The following chemicals were used: 2c [9 (link)], dimethyl sulfoxide (DMSO), Doxorubicin, MK2206, Torin1, Bafilomycin A1, Chloroquine, ABT263 (Sigma), Gemcitabine, XMD8-92, YKL-06-061, ABT199 (CSN Scientific), Selumetinib, TMP195, MKC3946 (MedChemExpress), SAHA (Cayman Chemicals), NKL54 [46 (link)].
Abt 263
Manufactured by Merck Group
Sourced in United States
ABT-263 is a lab equipment product offered by Merck Group. It is a small molecule that functions as a selective and potent inhibitor of the apoptosis regulator Bcl-2.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Selumetinib, by MedChemExpress (1 mentions)
Mkc3946, by MedChemExpress (1 mentions)
Danazol, by Merck Group (1 mentions)
Quercetin, by Avantor (1 mentions)
Nicotinamide riboside, by Merck Group (1 mentions)
Abt 263, by Selleck Chemicals (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Nicotinamide riboside, by ChromaDex (1 mentions)
Gefitinib, by Merck Group (1 mentions)
At 101, by Merck Group (1 mentions)
Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions)
A2780cis, by American Type Culture Collection (1 mentions)
A2780, by American Type Culture Collection (1 mentions)
A2780, by Merck Group (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
5 protocols using abt 263
1
Multi-Compound Cytotoxicity Screening
2
Evaluation of Compound Treatments on Cells
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Cells were treated either with ABT-263 (Selleck Chemicals, Houston, TX, USA), quercetin (Sigma-Aldrich, St. Louis, MO, USA), nicotinamide riboside (ChromaDex, Irvine, CA, USA), or danazol (Sigma-Aldrich). These compounds were dissolved in DMSO (VWR, Radnor, PA, USA) (ABT-263), ethanol (VWR) (quercetin and danazol), or phosphate-buffered saline (Sigma-Aldrich) (nicotinamide riboside), according to the manufacturer’s instructions, and subsequently diluted in cell culture medium to working concentrations of 10 μM (ABT-263), 100 μM (quercetin and danazol), or 20 mM (nicotinamide riboside). If not stated otherwise, the compounds were applied only for 3 days.
3
Gefitinib-Resistant Cell Culture and Reagents
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PC-9 gefitinib-resistant cells (PC-9-GR) was kindly provided by Dr. Bing Xia at Hangzhou Cancer Hospital (Hangzhou, China) [16 (link)]. H1975 cell lines was obtained from the American Type Culture Collection (Manassas, VA, USA). Cells were cultured in standard RPMI-1640 media (Invitrogen, Carlsbad, CA, USA) supplemented with 10 % heat-inactivated fetal bovine serum (Invitrogen, Carlsbad, CA, USA), 2 mM l-glutamine, and 1 % penicillin/streptomycin at 37 °C in a humidified incubator with 5 % CO2. AT-101, ABT-263, and gefitinib were purchased from Sigma (St. Louis, MO, USA). Both reagents were dissolved in dimethyl sulfoxide (DMSO), stored at−80 °C, and diluted in culture medium for described experiments.
4
Mitochondrial Dynamics in Cancer Cell Lines
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Cell lines were selected to give a broad overview of multiple cancer types, including breast, ovarian, pancreatic, and hematologic cancers. All cell lines except A2780, A2780-cis and patient derived lines were obtained from ATCC and cultured as directed. Patient-derived cell lines were obtained and cultured as previously described28 (link). Briefly, patient-derived cells were obtained from ovarian cancer patients and were cultured under identical conditions as described28 (link). The A2780 and cisplatin-resistant A2780 cell lines were obtained from Sigma-Aldrich. These cell lines were transduced with a mitochondrial targeted-GFP using a lentiviral construct consisting of mito-PAGFP (Addgene Plasmid #23348), where the mitochondrial targeting sequence and GFP were cut and inserted into a pLVX-AcGFP1-C1 Vector backbone (Clonetech #632155). Lentivirus production was completed following a standard protocol29 (link). cisplatin and ABT-263 were obtained from Sigma-Aldrich and dissolved in DMF and DMSO, respectively, prior to cell treatment.
5
Renal Cell Carcinoma Cell Culture
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Human RCC cells (786-O and Caki-1) were obtained from Shanghai Bank for Cell Culture (Shanghai, China). All cells were cultured in RPMI-1640 medium (Life Technologies, USA) supplemented with 10% FBS (Gibco, USA). Cells were maintained in humidified atmosphere with 5% CO2 at 37°C. TW-37, ABT-263 and all other chemicals were obtained from Sigma-Aldrich (St Louis, USA). TW-37 and ABT-263 were dissolved in DMSO at a stock solution of 100 mM and diluted with FBS-free medium to achieve desired concentrations.
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