Rabbit polyclonal anti hif 2α

Manufactured by Novus Biologicals

The Rabbit polyclonal anti-HIF-2α is a primary antibody that specifically binds to the Hypoxia Inducible Factor-2 alpha (HIF-2α) protein. It is generated by immunizing rabbits with a recombinant fragment of the HIF-2α protein.

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Lab products found in correlation

Rabbit polyclonal anti v5, by Abcam (2 mentions) Goat anti rabbit hrp, by Thermo Fisher Scientific (2 mentions) Goat anti mouse hrp, by Thermo Fisher Scientific (2 mentions) Mouse monoclonal anti hif 1β, by Novus Biologicals (2 mentions) Mouse monoclonal anti v5 tag, by Thermo Fisher Scientific (2 mentions) Rabbit monoclonal anti hif 2α, by Cell Signaling Technology (2 mentions) Rabbit monoclonal anti hif 1β, by Cell Signaling Technology (2 mentions) Mouse monoclonal anti halotag, by Promega (2 mentions) Mouse monoclonal anti tbp, by Abcam (2 mentions) Anti pcna clone pc10, by Santa Cruz Biotechnology (1 mentions) Peroxidase affinipure goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Rabbit anti mouse igg h l, by Abcam (1 mentions)

Spelling variants of this product

HIF-2α (101 citations) Anti-HIF-2α (30 citations) Rabbit anti-HIF-2α (3 citations)

3 protocols using rabbit polyclonal anti hif 2α

Antibodies for HIF1α and Related Proteins

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We used the following antibodies: mouse monoclonal anti-HIF1α (clone 54, BD Biosciences, 610959); rabbit polyclonal anti-hydroxy-HIF1α-Pro-402 (Millipore, 07-1585); rabbit monoclonal anti-hydroxy-HIF1α-Pro-564 (clone D43B5, 3434), rabbit monoclonal anti-ARNT (clone D28F3, 5537), rabbit monoclonal anti-LSD1 (clone C69G12, 2184), and rabbit polyclonal anti-HDAC1 (2062) from Cell Signaling Technology; rabbit polyclonal anti-HIF2α (Novus Biologicals, NB100-122), mouse monoclonal anti-SREBP1 (clone 2A4, SC-13551), mouse monoclonal anti-Rieske FeS (RISP) (clone A5, SC-271609), and anti-PCNA (clone PC10, #SC-56) from Santa Cruz Biotechnology, Inc.; rabbit anti-thioredoxin (T0803) from Sigma; IRDye 800CW– or IRDye 680RD–conjugated goat anti-mouse or anti-rabbit secondary IgG from LI-COR; Alexa 594–conjugated goat anti-mouse (A11005) IgG from Invitrogen; and peroxidase affiniPure goat anti-rabbit IgG (111-035-144) from Jackson ImmunoResearch Laboratories.

Shao W., Hwang J., Liu C., Mukhopadhyay D., Zhao S., Shen M.C., Selen E.S., Wolfgang M.J., Farber S.A, & Espenshade P.J. (2021). Serum lipoprotein–derived fatty acids regulate hypoxia-inducible factor. The Journal of Biological Chemistry, 295(52), 18284-18300.

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Antibody validation for HIF-1/2α ChIP-seq and Western blot

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The following antibodies were used for ChIP-seq: rabbit polyclonal anti-HIF-2α (Novus Biologicals, Centennial, CO, #NB100-122), mouse monoclonal anti-HIF-1β (Novus Biologicals, #NB100-124), rabbit polyclonal anti-V5 (Abcam, Cambridge, UK, #ab9116). The following antibodies were used for Cut&Run: mouse monoclonal anti-V5 tag (Thermo Fisher, #R960-25) diluted to 0.01 mg/ml, mouse IgG (Jackson ImmunoResearch #015-000-003) diluted to 0.01 mg/ml, rabbit anti-mouse IgG H&L (Abcam, #ab46540) diluted to 0.01 mg/ml. The following antibodies were used for western blotting: rabbit monoclonal anti-HIF-2α (Cell Signaling, Danvers, MA, #D9E3) diluted at 1:1000, rabbit monoclonal anti-HIF-1β (Cell Signaling, #D28F3) diluted at 1:1000, mouse monoclonal anti-V5 tag (Thermo Fisher, #R960-25) diluted at 1:2500, mouse monoclonal anti-HaloTag (Promega, Madison, WI, #G9211) diluted at 1:1000, mouse monoclonal anti-TBP (Abcam, #ab51841) diluted at 1:2500, goat-anti-mouse-HRP (Thermo Fisher, #31430) diluted at 1:2000, goat-anti-rabbit-HRP (Thermo Fisher, #31462) diluted at 1:2000.

Chen Y., Cattoglio C., Dailey G.M., Zhu Q., Tjian R, & Darzacq X. (2022). Mechanisms governing target search and binding dynamics of hypoxia-inducible factors. eLife, 11, e75064.

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Antibodies for ChIP-seq and Western Blotting

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The following antibodies were used for ChIP-seq: rabbit polyclonal anti-HIF-2α (Novus Biologicals, Centennial, CO, #NB100-122), mouse monoclonal anti-HIF-1β (Novus Biologicals, #NB100-124), rabbit polyclonal anti-V5 (Abcam, Cambridge, UK, #ab9116). The following antibodies were used for western blotting: rabbit monoclonal anti-HIF-2α (Cell Signaling, Danvers, MA, #D9E3) diluted at 1:1000, rabbit monoclonal anti-HIF-1β (Cell Signaling, #D28F3) diluted at 1:1000, mouse monoclonal anti-V5 tag (ThermoFisher, # R960-25) diluted at 1:2500, mouse monoclonal anti-HaloTag (Promega, Madison, WI, # G9211) diluted at 1:1000, mouse monoclonal anti-TBP (Abcam, #ab51841) diluted at 1:2500, goat-anti-mouse-HRP (ThermoFisher, #31430) diluted at 1:2000, goat-anti-rabbit-HRP (ThermoFisher, # 31462) diluted at 1:2000.

Chen Y., Cattoglio C., Dailey G.M., Zhu Q., Tjian R., & Darzacq X. (2021). Mechanisms Governing Target Search and Binding Dynamics of Hypoxia-Inducible Factors.

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