Paraffin sections were stained for elastic fibers using van Gieson Elastic Stain kit according to the manufacturer’s instructions. The section from the largest diameter of AAA for each animal was selected for staining. Briefly, deparaffinized and hydrated sections were stained in elastic stain solution for 30 minutes and decolorize in differentiating solution. Following that sections were rinsed in sodium thiosulfate solution shortly and stained in Van Gieson stain solution for 15 minutes. Sections were viewed with a BX51 upright microscope (Olympus, Japan) using Olympus CellSens Standard acquisition software. The surface area occupied by elastic fibers staining was quantified using image J as described previously.24 (link) Eight visual fields (magnification 200) evenly distributed at media of every lesion section were included to quantify the amount of elastin staining. All images were set to the same hue, saturation and brightness and measured for positive staining area. The elastin content is expressed as a percentage of elastic fiber area in media area.
Cellsens standard acquisition software
Manufactured by Olympus
Sourced in Japan
CellSens Standard acquisition software is a comprehensive imaging and analysis solution for microscopy. It provides tools for image capture, processing, and analysis, enabling users to obtain high-quality data from their experiments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cellsens standard acquisition software
1
Quantifying Aortic Elastin Content
2
Follicle Counting in Pre-Pubertal Female Offspring
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For follicle counting, ovaries of pre-pubertal (PND28) female offspring were collected and fixed overnight in 4% paraformaldehyde (PFA), dehydrated, embedded in paraffin, and sectioned for hematoxylin and eosin staining. Five micrometer-thick sections were serially cut and every 10th section was observed for follicle counting in a blinded manner. Follicles with visible oocyte nuclei were counted. According to the previously described follicle classification (Durlinger et al. 1999 , Detti et al. 2013) (link), the number of follicles at different developmental stages was recorded; specifically, follicles were divided into 5 categories: primordial, primary, secondary, tertiary and atretic follicles. As the number of secondary and tertiary follicles ranges a lot with the follicle cycle selection, we only assess the number of primordial, primary and atretic follicles on PND56. Images were collected on an Olympus DP73 upright microscope (Olympus) with cellSens Standard acquisition software. Micrographs were analyzed by Adobe Photoshop CS6 software (Adobe Systems Inc., San Jose, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!