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Anti cd45.1 fitc

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Anti-CD45.1-FITC is a fluorescently labeled antibody that binds to the CD45.1 antigen. It is commonly used for cell identification and sorting applications in flow cytometry.

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Lab products found in correlation

Anti cd45 apc efluor 780, by Thermo Fisher Scientific (2 mentions) Anti cd4 pe cy7, by Thermo Fisher Scientific (2 mentions) Cd51 pe, by Thermo Fisher Scientific (2 mentions) Streptavidin apc efluor 780, by Thermo Fisher Scientific (2 mentions) Anti cd31 pe cy7, by Thermo Fisher Scientific (2 mentions) Anti cd150 pe, by BioLegend (2 mentions) Anti cd45.2 pe 104, by BioLegend (2 mentions) Anti cd48 alexa fluor 647 hm48 1 103416, by BioLegend (2 mentions) Anti ter119 apc efluor 780, by Thermo Fisher Scientific (2 mentions) Anti b220 apc efluor 780, by Thermo Fisher Scientific (2 mentions) Anti cd8a pe cy7, by Thermo Fisher Scientific (2 mentions) Anti pdgfrα cd140a apc, by Thermo Fisher Scientific (2 mentions) Anti ly6a e sca 1 fitc, by Thermo Fisher Scientific (2 mentions) Anti cd45 percp cyanine5, by Thermo Fisher Scientific (2 mentions) Anti cd11b mac 1 pe m1 70, by Thermo Fisher Scientific (2 mentions) Lsrii flow cytometer, by BD (1 mentions) Golgiplug, by BD (1 mentions) Anti cd44 fitc, by Thermo Fisher Scientific (1 mentions) Anti ifnγ efluor 450, by Thermo Fisher Scientific (1 mentions) Anti gm csf pe, by BD (1 mentions)

Spelling variants of this product

Anti-CD45 (119 citations) CD45-FITC (92 citations) Anti-CD45-FITC (57 citations) CD45.1 FITC (9 citations)

5 protocols using anti cd45.1 fitc

1

Multi-Omics Analysis of Immune Cells

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Single-cell suspensions of spinal cord, spleen, and inguinal LNs were prepared as previously described [31 (link)]. The following antibodies were used: anti-CD4 PerCP-Cy5.5/APC/eFluor 450/PE-Cy7 (eBioscience, clone RM4-5), anti-CD45.1 FITC (eBioscience, clone A20), anti-CD45.2 PerCP-Cy5.5/APC (eBioscience, clone 104), and anti-CD44 FITC (eBioscience, clone IM7). For intracellular staining, cells were reactivated with culture media (negative control) or 5 μM MOG35−55 peptide for 7 h with GolgiPlug (BD Biosciences) added for the final 4 h. The following intracellular antibodies were used in accordance with the manufacturer’s protocols: anti-IFNγ eFluor 450 (eBioscience, clone XMG1.2), anti-IL17A Alexa Fluor 647 (eBioscience, clone eBio17B7), anti-GM-CSF PE (BD Biosciences, clone MP1-22E9). A viability dye (Aqua, Life Technologies) was applied to exclude dead cells. Samples were acquired by using an LSRII flow cytometer (BD Biosciences) followed by data analysis using FlowJo version 9.x (Tree Star).
McWilliams I.L., Rajbhandari R., Nozell S., Benveniste E, & Harrington L.E. (2015). STAT4 controls GM-CSF production by both Th1 and Th17 cells during EAE. Journal of Neuroinflammation, 12, 128.
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2

Antibody Panel for Mouse Cell Analysis

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The following antibodies were used for flow cytometry: Anti-CD150-PE (TC15–12F12.2; 115904), anti-CD117-PE/Cy7 (2B8; 105814), anti-CD45.2-PE (104; 109807), anti-CD48-Alexa Fluor 647 (HM48–1; 103416) were purchased from BioLegend (San Diego, CA). anti-CD45-APC-eFluor 780 (30-F11; 47–0451-82), anti-Ter119-APC-eFluor 780 (TER-119; 47–5921-82), Streptavidin APC-eFluor 780 (47–4317-82), anti-CD31-PE/Cy7 (MEC13.3; 25–0311-82), anti-CD45.1-FITC (A20; 11–0453-85), anti-B220-APC-eFluor 780 (RA3–6B2; 47–0452-82), anti-CD4-PE/Cy7 (GK1.5; 25–0041-82), anti-CD8a-PE/Cy7 (53–6.7; 25–0081-85), CD51-PE (RMV-7; 13–0512-85), anti-PDGFRα (CD140a)-APC (APA5; 17–1401-81), anti-Ly6A/E(Sca-1)-FITC (D7; 11–5981-82), Anti-CD45-PerCp-Cyanine5.5 (30-F11; 45–0451-82), anti-CD11b (Mac-1)-PE (M1/70; 12–0112-83) were purchased from eBioscience (Thermo Fisher). Anti-lineage panel cocktail (TER-119, RB6–8C5, RA3–6B2, M1/70, 145–2C11 at 1:50 dilution) was from BD Biosciences (559971). Unless otherwise specified, all antibodies were used at a 1:100 dilution. All antibodies were anti-mouse and their specificity was validated in previous studies performed in our laboratory3 (link),8 (link),12 (link),27 (link)–29 (link).
Nakahara F., Borger D.K., Wei Q., Pinho S., Maryanovich M., Zahalka A.H., Suzuki M., Cruz C.D., Wang Z., Xu C., Boulais P.E., Ma'ayan A., Greally J.M, & Frenette P.S. (2019). Engineering a haematopoietic stem cell niche by revitalizing mesenchymal stromal cells. Nature cell biology, 21(5), 560-567.
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3

Antibody Panel for Mouse Cell Analysis

Cited 5 times
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The following antibodies were used for flow cytometry: Anti-CD150-PE (TC15–12F12.2; 115904), anti-CD117-PE/Cy7 (2B8; 105814), anti-CD45.2-PE (104; 109807), anti-CD48-Alexa Fluor 647 (HM48–1; 103416) were purchased from BioLegend (San Diego, CA). anti-CD45-APC-eFluor 780 (30-F11; 47–0451-82), anti-Ter119-APC-eFluor 780 (TER-119; 47–5921-82), Streptavidin APC-eFluor 780 (47–4317-82), anti-CD31-PE/Cy7 (MEC13.3; 25–0311-82), anti-CD45.1-FITC (A20; 11–0453-85), anti-B220-APC-eFluor 780 (RA3–6B2; 47–0452-82), anti-CD4-PE/Cy7 (GK1.5; 25–0041-82), anti-CD8a-PE/Cy7 (53–6.7; 25–0081-85), CD51-PE (RMV-7; 13–0512-85), anti-PDGFRα (CD140a)-APC (APA5; 17–1401-81), anti-Ly6A/E(Sca-1)-FITC (D7; 11–5981-82), Anti-CD45-PerCp-Cyanine5.5 (30-F11; 45–0451-82), anti-CD11b (Mac-1)-PE (M1/70; 12–0112-83) were purchased from eBioscience (Thermo Fisher). Anti-lineage panel cocktail (TER-119, RB6–8C5, RA3–6B2, M1/70, 145–2C11 at 1:50 dilution) was from BD Biosciences (559971). Unless otherwise specified, all antibodies were used at a 1:100 dilution. All antibodies were anti-mouse and their specificity was validated in previous studies performed in our laboratory3 (link),8 (link),12 (link),27 (link)–29 (link).
Nakahara F., Borger D.K., Wei Q., Pinho S., Maryanovich M., Zahalka A.H., Suzuki M., Cruz C.D., Wang Z., Xu C., Boulais P.E., Ma'ayan A., Greally J.M, & Frenette P.S. (2019). Engineering a haematopoietic stem cell niche by revitalizing mesenchymal stromal cells. Nature cell biology, 21(5), 560-567.
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4

Isolation and Staining of CD4+ T Cells

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Single cell suspensions were made from the spleen after which red blood cells were lysed using NH4Cl buffer. CD4+ T cells were purified by magnetic cell sorting as per manufacturer's instructions (Miltenyi Biotech). CD4+ T cells were re-suspended in PBS and then labelled with the probes for 2 min at the stated concentrations at 37 °C. Cells were washed twice with cell culture medium, after which they were re-suspended in FACS buffer (PBS, 2% FCS, 0.01% sodium azide) or appropriate cell culture medium. For flow cytometric analysis, cells were then incubated with antibodies for 20 min at 4 °C. The antibodies used were anti-CD4-e450 or anti-CD4-PE, anti-CD45.1-FITC or anti-CD45.1-PE, and anti-CD11b-APC (all from eBioscience). Samples were also stained with a fixable viability dye (conjugated with eFluor455, eBioscience) prior to surface staining. Flow cytometry data were collected using an LSR Fortessa (BD Biosciences) and analysed using FlowJo software.
Mellanby R.J., Scott J.I., Mair I., Fernandez A., Saul L., Arlt J., Moral M, & Vendrell M. (2018). Tricarbocyanine N-triazoles: the scaffold-of-choice for long-term near-infrared imaging of immune cells in vivo †Electronic supplementary information (ESI) available: Additional details for theoretical calculations, chemical synthesis and characterisation of all probes; supplementary figures and experimental procedures for all assays performed with murine and human CD4+ T cells. See DOI: 10.1039/c8sc00900g. Chemical Science, 9(36), 7261-7270.
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5

Multicolor Flow Cytometry Panel

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Anti‐B220 Cy5, anti‐B220 Cy3, anti‐CD62L Pacific Orange, anti‐CD4 Alexa488, anti‐IgD Cy5 and anti‐Lyve‐1 Cy3 were provided by E. Kremmer (Helmholtz Zentrum München, Munich, Germany). Anti‐CD4 PerCp, anti‐CD40 APC, anti‐CD80 APC, and anti‐CD45.1 PE were purchased from Bio‐legend. Anti‐CD11b PE‐Cy7, Anti‐CD11c PE‐Cy7, anti‐CD3 PE, anti‐B220 PE, and anti‐CD45.1 FITC were purchased from eBioscience, San Diego, CA. Anti‐CD11b PE was purchased from Caltag Laboratories, Anti‐CD11c PE from BD Biosciences, and anti‐pDCA‐1 APC from Milteyni Biotec.
Kohli K., Janssen A, & Förster R. (2016). Plasmacytoid dendritic cells induce tolerance predominantly by cargoing antigen to lymph nodes. European Journal of Immunology, 46(11), 2659-2668.
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