Trp, 5-hydroxytryptamine (5-HT), kynurenine (Kyn) and quinolinic acid (Quin), and LPS (Escherichia coli, serotype 0111:4) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Benzoyl chloride and ammonium formate were obtained from Aladdin (Shanghai, China). LC-MS grade methanol, acetonitrile, and formic acid were purchased from Fisher Scientific. Enzyme-linked immunosorbent assay (ELISA) kits were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China). NMDAR antibody was purchased from Abcam (Cambridge, UK). The real-time PCR system used in this study was the Thermo Fisher ABI StepOne Plus system. Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis was performed on an Agilent 1290 Infinity II UHPLC system coupled to a 6470A triple quadrupole mass spectrometer (Santa Clara, CA, United States).
Kynurenine kyn
Manufactured by Merck Group
Sourced in United States
Kynurenine (Kyn) is a biochemical compound that plays a role in the metabolism of the amino acid tryptophan. It is a key intermediate in the kynurenine pathway, which is involved in various biological processes. Kynurenine can be measured and quantified using analytical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Abi steponeplus system, by Thermo Fisher Scientific (1 mentions)
1290 infinity 2 uhplc system, by Agilent Technologies (1 mentions)
Lc ms grade methanol, by Thermo Fisher Scientific (1 mentions)
5 hydroxytryptamine 5 ht, by Merck Group (1 mentions)
Quinolinic acid quin, by Merck Group (1 mentions)
6470a triple quadrupole mass spectrometer, by Agilent Technologies (1 mentions)
Interleukin il 17, by Nanjing Jiancheng (1 mentions)
2 protocols using kynurenine kyn
1
Quantification of Neuroactive Metabolites
2
Extraction and Characterization of Citrus Pectin
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Pectin extracted from citrus peel (Henan Yuzhong Biotechnology Co., Ltd., Zhengzhou, China) mainly consisted of galacturonic acid (white powder, with purity of > 81.4%, DM: 13.5%). Microcrystalline cellulose (MCC) is a β-1,4-multi-bonded linear carbohydrate consisting of glucose residues with 99.5% purity. (Beijing NCC Technology R&D Center, China). Reference standards for tryptophan (Trp), tryptamine (Tpm), 3-indoleacetic acid (IAA) and kynurenine (Kyn) were purchased from Sigma-Aldrich (St. Louis, MO, USA); 5-hydroxyindole-3-acetic acid (HIAA) and skatole (ST) were obtained from Cato Research Chemicals Inc. (Eugene, OR, USA); 3-indolylpropionic acid (IPA) and serotonin (5-HT) were from Laboratory of the Government Chemist (Teddington, UK) and Beijing Wokai Biotechnology Co., Ltd. (China), respectively. Assay kits, including interleukin IL-17, IL-22 were purchased from Nanjing Jiancheng Bioengineering Institute (Jiangsu, China).
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