The BM was flushed from the tibia and femur of each mouse. Red blood cells (RBCs) were lysed once for 5 min at 4°C in 0.15 M NH4Cl (StemCell Technologies), washed once with PBS (Gibco), and counted using a cell counter (LUNATM Automated Cell Counter). For the detection of LSK cells and LT-HSCs, Lineage+ cells were removed by magnetic depletion using biotinylated lineage-specific antibodies (CD5, CD45R, CD11b, Gr-1, and Ter-119), followed by depletion with MACs beads conjugated to a monoclonal anti-biotin (Miltenyi Biotec). Lineage− cells were stained with phycoerythrin PE-Cy7-conjugated antibodies to Sca1 (558162), APC-conjugated antibodies to c-kit (553356), FITC-conjugated antibodies to CD48 (557484), and PE-conjugated antibodies to CD150 (561540), all from BD Biosciences. Cells were further stained with streptavidin-pacific blue (PB) (Invitrogen, S11222). Data were collected on a BD AriaIII (BD Biosciences) and analyzed using the FlowJo software (Tree Star).
Fitc conjugated antibodies to cd48 557484
Manufactured by BD
FITC-conjugated antibodies to CD48 (557484) are laboratory reagents used for the detection and analysis of CD48 protein expression on cells. These antibodies are conjugated with the fluorescent dye FITC (Fluorescein Isothiocyanate) to enable visualization and quantification of CD48-positive cells through flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
Pe conjugated antibodies to cd150 561540, by BD (2 mentions)
Streptavidin pacific blue, by Thermo Fisher Scientific (2 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Macs bead, by Miltenyi Biotec (1 mentions)
0.15 m nh4cl, by STEMCELL (1 mentions)
Monoclonal anti biotin, by Miltenyi Biotec (1 mentions)
Aria 3, by BD (1 mentions)
Nh4cl, by STEMCELL (1 mentions)
2 protocols using fitc conjugated antibodies to cd48 557484
1
Multiparameter Characterization of Murine HSCs
2
Isolation and Phenotyping of Hematopoietic Stem Cells
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BM from the tibiae and femurs of each mouse were flushed. Red blood cells (RBCs) were lysed for 5 min at 4 °C in 0.15 M NH4Cl (STEMCELL Technologies, Vancouver, Canada), washed with PBS (Gibco), and counted using a hemocytometer. For HSC, MSC, or osteoblast detection, Lin+ cells were removed by magnetic depletion using biotinylated lineage-specific antibodies (CD5, CD45R, CD11b, Gr-1, and Ter-119), followed by depletion with MACs beads conjugated to monoclonal anti-biotin (Miltenyi Biotec, Gladbach, Germany). For staining of HSCs, Lin− cells were stained with phycoerythrin (PE)-Cy7-conjugated antibodies to Sca1 (558162), APC-conjugated antibodies to c-Kit (553356), FITC-conjugated antibodies to CD48 (557484), and PE-conjugated antibodies to CD150 (561540), all from BD Biosciences. Cells were further stained with streptavidin-pacific blue (PB) (Invitrogen, S11222). Data were collected using AriaIII systems (BD Biosciences) and analyzed using FlowJo software (Tree Star, Ashland, OR).
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