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2 protocols using 2 bromoisobutyryl bromide

1

Synthesis of Fluorescent Polymeric Nanoparticles

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MPC was purchased from Sigma-Aldrich Japan Co., LLC. (Tokyo, Japan). BMA, pentaerythritol, and triethylamine were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan), while 2-Bromoisobutyryl bromide was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Ethylene bis(2-bromobutyrate), 2,2′-bipyridyl, copper(II) bromide, and l-ascorbic acid were purchased from Sigma-Aldrich Japan Co., LLC. (Tokyo, Japan). HeLa cells were obtained from the RIKEN Cell Bank (Saitama, Japan). RPMI-1640, Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), and phosphate-buffered saline (PBS) were purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Cell counting kit-8 (CCK8) was purchased from Dojindo Laboratories Co., Ltd. (Kumamoto, Japan), LDH-Cytotoxic Test Wako from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan), Spectra/Por dialysis membrane from Spectrum Chemical Mfg. Corp. (New Brunswick, NJ, USA), and Methacryloxyethyl thiocarbamoyl rhodamine B from Polysciences Inc. (Warrington, PA, USA). The other solvents and reagents were of extra-pure grade and used without further purification.
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2

Synthesis and Characterization of Doxorubicin-Loaded Stimuli-Responsive Polymeric Nanoparticles

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Doxorubicin hydrochloride (DOX⋅HCl) was purchased from Beijing Huafeng United Technology Co., Ltd. Lactobionic acid, 2-aminoethyl methacrylate hydrochloride, and N,N′-bis(acryloyl)cystamine (BAC) were purchased from Sigma-Aldrich. N-isopropylacrylamide (NIPAM; Sinopharm Chemical Reagent Co., Ltd) was recrystallized from an n-hexane/toluene mixture. CuBr (Tianjin, People’s Republic of China, analytical reagent) was purified by dissolving in concentrated HCl, precipitating in water, washing with ethanol and ethyl ether three times, and then drying under vacuum. The following reagents were of analytic grade and used as received: 2-bromoisobutyryl bromide (Tokyo Chemical Industry), dithiothreitol (Acros Organics), 2,2′-bipyridine (bpy 99%, Sigma-Aldrich), and triethylamine (Sigma-Aldrich). 2-Lactobionamidoethyl methacrylamide (LAMA) was synthesized using a method described elsewhere.27 (link),28 (link) Water was purified by a Milli-Q system (Millipore Corporation) to a specific resistivity of ~18 MΩ cm. All solvents were distilled and dried by storing over activated 4Å molecular sieves for 24 h prior to use.
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