Western blot analysis was performed as described previously36 (link). The primary antibodies were as follows: anti-p27 (ab193379, Abcam), anti-phosphorylated Rb (Ser795) (#9301, Cell Signaling Technology), anti-E2F1 (#3742, Cell Signaling Technology), anti-p21 (#2947, Cell Signaling Technology), anti-caspase-3 antibody (ab32351, Epitomics), anti-FHL2 (ab12327, Abcam), anti-β-catenin (#8480, Cell Signaling Technology), anti-phosphorylated β-catenin (Ser33/37/Thr41) (#9561, Cell Signaling Technology), anti-cyclin D1 (60186-lg, Proteintech), anti-p53 (10442-1-AP, Proteintech), anti-PUMA (ab33906, Epitomics), anti-E-cadherin (20874-1-AP, Proteintech), anti-N-cadherin (22018-1-AP, Proteintech), and anti-GAPDH (#2118, Cell Signaling Technology).
Anti cyclin d1 60186 lg
Manufactured by Proteintech
Sourced in United States
Anti-cyclin D1 (60186-1-Ig) is a primary antibody product offered by Proteintech. It is a polyclonal antibody that specifically recognizes the cyclin D1 protein. Cyclin D1 is a key regulator of the cell cycle, playing a crucial role in the progression from the G1 to the S phase. The primary function of this antibody is to detect and study the expression and localization of cyclin D1 in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Ab193379, by Abcam (2 mentions)
Anti phosphorylated rb ser795, by Cell Signaling Technology (2 mentions)
Anti p21, by Cell Signaling Technology (2 mentions)
Anti gapdh, by Cell Signaling Technology (2 mentions)
Ab32351, by Abcam (1 mentions)
Ab12327, by Abcam (1 mentions)
Anti e2f1, by Cell Signaling Technology (1 mentions)
Anti β catenin, by Cell Signaling Technology (1 mentions)
Anti n cadherin 22018 1 ap, by Proteintech (1 mentions)
Anti phosphorylated β catenin ser33 37 thr41, by Cell Signaling Technology (1 mentions)
Anti e cadherin 20874 1 ap, by Proteintech (1 mentions)
Anti p53, by Proteintech (1 mentions)
Ab14601, by Abcam (1 mentions)
Anti irs1, by Cell Signaling Technology (1 mentions)
Anti akt 10176 2 ap, by Proteintech (1 mentions)
Anti p27, by Abcam (1 mentions)
2 protocols using anti cyclin d1 60186 lg
1
Western Blot Analysis of Cell Signaling Proteins
2
Comprehensive Western Blot Analysis Protocol
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Western blot analysis was performed as described previously40 (link). The following primary antibodies were used: anti-cyclin D1 (60186-lg, Proteintech, Chicago, IL, USA), anti-p27 (ab193379, Abcam, Cambridge, UK), anti-p21 (#2947, Cell Signaling Technology, Danvers, MA, USA), anti-phosphorylated Rb (Ser795) (#9301, Cell Signaling Technology), anti-IRS1 (#2382, Cell Signaling Technology), anti-phosphorylated IRS1 (Ser307) (D151214, BBI, Shanghai, China), anti-IGF1R (20254-1-AP, Proteintech), anti-phosphorylated AKT (Ser473) (66444-1-lg, Proteintech), anti-AKT (10176-2-AP, Proteintech), anti-phosphorylated mTOR (Ser2448) (D155324, BBI), anti-mTOR (20657-1-AP, Proteintech), anti-FoxP3 (D260367, BBI), anti-Dicer (ab14601, Abcam), anti-Drosha (55001-1-AP, Proteintech), and anti-GAPDH (#2118, Cell Signaling Technology).
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