Mir 1202

Manufactured by Qiagen

The MiR-1202 is a compact and automated liquid handling system designed for high-throughput processing of microRNA samples. It features precise pipetting, efficient sample preparation, and fast processing times to streamline microRNA analysis workflows.

Automatically generated - may contain errors

Lab products found in correlation

Quant it ribogreen rna assay kit, by Thermo Fisher Scientific (2 mentions) Mirneasy kit, by Qiagen (2 mentions) Mircury lna sybr green pcr kit, by Qiagen (2 mentions) Mircury lna universal rt microrna pcr kit, by Qiagen (2 mentions) Quantstudio 7 flex real time pcr system, by Thermo Fisher Scientific (2 mentions) Qiazol lysis buffer, by Qiagen (2 mentions)

2 protocols using mir 1202

Exosomal miRNA Profiling Protocol

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Exosomes were lysed using QIAzol lysis buffer (Qiagen). Total RNA was extracted from ∼1 to 2 × 10¹² exosomes using the miRNeasy kit (Qiagen) per the manufacturer's protocol and quantified using the Quant-iT RiboGreen RNA Assay kit (ThermoFisher). Before cDNA synthesis, synthetic RNA spike-in UniSp6 was added to each sample for normalization. Equal amounts of RNA from each sample were converted to cDNA using the miRCURY LNA Universal RT microRNA PCR kit (Qiagen). Quantitative real-time PCR was performed using the miRCURY LNA SYBR Green PCR Kit (Qiagen) on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Foster City, Calif). The expression of miR-21, miR-23b, miR-122, miR-126, miR-143, miR-145, miR-146a, miR-146b, miR-155, miR-181b, miR-195, miR-208a, miR-219, miR-221, miR-222, and miR-1202 (Qiagen; see Supplementary Table, for primer sequences) was normalized to RNA spike-in UniSp6.

Sorrentino T.A., Duong P., Bouchareychas L., Chen M., Chung A., Schaller M.S., Oskowitz A., Raffai R.L, & Conte M.S. (2020). Circulating exosomes from patients with peripheral artery disease influence vascular cell migration and contain distinct microRNA cargo. JVS-Vascular Science, 1, 28-41.

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Exosomal miRNA Profiling by qRT-PCR

Check if the same lab product or an alternative is used in the 5 most similar protocols

Exosomes were lysed using QIAzol lysis buffer (Qiagen). Total RNA was extracted from ∼1 to 2 × 10¹² exosomes using the miRNeasy kit (Qiagen) per the manufacturer’s protocol and quantified using the Quant-iT RiboGreen RNA Assay kit (ThermoFisher). Before cDNA synthesis, synthetic RNA spike-in UniSp6 was added to each sample for normalization. Equal amounts of RNA from each sample were converted to cDNA using the miRCURY LNA Universal RT microRNA PCR kit (Qiagen). Quantitative real-time PCR was performed using the miRCURY LNA SYBR Green PCR Kit (Qiagen) on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Foster City, Calif). The expression of miR-21, miR-23b, miR-122, miR-126, miR-143, miR-145, miR-146a, miR-146b, miR-155, miR-181b, miR-195, miR-208a, miR-219, miR-221, miR222, and miR-1202 (Qiagen; see Supplementary Table, for primer sequences) was normalized to RNA spike-in UniSp6.

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