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Mda mb 436 cells

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MDA-MB-436 cells are a human breast cancer cell line derived from a metastatic site. They are adherent cells that can be used for in vitro studies.

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Lab products found in correlation

Vorinostat, by Selleck Chemicals (2 mentions) Rucaparib, by Selleck Chemicals (2 mentions) Epidermal growth factor (egf), by Cambrex (2 mentions) Bovine pituitary extract, by Cambrex (2 mentions) Mcf10a cells, by American Type Culture Collection (2 mentions) Ovcar8, by American Type Culture Collection (2 mentions) Brca1 d 9 monoclonal and ttk polyclonal antibodies, by Santa Cruz Biotechnology (2 mentions) Auy922, by Selleck Chemicals (2 mentions) Olaparib, by Selleck Chemicals (2 mentions) Mcf 7, by American Type Culture Collection (2 mentions) Mtor inhibitor rapamycin, by Merck Group (2 mentions) Du145, by American Type Culture Collection (2 mentions) Ly294002, by Merck Group (2 mentions) U2os cell, by American Type Culture Collection (2 mentions) Mcf 7 cell, by American Type Culture Collection (2 mentions) 4t1 cell, by American Type Culture Collection (2 mentions) Mda mb 231 cell, by American Type Culture Collection (1 mentions) Du145 cells, by American Type Culture Collection (1 mentions) Sh sy5y cell, by American Type Culture Collection (1 mentions) Bt474 cells, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

Triple-negative MDA-MB-436 human breast adenocarcinoma cancer cells MDA-MB-436

5 protocols using mda mb 436 cells

1

Cell Culture Protocols for Cancer Research

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U2OS cells (American Type Culture Collection, ATCC) were maintained in McCoy’s 5A medium supplemented with 10% fetal bovine serum. MCF-10A cells (ATCC) were cultured in mammary epithelial growth medium containing insulin, hydrocortisone, epidermal growth factor, and bovine pituitary extract (Clonetics). EVSAT cells (Creative Bioarray, NY, USA) were cultured in MEM containing 10% fetal bovine serum. MDA-MB-436 cells (ATCC) were maintained in DMEM medium supplemented with 10% fetal bovine serum. PC3, DU145, ACHN, 786-0, H226, H522, OVCAR-3, OVCAR-8, and MCF7 cells were all obtained from ATCC and maintained according to ATCC instructions. BRCA1 (D-9) monoclonal and TTK polyclonal antibodies were purchased from Santa Cruz (SC-6954, 1:1000) and Cell Signaling (#3255, 1:1000), respectively. ZNF668 antibodies were generated as previously described41 (link). Uncropped scans of the most important western blots are listed as supplementary figures in Supplementary Figure 13. PI3K inhibitor LY-294002 and mTOR inhibitor rapamycin were purchased from Sigma. PARP inhibitors olaparib and rucaparib, HDAC inhibitor vorinostat and Hsp90 inhibitor AUY922 were from Selleckchem. TTK inhibitor AZ3146 was purchased from R&D Systems.
Peng G., Lin C.C., Mo W., Dai H., Park Y.Y., Kim S.M., Peng Y., Mo Q., Siwko S., Hu R., Lee J.S., Hennessy B., Hanash S., Mills G.B, & Lin S.Y. (2014). Genome-wide Transcriptome Profiling of Homologous Recombination DNA Repair. Nature communications, 5, 3361.
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2

Cell Culture Protocols for Cancer Research

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U2OS cells (American Type Culture Collection, ATCC) were maintained in McCoy’s 5A medium supplemented with 10% fetal bovine serum. MCF-10A cells (ATCC) were cultured in mammary epithelial growth medium containing insulin, hydrocortisone, epidermal growth factor, and bovine pituitary extract (Clonetics). EVSAT cells (Creative Bioarray, NY, USA) were cultured in MEM containing 10% fetal bovine serum. MDA-MB-436 cells (ATCC) were maintained in DMEM medium supplemented with 10% fetal bovine serum. PC3, DU145, ACHN, 786-0, H226, H522, OVCAR-3, OVCAR-8, and MCF7 cells were all obtained from ATCC and maintained according to ATCC instructions. BRCA1 (D-9) monoclonal and TTK polyclonal antibodies were purchased from Santa Cruz (SC-6954, 1:1000) and Cell Signaling (#3255, 1:1000), respectively. ZNF668 antibodies were generated as previously described41 (link). Uncropped scans of the most important western blots are listed as supplementary figures in Supplementary Figure 13. PI3K inhibitor LY-294002 and mTOR inhibitor rapamycin were purchased from Sigma. PARP inhibitors olaparib and rucaparib, HDAC inhibitor vorinostat and Hsp90 inhibitor AUY922 were from Selleckchem. TTK inhibitor AZ3146 was purchased from R&D Systems.
Peng G., Lin C.C., Mo W., Dai H., Park Y.Y., Kim S.M., Peng Y., Mo Q., Siwko S., Hu R., Lee J.S., Hennessy B., Hanash S., Mills G.B, & Lin S.Y. (2014). Genome-wide Transcriptome Profiling of Homologous Recombination DNA Repair. Nature communications, 5, 3361.
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Cell Culture Conditions for Diverse Cell Lines

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C6 rat glioma cells (ATCC #CCL-107), HEK293 cells (ATCC #CRL-1573), GT1-7 cells [62 (link)], CLU188 cells [63 (link)], SH-SY5Y cells (ATCC #CRL-2266), SK-N-BE2 cells (ATCC #CRL-2271), iNHA cells [63 (link)], MDA-MB-231 cells (ATCC #HTB-26), MDA-MB-436 cells (ATCC #HTB-130), MDA-MB-453 cells (ATCC #HTB-131), MCF7 cells (ATCC #HTB-22), 4T1 cells (ATCC #CRL-2539), BT474 cells (ATCC #HTB-20), CHO cells (ATCC #CCL-61), OVK18 cells [64 (link)], DU145 cells (ATCC #HTB-81), and HCCLM3 cells [65 (link)] were maintained as a monolayer culture on tissue culture dishes at 37 °C, 5% CO2, 100% humidity in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics. SH-SY5Y cells were differentiated in the presence of 10 μM retinoic acid for 1 week [66 (link)]. OC-k3 cells [67 (link)] were maintained as a monolayer on tissue culture dishes at 33 °C, 10% CO2, 100% humidity in DMEM supplemented with 10% heat-inactivated fetal bovine serum, 50 U/mL of recombinant mouse interferon-γ, and antibiotics. S1P and CYM-5478 were solubilized with bovine serum albumin (0.1% final concentration) prior to treatment.
Wang W., Shanmugam M.K., Xiang P., Yam T.Y., Kumar V., Chew W.S., Chang J.K., Ali M.Z., Reolo M.J., Peh Y.X., Abdul Karim S.N., Tan A.Y., Sanda T., Sethi G, & Herr D.R. (2020). Sphingosine 1-Phosphate Receptor 2 Induces Otoprotective Responses to Cisplatin Treatment. Cancers, 12(1), 211.
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4

Breast Cancer Cell Line Maintenance

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Human breast cancer SUM159PT cells were purchased from Asterand Bioscience (Detroit, MI); MDA-MB-231, MCF-7, and Hs-578-t cells were obtained as cell lines of NCI-60 from the NCI Developmental Therapeutics Program (Bethesda, MD); and MDA-MB-436 cells were obtained from the American Type Culture Collection (Manassas, VA). SUM159PT cells were maintained in Ham's F-12 medium supplemented with 5% fetal bovine serum (FBS), 1 µg/ml hydrocortisone, and 5 µg/ml insulin at 37 °C in 5% CO 2 . MDA-MB-231 cells were cultured in RPMI 1640 medium with 10% FBS, 2 mM glutamine, 50 units/ml penicillin, and 100 µg/ml streptomycin at 37 °C in 5% CO 2 . MCF-7, Hs-578-t, and MDA-MB-436 cells were maintained in DMEM medium supplemented with 10% FBS, 4 mM glutamine, 50 units/ml penicillin, and 100 µg/ml streptomycin at 37 °C in 5% CO 2 . SUM159PT, MDA-MB-231, Hs-578-t, and MDA-MB-436 cells do not express ER, progesterone receptor (PgR), or HER2, while MCF-7 cells express a high level of ER, low level of PgR, and no detectable level of HER2.
Ono H., Sowa Y., Horinaka M., Iizumi Y., Watanabe M., Morita M., Nishimoto E., Taguchi T, & Sakai T. (2018). The histone deacetylase inhibitor OBP-801 and eribulin synergistically inhibit the growth of triple-negative breast cancer cells with the suppression of survivin, Bcl-xL, and the MAPK pathway. Breast cancer research and treatment, 171(1).
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5

Virus-based Breast Cancer Cell Lines

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Construction of MV-m-uPA and MV-h-uPA, virus rescue, propagation and titration were performed as previously reported [24 (link), 36 (link)]. 4T1 cells (murine mammary carcinoma), MDA-MB-231 cells (human breast cancer), MCF-7 cells (human breast cancer), MDA-MB-436 cells (human breast cancer) were purchased from the American Type Culture Collection (ATCC, Manassas, VA). 4T1-luc2 cells stably expressing highly efficient luciferase were from PerkinElmer (Santa Clara CA). Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS), penicillin and streptomycin at 37 °C and 5% CO2. HMEC cells (human mammary epithelial cells) were purchased from Lonza (Walkersville, MD) and maintained in MEGM according to manufacture’s instruction at 37 °C and 5% CO2. NMuMG (murine mammary epithelial cells) were purchased from the ATCC and maintained in DMEM containing 10% fetal bovine serum (FBS), penicillin and streptomycin at 37 °C and 5% CO2. Vero-αHis cells [36 (link)] were grown in DMEM containing 10% FBS at 37 °C and 5% CO2.
Jing Y., Bejarano M.T., Zaias J, & Merchan J.R. (2014). In vivo antimetastatic effects of uPAR retargeted measles virus in syngeneic and xenograft models of mammary cancer. Breast cancer research and treatment, 149(1), 99-108.
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