Alpha platinum atr fourier transform spectrometer

TLC was carried out using Merck silica gel coated aluminium sheets as the stationary phase (Merck Kieselgel 60F₂₅₄ 230–400 mesh). The TLC plate was visualised using a UV lamp (254 nm) and stained using potassium permanganate solution. ¹H and ¹³C NMR were performed on a Bruker DPX‐400 spectrometer, at 400 MHz and 100 MHz, respectively. All chemical shifts were in parts per million (ppm) relative to the tetramethylsilane (TMS) internal standard (0.03 % v/v, 0.00 ppm). Coupling constants (J) were expressed in Hertz (Hz). ¹H NMRs were obtained at room temperature and using deuterated chloroform as solvent. The progress of epoxidation of cocoa butter was monitored by disappearance of double bond (δ = 5.30–5.39 ppm) and appearance of oxirane bond (δ = 2.85–2.96 ppm). Glycerol bond signal at 4.25–4.34 ppm was used as an internal standard, according to methodology described elsewhere.48 Infrared spectra were recorded on a Bruker ALPHA platinum ATR Fourier transform spectrometer. Absorptions were recorded in wavenumbers (cm⁻¹). Mass spectrometry was achieved using an Agilent 6130B single Quad (ESI).

¹H and ¹³C NMR were performed on a Bruker DPX‐400 spectrometer, at 400 MHz and 100 MHz, respectively. All chemical shifts were in parts per million (ppm) relative to the tetramethylsilane (TMS) internal standard (0.03% v/v, 0.00 ppm). Coupling constants (J) were expressed in Hertz (Hz). Infrared spectra were recorded on a Bruker ALPHA platinum ATR Fourier transform spectrometer. Absorptions were recorded in wavenumbers (cm⁻¹). Mass spectrometry was achieved using an Agilent 6130B single Quad (ESI). GPC was performed on an Agilent 390‐MDS with autosampler using a PL gel 5.0 µm bead‐size guard column (50 × 7.5 mm), followed by two linear 5.0 µm bead‐size PL gel Mixed D columns (300 × 7.5 mm) and a differential refractive index detector. Using CHCl₃ as eluent the system was calibrated using linear poly(styrene) Easi Vial standards (Agilent Ltd) range from 162 to 5×10⁵ Da. Data were collected and analysed using Cirrus GPC/SEC (v. 3.3) and Agilent GPC/SEC software. TGA, DSC and melting points were carried out using Metler Toledo DSC1‐Star with 40 µL standard aluminium pans and autosampler. Samples heated from −100–70°C, cooled to −100 and heated from −100–600°C under N₂ atmosphere for DSC and heated from 25–600°C for TGA.