Plan apo vc 60 h objective

MDA-MB-468 cells were plated onto glass coverslips and allowed to adhere overnight. After treatment with maximiscin for 18 h, cells were fixed with paraformaldehyde and subsequently incubated with a blocking solution of 10% bovine calf serum in DPBS for 20 min at room temperature. Cells were then incubated with a primary antibody against γ-H2A.X (Cell Signaling Technology) diluted in 1% bovine serum albumin/0.3% Triton X-100 in DPBS. After incubation, cells were washed with DPBS and incubated with an Alexafluor 594-conjugated secondary antibody (Life Technologies). Coverslips were washed with PBS and stained with NucBlue live cell stain (Life Technologies) diluted in DPBS. Coverslips were mounted and visualized with an Eclipse 80i fluorescence microscope using a Plan Apo VC 60× H objective (Nikon, Tokyo, Japan). Images were captured with a CoolSNAP HQ2 camera (Photometrics, Tuscon, AZ, USA) and NIS Elements software (Nikon).

PC-3 and HeLa cells were plated onto glass coverslips and allowed to
adhere overnight before compound addition. After treatment with the compounds
for 18 h, cells were fixed with methanol (4 °C) for 5 min and
subsequently incubated with a blocking solution of 10% bovine calf serum
in DPBS for 20 min at room temperature. Cells were then incubated with a
monoclonal β-tubulin antibody (1:400; Sigma T4026) for 2 h at 37
°C. After incubation, cells were washed three times with 1%
bovine serum albumin (BSA) in DPBS and then incubated with a FITC-conjugated
sheep anti-mouse IgG (1:200; Sigma F3008) for 1 h at 37°C. Coverslips
were then washed three times with BSA in PBS and stained with 0.1 µg/mL
DAPI (Sigma D9564) in DPBS for 10 min at room temperature. Coverslips were
mounted on slides and visualized with an Eclipse 80i fluorescence microscope
with a Plan Apo VC 60× H objective (Nikon). Images were captured with a
CoolSNAP HQ2 camera (Photometrics) using NIS Elements software (Nikon).