Psa klk3

Manufactured by Cell Signaling Technology

Sourced in United States

PSA/KLK3 is a lab equipment product offered by Cell Signaling Technology. It is a protein that functions as a serine protease involved in the liquefaction of seminal coagulum.

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Lab products found in correlation

4 protocols using psa klk3

Probing Kinase Inhibitors and Signaling

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ENZ and GSK1120212, PD0325901, GDC-0994 were purchased from Selleckchem (Houston, TX) and were dissolved in DMSO to prepare 50 mM, 10 mM, 50 mM, and 1 mM stock solutions, respectively. AS602801 was purchased from Cayman Chemical (Ann Arbor, MI) and was dissolved in DMSO to prepare a 25 mM stock solution. AS602801 has previously been shown to be a highly specific JNK inhibitor [15 ]. SP600125 also inhibits JNK as a reversible ATP-competitive inhibitor with more than 20-fold selectivity over other kinases including Erk, p38 MAPKs, MKKs, and PKCs [16 (link),17 (link)] and was a kind gift of Dr. Haian Fu (Emory University). All stock solutions were stored at −20°C for in vitro studies. For in vivo experiments, the ENZ stock solution was diluted in 20% Kolliphor RH40 (Sigma-Aldrich) to prepare 200 μl solutions for each injection. Antibodies were purchased from Cell Signaling Technology, Inc.: Phospho-SAPK/JNK (Cat #9251), SAPK/JNK (Cat #9252), Phospho-C-Jun (Cat #9261), C-Jun (Cat #9165), Phospho-Erk1/2 (Cat #9101), Erk1/2 (Cat #9102), PSA/KLK3 (Cat #5365), AR (Cat #3202), and GAPDH (Cat #2118).

Li Z., Sun C., Tao S., Osunkoya A.O., Arnold R.S., Petros J.A., Zu X, & Moreno C.S. (2020). The JNK inhibitor AS602801 Synergizes with Enzalutamide to Kill Prostate Cancer Cells In Vitro and In Vivo and Inhibit Androgen Receptor Expression. Translational Oncology, 13(4), 100751.

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Comprehensive Protein Expression Analysis

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AR(rabbit monoclonal, 1:1000, #5153), PSA/KLK3(rabbit monoclonal, 1:1000, #5365), TOMM20(rabbit monoclonal, 1:1000, #42,406), ATG5(rabbit monoclonal, 1:1000, #12994S), LC3I/II(rabbit monoclonal, 1:1000, #12,741), Akt (rabbit monoclonal, 1:1000, #4691), Phospho-Akt (Ser473)(rabbit monoclonal, 1:1000, #4060), Phospho-Akt (Thr308)(rabbit monoclonal, 1:1000, #13,038), Nanog(rabbit monoclonal, 1:1000, #4903), Sox2(rabbit monoclonal, 1:1000, #3579), ALDH1A1(rabbit monoclonal, 1:1000, #36671S) and VDAC(rabbit monoclonal, 1:1000, #4661) antibodies were purchased from Cell Signaling Technology (CST). NCAM1(rabbit polyclonal antibody, 1:1000, A7913), β-Actin(mouse polyclonal, 1:5000, AC004) and α-Tublin(mouse polyclonal, 1:5000, AC012) antibodies were purchased from ABclonal Technology (Upper Heyford, UK). NSE (rabbit monoclonal, 1:1000, ab180943) and Syp (Rabbit monoclonal, 1:1000, ab184176) were purchased from Abcam (Shanghai, China). ALDH1A1(mouse monoclonal antibody, 1:500,sc-166362), TOMM70(mouse monoclonal antibody, 1:500, sc-390545),AR(rabbit polyclonal, 1:1000, Sc-815X) antibodies were purchased from Santa cruz. E-cadherin (rabbit polyclonal, 1:1000, GTX100443),BRN2(rabbit polyclonal, 1:1000, GTX114650) and N-cadherin (rabbit polyclonal, 1:1000, GTX127345) were purchased from Genetex.

Yin L., Ye Y., Zou L., Lin J., Dai Y., Fu Y., Liu Y., Peng Y., Gao Y., Fu Y., Qi X., Deng T., Zhang S, & Li X. (2023). AR antagonists develop drug resistance through TOMM20 autophagic degradation-promoted transformation to neuroendocrine prostate cancer. Journal of Experimental & Clinical Cancer Research : CR, 42, 204.

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Protein Analysis by Western Blotting

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Cells were harvested and lysed by resuspension in protein sample buffer. DNA from samples was fragmented by sonication. Total protein from each sample was separated using the Laemmli method [26 (link)]. After blocking, the membranes were immunoblotted overnight at 4 °C with primary antibodies to LSD1, PSA/KLK3, androgen receptor and cleaved PARP (Cell Signalling) and β-Actin (Sigma-Aldrich) as previously described [27 (link)].

Regufe da Mota S., Bailey S., Strivens R.A., Hayden A.L., Douglas L.R., Duriez P.J., Borrello M.T., Benelkebir H., Ganesan A., Packham G, & Crabb S.J. (2018). LSD1 inhibition attenuates androgen receptor V7 splice variant activation in castration resistant prostate cancer models. Cancer Cell International, 18, 71.

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Antibody-based Protein Detection

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Antibodies were used according to manufacturer instructions. PARP [#9542, Santa Cruz (Dallas, Texas, USA)], PSA/KLK3 [D6B1, Cell Signalling (Danvers, Massachusetts, USA)], Rad51 (sc-398587, Santa Cruz), DNA-PK [ab70250, Abcam (Cambridge,UK)] and β-Actin (C4: sc-47778) primary antibodies were used in conjunction with HRP conjugated mouse and rabbit secondary antibodies (Life Technologies, USA).

Wright T.C., Dunne V.L., Alshehri A.H., Redmond K.M., Cole A.J, & Prise K.M. (2021). Abiraterone In Vitro Is Superior to Enzalutamide in Response to Ionizing Radiation. Frontiers in Oncology, 11, 700543.

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