Human DP cells (PromoCell, Heidelberg, Germany) were maintained in follicle DP cell growth medium (DPCGM) (PromoCell), which was changed every 2–3 days. Cells from the fourth passage were used in the experiments. HUVECs (Angio-proteomie, Boston, MA, USA) and GFP/RFP-induced HUVECs (GFP/RFP-HUVEC, Angio-proteomie) were maintained in endothelial cell growth medium (EGM-2; Lonza, Basel, Switzerland), which was refreshed every 2–3 days. Cells from passages 5–7 were used in the experiments. Mixed culture medium, composed of DPCGM, KG2, and EGM-2 (1:1:1 ratio), was used for HFG culture in the HFG chips.
Human dp cells
Manufactured by PromoCell
Sourced in Germany, United States
Human DP cells are primary dermal papilla cells isolated from human tissue. They are a type of specialized cells found in the hair follicle. The core function of human DP cells is to provide structural and signaling support for hair follicle growth and development.
Automatically generated - may contain errors
2 protocols using human dp cells
1
Maintenance of Human Dermal Papilla Cells
2
Cytotoxicity Evaluation of RE-ORGA
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Human dermal fibroblasts (ATCC, Manassas, VA, USA) and human DP cells (Promocell, Heidelberg, Germany) were seeded in 96-well plates at a density of 10,000 cells/well and incubated in a humidified atmosphere with air and 5% CO2 at 37℃. These cultured cells were then treated with various concentrations of RE-ORGA for 24 hours. Cell viability was determined by assessing the ability of cells to cleave tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) to formazan. Optical densities were measured at a wavelength of 570 nm on a SpectraMax 340PC384 Absorbance Microplate Reader (Molecular Devices, LLC., San Jose, CA, USA).
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