
Manufactured by Phenomenex
Sourced in United States
The Luna C18 HPLC column is a reversed-phase chromatography column designed for the separation and analysis of a wide range of organic compounds. It features a silica-based stationary phase with octadecyl (C18) functional groups. The column provides effective separation and high resolution for diverse analytes.
Automatically generated - may contain errors
Lab products found in correlation
Nicolet is50 ftir spectrometer, by Thermo Fisher Scientific (2 mentions)
Diaion hp 20, by Merck Group (2 mentions)
Luna phenyl hexyl column, by Phenomenex (2 mentions)
High performance liquid chromatography system, by Waters Corporation (1 mentions)
Waters 2998 photodiode array detector pda, by Waters Corporation (1 mentions)
Sep pak c18, by Waters Corporation (1 mentions)
Radio hplc, by Waters Corporation (1 mentions)
Autopal 4 automatic polarimeter, by Rudolph Research Analytical (1 mentions)
Uv 1800 spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Q tof ultima esi tof mass spectrometer, by Agilent Technologies (1 mentions)
Unity inova 500, by Bruker (1 mentions)
Wheat arabinoxylan, by Megazyme (1 mentions)
1260 lc system, by Agilent Technologies (1 mentions)
6530 accurate mass q tof spectrometer, by Agilent Technologies (1 mentions)
Masshunter software, by Agilent Technologies (1 mentions)
Uv 1800 spectrophotometer, by Shimadzu (1 mentions)
Ultimate 3000 lc system, by Phenomenex (1 mentions)
Cto 10as vp, by Shimadzu (1 mentions)
Varian prostar 410 autosampler, by Agilent Technologies (1 mentions)
7 protocols using luna c18 hplc column
1
Radiolabeling of Cys-GGGRDN(M^0)-ZHER2:342
2
Radiolabeling of IR808-DOTA with Gallium-68
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In brief, 10 μL of IR808-DOTA (1 μg/μL, dissolved
in ultrapure water) was mixed with 250 μL of sodium acetate
buffer (0.25 M). Then, radiolabeling was accomplished by the addition
of 1 mL of 68GaCl3 (0.05 M hydrochloric acid
as eluant, 100∼200 MBq) obtained from a commercial 68Ge/68Ga generator (Isotope Technologies Garching GmbH,
Garching, Germany). After heat shock at 95 °C for 10 min, the
mixture was purified by Sep-Pak C-18 (Waters Corporation, Milford,
MA, USA). Free 68Ga was diluted with 5 mL of deionized
water, and the desired 68Ga-DOTA-IR808 was eluted with
500 μL of 95% ethanol. Radiochemical purity and yield were tested
using radio high-performance liquid chromatography (radio-HPLC, Waters
Corporation) equipped with a 1525 Binary Pump, a 2489 UV/visible detector,
an FC-3200 flow count radiation detector (Eckert & Ziegler, Germany),
and a 4.6 × 250 mm Luna C18 HPLC column (Phenomenex, CA, USA).
The solvent system was a gradient of H2O with 0.1% TFA
(A) and acetonitrile (B): flow rate 1.0 mL/min; 0–5 min 95%
A/5% B; 5–10 min 95% A/5% B to 5% A/95% B; 10–15 min
5% A/95% B; 15–20 min 5% A/95% B to 95% A/5% B.
in ultrapure water) was mixed with 250 μL of sodium acetate
buffer (0.25 M). Then, radiolabeling was accomplished by the addition
of 1 mL of 68GaCl3 (0.05 M hydrochloric acid
as eluant, 100∼200 MBq) obtained from a commercial 68Ge/68Ga generator (Isotope Technologies Garching GmbH,
Garching, Germany). After heat shock at 95 °C for 10 min, the
mixture was purified by Sep-Pak C-18 (Waters Corporation, Milford,
MA, USA). Free 68Ga was diluted with 5 mL of deionized
water, and the desired 68Ga-DOTA-IR808 was eluted with
500 μL of 95% ethanol. Radiochemical purity and yield were tested
using radio high-performance liquid chromatography (radio-HPLC, Waters
Corporation) equipped with a 1525 Binary Pump, a 2489 UV/visible detector,
an FC-3200 flow count radiation detector (Eckert & Ziegler, Germany),
and a 4.6 × 250 mm Luna C18 HPLC column (Phenomenex, CA, USA).
The solvent system was a gradient of H2O with 0.1% TFA
(A) and acetonitrile (B): flow rate 1.0 mL/min; 0–5 min 95%
A/5% B; 5–10 min 95% A/5% B to 5% A/95% B; 10–15 min
5% A/95% B; 15–20 min 5% A/95% B to 95% A/5% B.
3
Analytical Techniques for Compound Characterization
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Optical rotations were measured with a Rudolph Research Analytical Autopal IV Automatic polarimeter. UV and IR spectra were obtained with Shimadzu UV-1800 spectrophotometer and Thermo scientific Nicolet iS50FT-IR spectrometer, respectively. NMR spectra were recorded in methanol-d4 on Varian Unity Inova 500 MHz and Bruker 400 MHz. High resolution mass spectra were obtained an Agilent Q-TOF Ultima ESI-TOF mass spectrometer. HPLC was carried out on Agilent 1100 LC system using a Phenomenex Luna C18 column (100 mm × 21.2 mm, 5 μm particle size), a Phenomenex Luna phenyl-hexyl column (250 mm × 10 mm, 5 μm particle size) and a Phenomenex Luna C18 HPLC column (250 mm × 10 mm, 5 μm particle size). Column chromatography used Diaion HP-20 (Sigma).
4
Carbohydrate Characterization by HPLC
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AMAC was from Fluka, tamarind xyloglucan was a generous gift from Dr K. Yamatoya (Dainippon Pharmaceutical Co.), wheat arabinoxylan was from Megazyme, and other carbohydrates were from Sigma–Aldrich. Galacturonobiose, -triose and -tetraose were prepared from homogalacturonan by partial digestion with endo-polygalacturonase and purified by gel-permeation chromatography on Bio-Gel P-2.
The Luna C18 HPLC column [250 mm×4.6 mm, 5 μm C18(2) silica 100 Å] was from Phenomenex. The HPLC eluents were from VWR or Fisher Scientific.
The Luna C18 HPLC column [250 mm×4.6 mm, 5 μm C18(2) silica 100 Å] was from Phenomenex. The HPLC eluents were from VWR or Fisher Scientific.
5
Accurate-Mass Q-TOF Analysis of Samples
6
Characterization of Organic Compounds
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Optical rotation was measured with a Rudolph Research Analytical AutoPol IV Automatic Polarimeter. UV and IR spectra were obtained with Shimadzu UV-1800 spectrophotometer (Shimadzu, Kyoto, Japan) and Thermo scientific Nicolet iS50FT-IR spectrometer (Thermo Fisher Scientific, Waltham, MA, USA), respectively. CD spectra were recorded on Jasco J-815 circular dichroism spectrophotometer (Jasco Products Company, Oklahoma City, OK, USA) in methanol at the concentration of 0.01 mg/mL (the length of the cell path was 1 cm). NMR spectra including 1D and 2D experiments were recorded on a Bruker 400 MHz NMR; HPLC was carried out on Thermo scientific Ultimate 3000 LC system using a Phenomenex Luna phenyl-hexyl column (100 mm × 21.2 mm, 5 μm particle size, Phenomenex, Torrance, CA, USA) and a Phenomenex Luna C18 HPLC column (250 mm × 10 mm, 5 μm particle size, Phenomenex, Torrance, CA, USA). All solvents were HPLC grade. Column chromatography was performed using Diaion HP-20 (Sigma, St. Louis, MO, USA).
7
HPLC Validation for Drug Release Studies
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The PTX concentrations for the drug release studies were determined using an HPLC method validated in our laboratory previously [71 (link)]. The analyses were made using Phenomenex Luna C-18 HPLC column (25 cm × 4.6 mm; particle size 5 μm) (Phenomenex, Torrance, CA, USA), combined with Phenomenex C-18 (4 mm × 3 mm) pre-column (Phenomenex, Torrance, CA, USA). The analyses were performed using Dionex apparatus comprising HPLC pump model 7580, DAD detector UVD 340S (Dionex, Sunnyvale, CA, USA) and Jetstream II Plus (WO Industrial Electronics, Vienna, Austria) thermostat. The other used HPLC system was combined of Varian ProStar 210 pump, Varian ProStar 410 autosampler, Varian ProStar 325 UV-Vis detector (Varian, Palo Alto, CA, USA) and Shimadzu CTO-10ASvp (Shimadzu, Kyoto, Japan) thermostat. The analyses were made in isocratic conditions; the mobile phase was a mixture of acetonitrile, methanol and water (60:2:38 (v/v/v) with 0.1% of trifluoroacetic acid. The flow was set as 1.00 mL/min, the column temperature was 30 °C and the injection volume was 20 μL. The chromatograms were recorded at λ = 229 nm.
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