After an overnight fast (≥ 12 h fasting period), blood samples were collected by a physician between 08:00 and 11:00 a.m. Blood samples were taken by venipuncture from an arm vein using multifly needles (Sarstedt, Nümbrecht, Germany) into serum or EDTA monovettes (Sarstedt). All samples were stored at ~ 5 °C and shipped to external laboratories on the same day. Blood pressure was measured by the physician in the sitting position after a resting period of 3–5 min on both upper arms above the elbow.
Multifly needles
Manufactured by Sarstedt
Sourced in Germany
Multifly needles are disposable blood collection devices designed for venous blood sampling. They feature a multi-lumen design to enable simultaneous collection of multiple blood samples with a single needle insertion.
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Lab products found in correlation
2 protocols using multifly needles
1
Fasted Blood Sampling and Blood Pressure
2
Fasting Blood Analysis Protocol
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During each examination, fasting blood was collected, blood pressure was measured and subjects completed a questionnaire to obtain information about changes in medication and lifestyle habits (e.g. physical activity), as well as the tolerability of linseed and sunflower oil. Blood samples were obtained by venipuncture of an arm vein using Multiflyneedles (Sarstedt, Nümbrecht, Germany) into serum and EDTA monovettes (Sarstedt). All six examinations, including blood sampling, were performed at the same time for each subject. For analysis of fatty acids in RBCs, the cell sediment after centrifugation for 10 min at 1500g and 4 °C and removal of plasma was reconstituted in PBS to the initial blood volume, transferred into 1.5 mL Eppendorf tubes and immediately frozen and stored at -80 °C until extraction and analysis. All transfer steps were carried out on ice. Serum lipid levels, liver enzymes and small blood picture were determined in the LADR laboratory (Laborärztliche Arbeitsgemeinschaft für Diagnostik und Rationalisierung e.V.), Hannover, Germany.
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