Protein (30–40μg) was separated on a 4–20% gradient acrylamide gel (Bio-Rad, Hercules, CA). The proteins transferred onto nitrocellulose membranes were detected using antibodies against the proteins of interest (Dnmt1 and Tet2 from Abcam, and β-actin Cat # A-5316 from Sigma-Aldrich, St. Louis, MO). Images were quantified by Carestream MI software (Carestream, Rochester, NY), and the intensities of the target bands were normalized to those of the loading control, β-actin.
β actin cat a 5316
Manufactured by Merck Group
β-actin Cat # A-5316 is a protein that is commonly used as a reference standard in various biological applications, such as Western blotting and immunohistochemistry. It is a component of the cytoskeleton and is involved in cell structure and motility.
Automatically generated - may contain errors
2 protocols using β actin cat a 5316
1
Western Blot Quantification Protocol
2
Western Blot Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein (30–40μg) was separated on a 4–20% gradient acrylamide gel (Bio-Rad, Hercules, CA). The proteins transferred onto nitrocellulose membranes were detected using antibodies against the proteins of interest (Dnmt1 and Tet2 from Abcam, and β-actin Cat # A-5316 from Sigma-Aldrich, St. Louis, MO). Images were quantified by Carestream MI software (Carestream, Rochester, NY), and the intensities of the target bands were normalized to those of the loading control, β-actin.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!