Fvb nhsd males

Manufactured by Inotiv

FvB/NHsD males are a strain of laboratory mice. They are a widely used model organism in biomedical research.

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Lab products found in correlation

C57bl 6j, by Charles River Laboratories (4 mentions) Sealsafe 1145t, by Tecniplast (4 mentions) Fvb nhsd, by Inotiv (2 mentions)

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FvB/NHsD

4 protocols using fvb nhsd males

FvB/NHsD and C57Bl6/J Mouse Breeding

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For the neuronal cultures, FvB/NHsD females were crossed with FvB/NHsD males (both ordered at 8-10 weeks old from Envigo). For the in utero electroporation female FvB/NHsD (Envigo) were crossed with male C57Bl6/J (ordered at 8-10 weeks old from Charles River). All mice were kept group-housed in IVC cages (Sealsafe 1145T, Tecniplast) with bedding material (Lignocel BK 8/15 from Rettenmayer) on a 12/12 h light/dark cycle in 21°C (±1°C), humidity at 40-70% and with food pellets (801727CRM(P) from Special Dietary Service) and water available ad libitum. All animal experiments were approved by the Local Animal Experimentation Ethical Committee, in accordance with Institutional Animal Care and Use Committee guidelines.

Proietti Onori M., Koopal B., Everman D.B., Worthington J.D., Jones J.R., Ploeg M.A., Mientjes E., van Bon B.W., Kleefstra T., Schulman H., Kushner S.A., Küry S., Elgersma Y, & van Woerden G.M. (2018). The intellectual disability‐associated CAMK2G p.Arg292Pro mutation acts as a pathogenic gain‐of‐function. Human Mutation, 39(12), 2008-2024.

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Murine Neuronal Culture and In Utero Electroporation

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For the neuronal cultures, FvB/NHsD females were crossed with FvB/NHsD males (both ordered at 8–10 weeks old from Envigo). For the in utero electroporation, female FvB/NHsD (Envigo) were crossed with male C57Bl6/J (ordered at 8–10 weeks old from Charles River). All mice were kept group‐housed in IVC cages (Sealsafe 1145T, Tecniplast) with bedding material (Lignocel BK 8/15 from Rettenmayer) on a 12/12 h light/dark cycle in 21°C (±1°C), humidity at 40–70% and with food pellets (801727CRM(P) from Special Dietary Service) and water available ad libitum. All animal experiments were approved by the Local Animal Experimentation Ethical Committee, in accordance with Institutional Animal Care and Use Committee guidelines.

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Mouse Breeding and Housing for Neuronal Cultures

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FvB/NHsD females were crossed with FvB/NHsD males (ordered at 8–10 weeks old from Envigo) for the neuronal cultures, whereas for the in utero electroporation female, FvB/NHsD (Envigo) were crossed with male C57Bl6/J (ordered at 8–10 weeks old from Charles River). All mice were kept group-housed in IVC cages (Sealsafe 1145T, Tecniplast) with bedding material (Lignocel BK 8/15 from Rettenmayer) on a 12/12 h light/dark cycle in 21 °C (±1 °C), humidity at 40–70%. Food pellets (801727CRM(P) from Special Dietary Service) and water were available ad libitum. All animal experiments were conducted in accordance with the European Commission Council Directive 2010/63/EU (CCD approval AVD101002017893).

Pellikaan K., van Woerden G.M., Kleinendorst L., Rosenberg A.G., Horsthemke B., Grosser C., van Zutven L.J., van Rossum E.F., van der Lely A.J., Resnick J.L., Brüggenwirth H.T., van Haelst M.M, & de Graaff L.C. (2021). The Diagnostic Journey of a Patient with Prader–Willi-Like Syndrome and a Unique Homozygous SNURF-SNRPN Variant; Bio-Molecular Analysis and Review of the Literature. Genes, 12(6), 875.

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Neuronal Culture Generation and In Utero Electroporation

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For the neuronal cultures, FvB/NHsD females were crossed with FvB/NHsD males (both ordered at 8‐ to 10‐weeks old from Envigo). For the in utero electroporation, female FvB/NHsD (Envigo) were crossed with male C57Bl6/J (ordered at 8‐to 10‐weeks old from Charles River). All mice were kept group‐housed in IVC cages (Sealsafe 1145T; Tecniplast) with bedding material (Lignocel BK 8/15 from Rettenmayer) on a 12‐h light/dark cycle at 21°C ± 1°C, humidity at 40%–70% and with food pellets (801727CRM(P) from Special Dietary Service) and water available ad libitum. All animal experiments were conducted in accordance with the European Commission Council Directive 2010/63/EU (CCD approval AVD101002017893).

van Woerden G.M., Bos M., de Konink C., Distel B., Avagliano Trezza R., Shur N.E., Barañano K., Mahida S., Chassevent A., Schreiber A., Erwin A.L., Gripp K.W., Rehman F., Brulleman S., McCormack R., de Geus G., Kalsner L., Sorlin A., Bruel A., Koolen D.A., Gabriel M.K., Rossi M., Fitzpatrick D.R., Wilkie A.O., Calpena E., Johnson D., Brooks A., van Slegtenhorst M., Fleischer J., Groepper D., Lindstrom K., Innes A.M., Goodwin A., Humberson J., Noyes A., Langley K.G., Telegrafi A., Blevins A., Hoffman J., Guillen Sacoto M.J., Juusola J., Monaghan K.G., Punj S., Simon M., Pfundt R., Elgersma Y, & Kleefstra T. (2021). TAOK1 is associated with neurodevelopmental disorder and essential for neuronal maturation and cortical development. Human Mutation, 42(4), 445-459.

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