The immunohistochemical detection of indicated antibodies was performed at Molecular Cytology Core Facility of Memorial Sloan Kettering Cancer Center using Discovery XT processor (Ventana Medical Systems). The tissue sections were deparaffinized with EZPrep buffer (Ventana Medical Systems), antigen retrieval was performed with CC1 buffer (Ventana Medical Systems) and sections were blocked for 30 minutes with Background Buster solution (Innovex)(for rabbit, mouse and chicken Abs, for rat and goat Abs it should be 10% normal rabbit serum in PBS+2%BSA). Antibodies were applied and sections were incubated for 5 hours, followed by 60 minutes incubation with biotinylated goat anti-rabbit IgG (Vector labs, cat#PK6101) (biotinylated horse anti-mouse IgG (Vector Labs, cat# MKB-22258); biotinylated goat anti-chicken (Vector Labs, cat#BA-9010); biotinylated rabbit anti-goat IgG (Vector, cat #BA-5000)) at 1:200 dilution. The detection was performed with DAB detection kit (Ventana Medical Systems) according to manufacturer instruction. Slides were counterstained with hematoxylin and coverslipped with Permount (Fisher Scientific). Staining quantification was performed using MetaMorph Software.
Biotinylated goat anti chicken
Manufactured by Vector Laboratories
Sourced in United Kingdom
Biotinylated goat anti-chicken is a secondary antibody produced in goats and labeled with biotin. It is designed for use in various immunoassay and molecular biology techniques that require the detection of chicken-derived targets.
Automatically generated - may contain errors
Lab products found in correlation
Biotinylated horse anti mouse igg, by Vector Laboratories (1 mentions)
Permount, by Thermo Fisher Scientific (1 mentions)
Dab detection kit, by Roche (1 mentions)
Cc1 buffer, by Roche (1 mentions)
Biotinylated goat anti rabbit igg, by Vector Laboratories (1 mentions)
Discovery xt processor, by Roche (1 mentions)
Ezprep buffer, by Roche (1 mentions)
Streptavidin alexa 488, by Thermo Fisher Scientific (1 mentions)
Rabbit polyclonal anti iba1, by Fujifilm (1 mentions)
Goat anti chicken alexa 488, by Thermo Fisher Scientific (1 mentions)
Chicken polyclonal anti gfp, by Abcam (1 mentions)
Cy3 conjugated goat anti mouse, by Jackson ImmunoResearch (1 mentions)
Cy5 conjugated goat anti rabbit, by Jackson ImmunoResearch (1 mentions)
Cy3 conjugated donkey anti rabbit, by Jackson ImmunoResearch (1 mentions)
Vectastain abc kit, by Vector Laboratories (1 mentions)
Avidin biotin blocking kit, by Vector Laboratories (1 mentions)
Antigen unmasking solution, by Vector Laboratories (1 mentions)
Anti claudin 1, by Abcam (1 mentions)
3 protocols using biotinylated goat anti chicken
1
Immunohistochemical Staining Protocol
2
Confocal Microscopy of Retrovirally Labeled Neurons
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For confocal microscopy, rats were perfused with ice-cold saline and 4% paraformaldehyde 3 or 6 weeks after retroviral injections. Brains were processed for immunohistochemistry as previously described [11 (link)]. The following primary and secondary antibodies were used: rabbit polyclonal anti-Iba1 (1:1000, Wako, Japan), chicken polyclonal anti-GFP (1:5000, Abcam, UK), mouse monoclonal anti-gephyrin (1:500, Synaptic Systems, Germany), Cy3-conjugated donkey anti-rabbit (1:200, Jackson Immunoresearch, UK), Cy3-conjugated goat anti-mouse (1:200, Jackson Immunoresearch), biotinylated goat anti-chicken (Vector laboratories), Cy5-conjugated goat anti-rabbit (1:200, Jackson Immunoresearch), Alexa-488 goat anti-chicken (1:200, Invitrogen, Sweden), and Alexa-488 streptavidin (1:200, Invitrogen).
3
Immunohistochemical Detection of Claudin-1
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Paraffin sections were blocked with avidin/biotin blocking kit (Vector Laboratories, Burlingame, CA) and 3% H 2 O 2 . The sections were subjected to microwave antigen retrieval in antigen unmasking solution (Vector Laboratories) followed by incubation with the primary (anti-claudin-1, Abcam) and secondary antibodies. As secondary antibody, we used biotinylated goat anti-chicken (Vector Laboratories). Detection was carried out with vectastain ABC kit (Vector Laboratories) with the use of peroxidase as label and 3,3-diaminobenzidine as substrate and nickel chloride enhancement.
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