Anhydrous sodium acetate

Manufactured by Thermo Fisher Scientific

Sourced in United States, China

Anhydrous sodium acetate is a chemical compound with the formula CH3COONa. It is a crystalline solid that is highly soluble in water and is commonly used as a buffer in laboratory settings to maintain specific pH levels in solutions.

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Lab products found in correlation

Methanol, by Thermo Fisher Scientific (4 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Formic acid, by Thermo Fisher Scientific (2 mentions) Equol, by Merck Group (2 mentions) Lyophilized glucuronidase sulfatase from helix pomatia, by Merck Group (2 mentions) Hplc grade water, by Thermo Fisher Scientific (2 mentions) Hydrochloric acid (hcl), by Thermo Fisher Scientific (2 mentions) Potassium chloride (kcl), by Thermo Fisher Scientific (2 mentions) Diethyl ether et2o, by Thermo Fisher Scientific (1 mentions) Disodium hydrogenphosphate dodecahydrate, by Thermo Fisher Scientific (1 mentions) Sodium pyruvate, by Merck Group (1 mentions) Hplc grade solvents water and acetonitrile, by Thermo Fisher Scientific (1 mentions) Silver nitrate, by Merck Group (1 mentions) Trifluoroacetic acid, by Thermo Fisher Scientific (1 mentions) Sodium formate, by Merck Group (1 mentions) Celite, by Merck Group (1 mentions) Total ros superoxide detection kit, by Enzo Life Sciences (1 mentions) Benzo h quinoline, by Merck Group (1 mentions) Meod d4, by Merck Group (1 mentions) β nicotinamide adenine dinucleotide reduced disodium salt nadh, by Merck Group (1 mentions)

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Sodium acetate (111 citations)

11 protocols using anhydrous sodium acetate

Isoflavone and Metabolite Quantification

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Anhydrous sodium acetate, and diethyl ether (Et₂O) as well as HPLC or MS-grade acetonitrile, formic acid (≥98% purity), methanol, and water were purchased from Fisher Scientific (Fairlawn, NJ). Lyophilized glucuronidase/sulfatase from Helix pomatia, dimethyl sulfoxide, and equol were obtained from Sigma-Aldrich (St. Louis, MO). Daidzein, genistein, glycitein, daidzin, genistin, glycitin, acetyl daidzin, malonyl genistin, acetyl genistin were from LC laboratories division (PKC Pharmaceuticals, Inc, Woburn, MA) and malonyl daidzin, and malonyl glycitin from Wako Chemicals USA, Inc (Richmond, VA). Isoflavone metabolites (dihydrodaidzein, dihydrogenistein, ODMA, and 6-OH-ODMA) were from Plantech (Reading, UK).

Ahn-Jarvis J.H., Clinton S.K., Grainger E.M., Riedl K.M., Schwartz S.J., Lee M.L., Cruz-Cano R., Young G.S., Lesinski G.B, & Vodovotz Y. (2015). Isoflavone pharmacokinetics and metabolism after consumption of a standardized soy and soy-almond bread in men with asymptomatic prostate cancer. Cancer prevention research (Philadelphia, Pa.), 8(11), 1045-1054.

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Rhodium-Catalyzed Oxidation Reactions

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Rhodium(III) trichloride hydrate was purchased from Precious Metals Online (PMO Pty Ltd.) and used as received. [Cp*Rh(μ-Cl)Cl]₂, benzo[h]quinoline, silver nitrate, sodium formate, sodium pyruvate, β-nicotinamide adenine dinucleotide reduced disodium salt (NADH), and celite were purchased from Sigma-Aldrich. MeOD-d₄, D₂O, and CDCl₃-d₁ for NMR spectroscopy and Quantofix® peroxides test sticks (1-100 mg/L range) were purchased from Sigma-Aldrich. Reduced glutathione was obtained from Alfa Aesar. Disodium hydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate, anhydrous sodium acetate, HPLC grade solvents (water and acetonitrile) with added trifluoroacetic acid with analytic grade, anhydrous DCM/pyridine and laboratory grade solvents used in syntheses were purchased from fisher scientific. A549 human lung and A2780 human ovarian cancer cell lines were purchased from the European Collection of Animal Cell Culture (ECACC, Salisbury, U.K.). Roswell Park Memorial Institute (RPMI-1640) medium, and phosphate-buffered saline (PBS) were purchased from PAA Laboratories GmbH. The total ROS/superoxide detection kit was purchased from Enzo Life Sciences. The apoptosis detection regents were purchased from Abcam.

Zhang W.Y., Bridgewater H.E., Banerjee S., Soldevila-Barreda J.J., Clarkson G.J., Shi H., Imberti C, & Sadler P.J. (2019). Ligand-Controlled Reactivity and Cytotoxicity of Cyclometalated Rhodium(III) Complexes. European journal of inorganic chemistry, 2020(11-12), 1052-1060.

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Comprehensive Metabolite Profiling with HPLC-MS

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All reagents and solvents were used as received without further processing. Acetone, concentrated hydrochloric acid, phloroglucinol, sodium sulfite, glycerol, ammonium iron (III) sulfate dodecahydrate and (+)-catechin hydrate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol, dichloromethane, absolute ethanol, 1-butanol, ascorbic acid and anhydrous sodium acetate were purchased from Fisher Scientific (Hampton, VA, USA). Choline chloride, betaine, proline and (D)-glucose were purchased from Acros Organics (Waltham, MA, USA). Urea and DL-malic acid were purchased from VWR (Radnor, PA, USA). Toyopearl HW-40S resin was acquired from Tosoh (Tokyo, Japan). HPLC-grade water, acetonitrile and formic acid were acquired from Fisher Scientific.
The High-Performance Liquid Chromatography (HPLC) system was an Accela from Fisher Scientific with an Accela 600 LC pump and an Accela 80Hz Diode-Array detector (DAD). Chromatographic separation was carried out with a Hypersil GOLD C18 (2.1 × 100 mm with 1.9 µm particle size) column from Thermo Scientific. The MS system used for compound identification was a LCQ Fleet ion trap mass spectrometer from Thermo Finnigan with an electrospray ionization (ESI) source. Data acquisition was performed by using the Xcalibur data system from Thermo Finnigan (Waltham, MA, USA).

Neto R.T., Santos S.A., Oliveira J, & Silvestre A.J. (2020). Tuning of Proanthocyanidin Extract’s Composition through Quaternary Eutectic Solvents Extraction. Antioxidants, 9(11), 1124.

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Isoflavone Quantification Protocol

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From Fisher Scientific (Fairlawn, OH, USA), anhydrous sodium acetate and solvents (diethyl ether, formic acid, acetonitrile, methanol, and water) which were HPLC-grade or higher were purchased. Lyophilized glucuronidase/sulfatase from Helix pomatia, dimethyl sulfoxide, and equol were obtained from Sigma-Aldrich (St. Louis, MO, USA). Daidzein, genistein, glycitein, daidzin, genistin, glycitin, acetyl daidzin, malonyl genistin, and acetyl genistin were obtained from LC laboratories division (PKC Pharmaceuticals, Inc., Woburn, MA, USA), and malonyl daidzin and malonyl glycitin from Wako Chemicals USA, Inc., (Richmond, VA, USA). Isoflavone metabolites (dihydroDaidzein DHD, dihydrogenistein DHG, ODMA, and 6-OH-ODMA) were acquired from Plantech (Reading, UK). The following authenticated isoflavone standards were purchased from Sigma-Aldrich (St. Louis, MO, USA): Daidzein, genistein, glycitein, and equol. Food isoflavone standards (daidzin, genistin, glycitin, acetyl daidzin, malonyl genistin, and acetyl genistin) were obtained from LC Laboratories division (PKC Pharmaceuticals, Inc., Woburn, MA, USA).

Ahn-Jarvis J.H., Sosh D., Lombardo E., Lesinski G.B., Conwell D.L., Hart P.A, & Vodovotz Y. (2023). Short-Term Soy Bread Intervention Leads to a Dose-Response Increase in Urinary Isoflavone Metabolites and Satiety in Chronic Pancreatitis. Foods, 12(9), 1762.

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Synthesis and Characterization of Flavonoid-Functionalized Branched PEI

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Branched polyethyleneimine (PEI) (M_w = 600, 1800, 10 000 and 70 000), 3-carboxybenzoboroxole (CBO), rutin (Ru), quercetin (Qu), luteolin (Lu), myricetin (Myr), genistein (Gen), isorhamnetin (Iso), and kaempferol (Kae) were from J&K Scientific (Shanghai, China). Ferric chloride hexahydrate (FeCl₃·6H₂O), anhydrous sodium acetate (NaOAc), ethylene glycol, sodium cyanoborohydride, anhydrous methanol, glutaraldehyde, 1,6-hexanediamine, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) were purchased from Alfa Aesar (Tianjin, China). All used reagents were of analytical grade. All commercially available reagents were used without further purification. Water used in all the experiments was purified by a Milli-Q Advantage A10 ultrapure purification system (Millipore, Milford, USA).

Li D., Liu Z., Song R., Yang W., Zhai S, & Wang W. (2019). Branched polyethyleneimine-assisted 3-carboxybenzoboroxole improved Wulff-type boronic acid functionalized magnetic nanoparticles for the specific capture of cis-diol-containing flavonoids under neutral conditions. RSC Advances, 9(65), 38038-38046.

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Synthesis and Characterization of Isotopically Labeled Standards

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1-NA, 2-NA, 3-ABP, 4-ABP, 1-NA-d₇, 2-NA-d₇, 3-ABP-d₉ and 4-ABP-d₉ were analytical standard and obtained from Dr. Ehrenstorfer (purity≥98%, Germany). Acetonitrile and methanol were HPLC grade and purchased from Dikama Corp. (USA). Formic acid was HPLC grade and obtained from TEDIA Company Inc. (USA). Hydrochloric acid and sodium hydroxide were of analytical reagent and obtained from ACROS ORGANICS (USA). Aqueous ammonia was of analytical reagent (CNW Technologies, Germany). All other reagents were analytical standard. Water was purified with a Milli-Q system (Millipore, Bedford, MA, USA).
FeCl₃·6H₂O (97%) was ACS reagent from Sigma-Aldrich (USA), ethylene glycol (99.9%) was purchased from KEMIOU Chemical Reagents Ltd. (Tianjin, China). Anhydrous sodium acetate with purity of 99% was obtained from Alfa Aesar (USA). 1,6-Diaminohexane (98%) was obtained from Sigma-Aldrich (USA). 2,4,6-Trihydroxybenzene-1,3,5-tricarbaldehyde (Tp, 95%) was obtained from Ark Pharm, Inc. (United States). p-Phenylenediamine (Pa-1, 99%) was purchased from J&K Scientific Ltd. (China). Other reagents were analytical reagent, such as dimethylformamide and 95% ethyl alcohol.

Yu J., Wang B., Cai J., Yan Q., Wang S., Zhao G., Zhao J., Pan L, & Liu S. (2020). Selective extraction and determination of aromatic amine metabolites in urine samples by using magnetic covalent framework nanocomposites and HPLC-MS/MS. RSC Advances, 10(47), 28437-28446.

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Neurochemical Analysis of Pharmacological Agents

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Commercially available drugs used in this study were purchased: pentobarbital (Sigma-Aldrich), Estazolam tablet (1.0 mg/60 mg; Shandong Xinyi Pharmaceutical Co., Ltd., Shandong, China), anhydrous sodium acetate (Thermo Fisher Scientific), citric acid (Coolaber), sodium octane sulfonate (Adamas-beta), EDTA-2Na (Beijing Biodee Biotechnology Co., Ltd., Beijing, China), KCl (Thermo Fisher Scientific), perchlorate (Tianjin Damao Chemical Reagent Factory, Tianjin, China). HPLC-grade water and methanol were obtained from Fisher Scientific (Thermo Fisher Scientific). 3,4-Dihydroxybenzylamine hydrobromide (DHBA), norepinephrine (NE), 3,4-dihydroxy-phenyl acetic acid (DOPAC), dopamine (DA), 5-hydroxyindoleacetic acid (5-HIAA), homovanillic acid (HVA), and serotonin (5-HT) were purchased from Sigma-Aldrich. PCPA, caffeine, and 5-HTP were obtained from Melonepharma Co., Ltd. (Dalian, China).

Shi R., Han Y., Yan Y., Qiao H.Y., He J., Lian W.W., Xia C.Y., Li T.L., Zhang W.K, & Xu J.K. (2019). Loganin Exerts Sedative and Hypnotic Effects via Modulation of the Serotonergic System and GABAergic Neurons. Frontiers in Pharmacology, 10, 409.

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Analytical Grade Chemicals for Extraction and Characterization

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Several chemicals
of analytical grade that were used for extraction and characterization
were purchased from Sigma-Aldrich (Castle Hill, NSW, Australia) including
2,2′-azino-bis (3 ethylbenzothiazoline-6-sulfonic acid) (ABTS),
Folin and Ciocalteu’s phenol reagent, 2,2′-diphenyl-1-picrylhydrazyl
(DPPH), l-ascorbic acid, vanillin, hexahydrate aluminum chloride,
ferric chloride, gallic acid, quercetin, and 2,4,6-tripyridyl-s-triazine (TPTZ). The supplier of hydrochloric acid, glacial
acetic acid, hydrated sodium acetate, methanol, and anhydrous sodium
acetate was Thermo Fisher Scientific Inc. (Waltham, MA, USA). Sodium
carbonate (anhydrous) was supplied by Chem-Supply Pty Ltd. (Adelaide,
SA, Australia), while sulfuric acid (98%) was purchased from RCI Labscan
(Rongmuang, Thailand). Deionized water (resistivity, 18.2 MΩ/cm)
was prepared by a Millipore Milli-Q Gradient Water Purification System
(Darmstadt, Germany) and the filtration was processed by a 0.22 μm
type Millipak Express 20 filter (Milli-Q, Darmstadt, Germany).

Zou X., BK A., Rauf A., Saeed M., Al-Awthan Y.S., A. Al-Duais M., Bahattab O., Hamayoon Khan M, & Suleria H.A. (2021). Screening of Polyphenols in Tobacco (Nicotiana tabacum) and Determination of Their Antioxidant Activity in Different Tobacco Varieties. ACS Omega, 6(39), 25361-25371.

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Alginate Bead Synthesis Protocol

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Formic, acetic, and hydrochloric acids, reagent ethanol, calcium carbonate, potassium chloride, sodium acetate anhydrous, Tween^® 80, and sodium citrate were purchased from Fisher Scientific (Ottawa, ON, Canada). Calcium chloride 96% anhydrous and calcium carbonate 98% pure were purchased from Acros Organics (Fairlawn, NJ, USA). Low‐viscosity sodium alginate Protanal LFR5/60 (MW = 40 kDa, 65–75% guluronic acid) was kindly donated by FMC BioPolymer (Philadelphia, PA, USA).

Celli G.B., Ghanem A, & Brooks M.S. (2016). Development and evaluation of floating alginate microspheres for oral delivery of anthocyanins – A preliminary investigation. Food Science & Nutrition, 5(3), 713-721.

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Detailed Reagents and Protocols

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Ethanol (96%) was obtained from J.T. Baker (Center Valley, PA, United States). Acetic acid (96%), potassium chloride, and hydrochloric acid (36%) were from Merck (Kenilworth, NJ, United States) and Fisher Chemical (Thermo Fisher Scientific, Waltham, MA, United States), respectively. Phosphoric acid (≥99%), Folin-Ciocalteu’s phenol reagent, sodium carbonate, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), sodium fluoride, ascorbic acid (99%) and Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Sodium acetate (anhydrous) was purchased from Fisher Chemical (Thermo Fisher Scientific, Waltham, MA, United States). Potassium persulfate (K₂S₂O₈) and gallic acid (≥99%) were purchased from Carl Roth (Karlsruhe, Germany). Methanol (HPLC-grade) was purchased from VWR Chemicals (Milan, Italy), and meta-Phosphoric acid (≥99%) and monopotassium phosphate ( ≥ 99%) were from Thermo Fisher Scientific Inc. (Waltham, MA, United States). The ultrapure water was prepared with a Milli-Q-water purification system (EMD Millipore Corporation, Billerica, MA, United States).

Soppelsa S., Kelderer M., Casera C., Bassi M., Robatscher P, & Andreotti C. (2018). Use of Biostimulants for Organic Apple Production: Effects on Tree Growth, Yield, and Fruit Quality at Harvest and During Storage. Frontiers in Plant Science, 9, 1342.

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