Murine splenic NK cells were sorted using BD FACSAriaTMIII (more than 95% purity). Sorted cells were stimulated with IL-15 (100 ng/ml) for 20 min at 37 °C. The stimulation was attenuated by fixation with BD Cytofix™ - Fixation Buffer (BD Pharmingen) for 10 min at 37 °C. Cells were then permeabilized by dropwise adding Perm Buffer III (BD Pharmingen) and incubated for 30 min on ice. The staining with phosphoflow antibodies was done at room temperature for up to one hour. Antibodies (BD Pharmingen) used to detect phosphorylated signaling molecules are anti-p-STAT4 (pY693), anti-p-STAT5 (pY694), anti-p-p38 (pT180/pY182), anti-p-ERK (pT202/pY204), anti-p-AKT (pT308), anti-p-AKT (pS473), anti-p-mTOR (pS2448), anti-4-EBP1 FITC (pT69), and anti-pS6 PE (pS244).
Anti p stat4
Manufactured by BD
Anti-p-STAT4 is a laboratory equipment product designed for the detection and analysis of phosphorylated STAT4 (Signal Transducer and Activator of Transcription 4) in cellular samples. It is a tool used in research and scientific investigations.
Automatically generated - may contain errors
Lab products found in correlation
Anti pstat5, by BD (2 mentions)
Facsariatm 3, by BD (1 mentions)
Cytofix fixation buffer, by BD (1 mentions)
Perm buffer 3, by BD (1 mentions)
Bcl 6, by BD (1 mentions)
Recombinant human il 12, by R&D Systems (1 mentions)
Il 23, by Thermo Fisher Scientific (1 mentions)
Anti pstat3, by BD (1 mentions)
Percp cy5.5 anti ifn γ, by Thermo Fisher Scientific (1 mentions)
Anti cd4 apc cy7, by BD (1 mentions)
Anti t bet, by Thermo Fisher Scientific (1 mentions)
Pacificblue anti cd20, by BioLegend (1 mentions)
Efluor660 anti il 21, by Thermo Fisher Scientific (1 mentions)
Biotin pd 1, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 anti cxcr5, by BD (1 mentions)
Anti t bet, by BD (1 mentions)
Alexa fluor 488 anti il 10, by Thermo Fisher Scientific (1 mentions)
Sa bv605, by BioLegend (1 mentions)
Anti cd27, by BD (1 mentions)
Anti pstat6, by BD (1 mentions)
2 protocols using anti p stat4
1
Phosphoflow Analysis of Murine Splenic NK Cells
2
Multiparametric Profiling of T Cell Subsets
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eFluor660-anti–IL-21, Alexa Fluor 488–anti–IL-10, PerCP-Cy5.5-anti–IFN-γ, FITC-anti-CD45RA, PE-anti-ICOS, and anti-Tbet, and biotin–PD-1 were from eBioscience. Alexa Fluor 488–anti-GATA3, Alexa Fluor 647–anti-CXCR5, anti-pSTAT4, anti-pSTAT5, anti-pSTAT6, APC-anti-CD10, APC-Cy7-anti-CD4, BV605-anti-IgG, BV421-CD40L, BV711-anti–IL-2, PE-anti-pSTAT1, anti-RORγt, and Bcl-6, PE-Cy7-anti-CD25, and anti-CD27, PerCP-Cy5.5-anti-CD127, anti-pSTAT3, and anti-Tbet, SA-PerCpCy5.5, and IFN-γ were obtained from BD. Pacific Blue–anti-CD20 and SA-BV605 were purchased from BioLegend. Recombinant human IL-12 was purchased from R&D Systems. TGF-β, IL-1β, IL-6, IL-21, and IL-23 were obtained from PeproTech. PGE2 was purchased from Sigma-Aldrich. Human IL-4 and IL-10 were provided by R. de Waal Malefyt (DNAX Research Institute, Palo Alto, CA).
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