Coptisine

Manufactured by Yuanye Bio-Technology

Sourced in China

Coptisine is a biochemical compound extracted from the roots of the Coptis plant. It functions as a bioactive compound with applications in various research and analytical settings.

Automatically generated - may contain errors

Lab products found in correlation

6 protocols using coptisine

Investigating Antioxidant Compounds in Cell Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cyanidin 3−sambubioside (C3S; CAS no. 33012−73−6), luteolin−5−O−glucoside (L5G; CAS no. 20344−46−1), rutin (RUT; CAS no. 153−18−4), echinacoside (ECH; CAS no. 82854−37−3), apigenin (API; CAS no. 520−36−5), kaempferol (KAEM; 520−18−3), coptisine (COP; CAS no. 3486−66−6), magnoflorine (MAG; CAS no. 2141−09−5), and piperine (PIP; CAS no. 94−62−2) were purchased from Yuanye Biotechnology Company (Shanghai, China). α−Tocopherol (αT; CAS no. 10191−41−0), ellagic acid (EA; CAS no. 476−66−4), and H₂O₂ were obtained from Aladdin® (Shanghai, China). RPMI−1640 medium and fetal bovine serum were procured from Hyclone (Logan, Utah, USA), 0.5% trypsin EDTA, penicillin, and streptomycin were purchased from Keyi (Hangzhou, China). Antibodies to Nrf2, histone H3, and β−actin; anti−mouse−horseradish peroxide (HRP) IgG; and anti−rabbit–HRP–IgG were acquired from Abcam (London, UK). α−Tocopherol was used as a positive control.

Li M., Huang W., Jie F., Wang M., Zhong Y., Chen Q, & Lu B. (2019). Discovery of Keap1−Nrf2 small−molecule inhibitors from phytochemicals based on molecular docking. Food and Chemical Toxicology, 133, 110758.

+ Open protocol

+ Expand

BTWT Herbal Components and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The concentrated granules (with a concentrate to dried herb ratio of 10:0.9) of four component herbs of BTWT, R. pulsatilla, R. coptidis, C. phellodendri, and C. fraxini (lot numbers 8055671, 9010401, 1612619, and 7122571, respectively), were purchased from Guangdong Yifang Pharmaceutical Co., Ltd (Foshan, China). The structures of representative components from the four herbs are shown in Fig. 1. AB4, AA3, 23-HA, aesculin, aesculetin, palmatine, jatrorrhizine, coptisine, epiberberine, and phellodendrine were purchased from Yuanye Bio-Technology Co., Ltd at the highest available purity (98%) (Shanghai, China). Berberine hydrochloride was purchased from Shenzhen ChemStrong Scientific Co., Ltd (Shenzhen, China) at the highest available purity (95%). DSS was purchased from MP Biomedicals (MW; 36,000 − 50,000, Solon, OH, USA). The stool bleeding test kit was purchased from BaSO Bio-Technology Co., Ltd (Zhuhai, China). Lipopolysaccharide (LPS) was purchased from Sigma-Aldrich. Deionized water was purified using a Millipore water purification system (Millipore, Milford, MA, USA). All other reagents used were of analytical grade.Fig. 1

The label components of the four component herbs of BTWT

Deng L.R., Han Q., Zou M., Chen F.J., Huang C.Y., Zhong Y.M., Wu Q.Y., Tomlinson B, & Li Y.H. (2022). Identification of potential immunomodulators from Pulsatilla decoction that act on therapeutic targets for ulcerative colitis based on pharmacological activity, absorbed ingredients, and in-silico molecular docking. Chinese Medicine, 17, 132.

+ Open protocol

+ Expand

Composition and Characterization of TAZF

Check if the same lab product or an alternative is used in the 5 most similar protocols

Carbamazepine (CAS No.: 298–46-4), berberine (CAS No.: 2086-83-1), coptisine (CAS No.: 6020-18-4), epiberberine (CAS No.: 6873-09-2), palmatine (CAS No.: 3486-67-7), jatrorrhizine (CAS No.: 3621-38-3), columbamine (CAS No.: 483–34-1), evodiamine (CAS No.: 518–17-2) and rutaecarpine (CAS No.: 20575–76-2), all > 98% purity, were purchased from Shanghai Yuanye Bio-Technology Company. Acetonitrile and methanol (LC grade) were purchased from Fisher Chemical, USA. Formaldehyde (AR) was purchased from Beijing Tong Guang Fine Chemicals Company. Isoflurane was provided by HARVEYBIO Company.
TAZF was provided by the State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University. The concentrations of the compounds in TAZF were measured using high-performance liquid chromatography (HPLC), with berberine accounting for the majority of TAZF at a ratio of 14.73% (g/g), followed by palmatine (3.14%), coptisine (2.52%), evodiamine (1.41%), epiberberine (1.30%), rutaecarpine (1.27%), columbamine (0.90%) and jatrorrhizine (0.47%).

Wang S., Zhang T., Liu X., Yang Z., Li L., Shan D., Gao Y., Li Y., Li Y., Zhang Y, & Wang Q. (2022). Toxicity and toxicokinetics of the ethanol extract of Zuojin formula. BMC Complementary Medicine and Therapies, 22, 220.

+ Open protocol

+ Expand

Herbal Compound Evaluation for Inflammatory Bowel Disease

Check if the same lab product or an alternative is used in the 5 most similar protocols

XLP was purchased from the Hubei Xianglian Pharmaceutical Co., Ltd. (Z42020398, Hubei, China, the compositions were shown in Table 1). Standard solutions of berberine, jatrorrhizine, costunolide, coptisine, and palmatine were purchased from Yuanye Bio-Technology Co., Ltd (Shanghai, China, cat# B21449, B21451, B20891, B21438, B21433). DSS (36,000–50,000 MW) was purchased from MP Biomedicals (Santa Ana, CA, United States, cat# 160110). 5-aminosalicylic acid (5-ASA) was purchased from Sigma-Aldrich (St. Louis, MO, United States, cat#A79809). 3-methyladenine (3-MA) was purchased from Selleck (Huston, TX, United States, cat#S2767).

Wang B., Gong Z., Zhan J., Yang L., Zhou Q, & Yuan X. (2021). Xianglian Pill Suppresses Inflammation and Protects Intestinal Epithelial Barrier by Promoting Autophagy in DSS Induced Ulcerative Colitis Mice. Frontiers in Pharmacology, 11, 594847.

+ Open protocol

+ Expand

Optimized Acetylcholinesterase Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Acetylcholinesterase from Electrophorus electricus (eelAChE) type VI-S, acetylthiocholine iodide (ATCh) and 5,5 -dithio-bis-(2nitrobenzoic acid) (DTNB or Ellman's reagent) were purchased from Sigma-Aldrich (Shanghai, China). 3-(trimethoxysilyl)propyl methacrylate (␥-MAPS), 2,2 -azobisisobutyronitrile (AIBN), glycidyl methacrylate (GMA), ethylene dimethacrylate (EDMA), galantamine, 1,4-butanediol, 1-propanol and ammonium acetate were all purchased from Aladdin Chemicals (Shanghai, China). Acebutolol hydrochloride was obtained from the Guangdong Institute for Food and Drug Control (Guangzhou, China). Coptisine, berberine, jatrorrhizine hydrochloride, palmatine hydrochloride, columbamine, dehydrocorydaline, tetrahydropalmatine and corydaline were purchased from Shanghai Yuanye Bio-Technology Co. Ltd. HPLC-grade methanol (MeOH) and acetonitrile (ACN) were obtained from Merck (Shanghai, China). The fused-silica capillaries with an inner diameter of 400 m (800 m O.D.) were purchased from Polymicro Technologies Ltd. (Phoenix, AZ, USA).

Wang L., Zhao Y., Zhang Y., Zhang T., Kool J., Somsen G.W., Wang Q, & Jiang Z. (2018). Online screening of acetylcholinesterase inhibitors in natural products using monolith-based immobilized capillary enzyme reactors combined with liquid chromatography-mass spectrometry. Journal of chromatography. A, 1563.

+ Open protocol

+ Expand

Protective Effects of HDAX on ER Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols

HDAX were mainly composed of Coptidis Rhizoma, Radix Rehmanniae, Radix Puerariae, Radix Ophiopogonis, Eriobotryae Folium, and Radix Notoginseng, which were purchased from Anhui Provincial Hospital of Chinese Medicine. Notoginsenoside R1, ginsenoside Rg1, puerarin, acteoside, berberine, coptisine, and palmatine hydrochloride were purchased from Shanghai yuanye Bio-Technology Co., Ltd (Shanghai, China). 4-phenylbutyric acid (4-PBA) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The primary antibody of GRP78, CHOP was purchased from A nity Bioscience (1:1300, USA); Caspase-12, Caspase-9, Caspase-3 were obtained from Bioss (1:1000, Beijing, China); Bax, Bcl-2 was provided by Bioworld (1:1000, Nanjing, China).

Ye T., Xuan W., Zhou P., Shao N., Song H., Wang Y., Hu G., Wang L., Wang G., Fang Z., & Ye T. (2021). Huangdi Anxiao Attenuated High Glucose-Induced PC12 Cells Neurotoxicity via Inhibiting Apoptosis Pathway of Endoplasmic Reticulum Stress.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!