Tram antibody

Manufactured by Santa Cruz Biotechnology

The TRAM antibody is a specific antibody used for the detection and study of the TRAM (Toll-interacting adapter molecule) protein. TRAM is a key component of the Toll-like receptor signaling pathway, which plays a crucial role in the innate immune response. The TRAM antibody can be used in various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to investigate the expression, localization, and function of the TRAM protein.

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Lab products found in correlation

Gapdh 2118, by Cell Signaling Technology (2 mentions) Fadd antibody 05 486, by Merck Group (2 mentions) Zbp1 antibody ag 20b 0010 c100, by Adipogen (1 mentions) Anti mouse igg h l dylight 800 4x peg conjugate, by Cell Signaling Technology (1 mentions) Dylight 800 4x peg conjugate, by Cell Signaling Technology (1 mentions)

2 protocols using tram antibody

Western Blot Analysis of Signaling Proteins

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After indicated treatments, cells were lysed in 1X Laemmli Buffer containing 5% β-mercaptoethanol, boiled for 15 min, and incubated on ice for 15 min. Primary antibodies against p38 (9212), p-p38 (4511), ERK1/2 (4696), p-ERK1/2 (4370), JNK (9255), p-JNK (9255), pIRF3 (29047), IκBα (4814), p-IκBα (9246), full-length CASP8 (4790), cleaved CASP8 (8592), CASP3 (9665), CASP7 (9492), RIPK1 (3493), pRIPK1 (Ser166) (31122), pRIPK1 (Ser321) (83613), TRAF3 (4729), TBK1 (3504), NEMO (2685), A20 (5630), p65 (8242), p-p65 (3033), and GAPDH (2118) were purchased from Cell Signaling Technologies. ZBP1 antibody (AG-20B-0010-C100) was purchased from Adipogen. GSDMD (ab209845) was purchased from Abcam. FADD antibody (05-486) was purchased from Millipore Sigma. TRAM antibody was purchased from Santa Cruz (sc-376076). M1-ubiquitin antibody (MABS451) was purchased from Sigma. Secondary antibodies, anti-rabbit IgG (H+L) (DyLight 800 4X PEG Conjugate) (5151), and anti-mouse IgG (H+L) (DyLight 800 4X PEG Conjugate) (5257) were purchased from Cell Signaling Technologies.

Muendlein H.I., Connolly W.M., Magri Z., Jetton D., Smirnova I., Degterev A., Balachandran S, & Poltorak A. (2022). ZBP1 promotes inflammatory responses downstream of TLR3/TLR4 via timely delivery of RIPK1 to TRIF. Proceedings of the National Academy of Sciences of the United States of America, 119(24), e2113872119.

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Western Blot Analysis of Apoptosis Signaling

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After indicated treatments, cells were lysed in 1X Laemmli Buffer containing 5% β-mercaptoethanol, boiled for 15 min and incubated on ice for 15 min. Primary antibodies against p38 (9212), p-p38 (4511), ERK1/2 (4696), p-ERK1/2 (4370), IκBα (4814), RIPK1 (3493), TRAF3 (4729), TBK1 (3504), NEMO (2685), CASP8 (8592), CASP3 (9665), CASP7 (9492), CASP1 (24232), and GAPDH (2118) were purchased from Cell Signaling Technologies. TRAM antibody was purchased from Santa Cruz (sc-376076). FADD antibody (05–486) was purchased from Millipore Sigma. GSDMD antibody (ab209845). Secondary antibodies, anti-rabbit IgG (H+L) (DyLight^™ 800 4X PEG Conjugate) (5151) and anti-mouse IgG (H+L) (DyLight^™ 800 4X PEG Conjugate) (5257) were purchased from Cell Signaling Technologies.

Maxwell P.H., Dachs G.U., Gleadle J.M., Nicholls L.G., Harris A.L., Stratford I.J., Hankinson O., Pugh C.W, & Ratcliffe P.J. (1997). Hypoxia-inducible factor-1 modulates gene expression in solid tumors and influences both angiogenesis and tumor growth. Proceedings of the National Academy of Sciences of the United States of America, 94(15), 8104-8109.

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