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Diphenylhexatriene dph

Manufactured by Merck Group
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Sourced in Spain

Diphenylhexatriene (DPH) is a fluorescent probe used in laboratory settings. It is a hydrophobic molecule that partitions into lipid bilayers and can be used to study the fluidity and dynamics of cellular membranes.

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Lab products found in correlation

Tetracycline, by Merck Group (1 mentions) Live dead baclight bacterial viability kit, by Thermo Fisher Scientific (1 mentions) Amikacin, by Merck Group (1 mentions) Linezolid, by Merck Group (1 mentions) Acridine orange, by Merck Group (1 mentions) Phenyl arginyl β naphthylamide, by Merck Group (1 mentions) Deuterium oxide, by Merck Group (1 mentions) Haba avidin reagent, by Merck Group (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Pluronic f 127, by Merck Group (1 mentions) Pyrene, by Merck Group (1 mentions) Fluorescamine, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) 1 4 dioxane, by Merck Group (1 mentions) Oleic acid, by Cayman Chemical (1 mentions) Eicosapentaenoic acid, by Cayman Chemical (1 mentions) Docosahexaenoic acid, by Cayman Chemical (1 mentions) Egg phosphatidylcholine, by Avanti Polar Lipids (1 mentions)

Close spelling variants of this product

1,6-Diphenylhexatriene (DPH Diphenylhexatriene

3 protocols using diphenylhexatriene dph

1

Colistin Antibiotic Resistance Assay

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Colistin was kindly supplied by Zhejiang Shenghua Biok Biology Co., Ltd., (Shanghai, China), Chloramphenicol, amikacin, tetracycline, linezolid, acridine orange, the efflux pump inhibitor phenyl-arginyl-β-naphthylamide (PaβN), used in the accumulation assays, and the fluorescent probes diphenylhexatriene (DPH) and 1-[4-trimethylamino-phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH) were purchased from Sigma-Aldrich Chemicals (Madrid, Spain). The LIVE/DEAD® BacLight™ bacterial viability kit was from Life Technologies (Oregon, USA).
Armengol E., Domenech O., Fusté E., Pérez-Guillén I., Borrell J., Sierra J, & Vinas M. (2019). Efficacy of combinations of colistin with other antimicrobials involves membrane fluidity and efflux machinery. Infection and Drug Resistance, 12, 2031-2038.
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2

Synthesis and Characterization of Multifunctional Nanocarriers

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1-ethyl-3-carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), succinic anhydride, 4-(dimethylamino)pyridine (DMAP), N,N-diisopropylethylamine (DIPEA), triethylamine (TEA), N,N′-dicyclohexylcarbodiimide (DCC), dimethyl sulfoxide (DMSO), 1,4-dioxane, dichloromethane, deuterium oxide (D2O), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), phosphate-buffered saline (PBS), and Milex-GP syringe filter (0.22 μm, poly(ether sulfone), 33 mm) were purchased from Merck. Pluronic® F127, biotin (BT), spermine (SN), folic acid (FA), HABA/Avidin reagent, fluorescamine, diphenylhexatriene (DPH), and pyrene were purchased from Sigma-Aldrich. RPMI-1640 medium, L-glutamine, and fetal bovine serum (FBS) were obtained from Invitrogen. HY was synthesized as previously reported [46 (link)].
de Morais F.A., De Oliveira A.C., Balbinot R.B., Lazarin-Bidóia D., Ueda-Nakamura T., de Oliveira Silva S., da Silva Souza Campanholi K., da Silva Junior R.C., Gonçalves R.S., Caetano W, & Nakamura C.V. (2022). Multifunctional Nanoparticles as High-Efficient Targeted Hypericin System for Theranostic Melanoma. Polymers, 15(1), 179.
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3

Lipid Membrane Composition Analysis

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Chloroform, dichloromethane and methanol, HPLC-grade solvents, were obtained from Merck (Darmstadt, Germany). Egg phosphatidylcholine (PC), egg sphingomyelin (SM), 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) and 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (PDPC) were obtained from Avanti Polar Lipids (Birmingham, AL, USA). Diphenyl hexatriene (DPH) was supplied by Sigma-Aldrich (Madrid, Spain). Docosahexaenoic acid, eicosapentaenoic acid and oleic acid were provided by Cayman Chemical (Ann Arbor, MI).
De Santis A., Varela Y., Sot J., D’Errico G., Goñi F.M, & Alonso A. (2018). Omega-3 polyunsaturated fatty acids do not fluidify bilayers in the liquid-crystalline state. Scientific Reports, 8, 16240.
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