Anti pirs1

Manufactured by Merck Group

Sourced in United States

Anti-pIRS1 is a laboratory reagent used for the detection and quantification of phosphorylated insulin receptor substrate 1 (pIRS1) in various biological samples. It is a specific antibody that binds to the phosphorylated form of IRS1, a key protein involved in insulin signaling pathways. The core function of Anti-pIRS1 is to provide a tool for researchers to analyze the activation and regulation of insulin signaling in cells and tissues.

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Lab products found in correlation

Anti tubulin, by Merck Group (1 mentions) Anti pkr, by Santa Cruz Biotechnology (1 mentions) Anti irs 1, by Santa Cruz Biotechnology (1 mentions) Anti eif2alpha, by Cell Signaling Technology (1 mentions) Anti p jnk, by Cell Signaling Technology (1 mentions) Anti jnk, by Santa Cruz Biotechnology (1 mentions) Lightcycler 480, by Roche (1 mentions) Rnase free dnase kit, by Qiagen (1 mentions) Superscript 3, by Thermo Fisher Scientific (1 mentions) Anti vinculin, by Merck Group (1 mentions) Anti c ebpβ sc 150, by Santa Cruz Biotechnology (1 mentions) Anti pakt thr308, by Cell Signaling Technology (1 mentions) Anti pparγ, by Cell Signaling Technology (1 mentions) Anti prep1, by Santa Cruz Biotechnology (1 mentions) Anti klf5, by Abcam (1 mentions) Anti phospho c ebpβ thr235, by Cell Signaling Technology (1 mentions) Anti p akt ser473, by Cell Signaling Technology (1 mentions) Anti c ebpα, by Cell Signaling Technology (1 mentions)

2 protocols using anti pirs1

Comprehensive Immunoblotting Antibody Panel

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Anti-PKR (sc-708, Santa Cruz), anti-peIF2alpha (44728G, Invitrogen), anti-eIF2alpha (#9772, Cell Signaling), anti-pJNK (#4668, Cell Signaling), anti-JNK (sc-7345, Santa Cruz), anti-pIRS1 (07–247, Millipore), anti-IRS1 (sc-559, Santa Cruz), anti-pIKKbeta (#2694, Cell Signaling), anti-IKKbeta (#2370, Cell Signaling), and anti-Tubulin (T9026, Sigma).

Taga M., Mouton-Liger F., Sadoune M., Gourmaud S., Norman J., Tible M., Thomasseau S., Paquet C., Nicoll J.A., Boche D, & Hugon J. (2018). PKR modulates abnormal brain signaling in experimental obesity. PLoS ONE, 13(5), e0196983.

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Transcription Factor Expression Analysis

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RNA extraction was processed according to the RNeasy (QIAGEN) protocol. After genomic DNA degradation with the RNase-Free DNase kit (QIAGEN), reverse transcription was performed with the Superscript III (Invitrogen). For Srebf1, Klf4, Klf5, Krox20, C/EBPδ, C/EBPβ, Prep1, Glut4 expression analysis, cDNAs were subjected to qRT-PCR on a Roche LightCycler480 (Roche) using predesigned primers (RealTime ready assays, Roche). Sequences are available upon request. Results were normalized to Gapdh gene expression.
Antibodies used were anti-Prep1 (Santa Cruz sc-25282), anti-C/EBPα (D56F10 Cell signaling Technology #8178), anti-C/EBPβ (sc-150, Santa Cruz Biotechnology, Santa Cruz, USA), anti-phospho-C/EBPβ (Thr235) (Cell Signaling Technology #3084), anti-C/EBPδ (sc-151, Santa Cruz Biotechnology, Santa Cruz, USA), anti-Pparγ (81B8, Cell signaling Technology #2443), anti-pIrs1 (Tyr941) (07-848-I Millipore), anti-pAkt (Ser473) (D9E Cell signaling Technology #4060), anti-pAkt (Thr308) (D25E6 Cell signaling Technology #13038), anti-Klf5 (Abcam ab24331), anti-Vinculin (Sigma V9131).

Maroni G., Tkachuk V.A., Egorov A., Morelli M.J., Luongo R., Levantini E., Blasi F., Magli M.C, & Penkov D. (2017). Prep1 prevents premature adipogenesis of mesenchymal progenitors. Scientific Reports, 7, 15573.

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