Tube formation assay was performed as previously described [25 (link)]. Briefly, ECs (2 × 104 cells/well) were seeded into a 96-well plate pre-coated with 40 μL reduced-growth factor ECM gel (Life Technologies, NY, USA) and incubated in a regular incubator or in an air-tight chamber (MIC-101, Billups-Rothenberg, Inc., CA, USA) flushed with 95% N2/5% CO2, at 37 °C for 6 hours. Photos of tube-like structure were captured with an AMG inverted phase-contrast microscope (AMG, Life Technologies, NY, USA). The numbers of branching points and segments were quantified by NIH Image J software with angiogenesis analyzer plug-in (developed by Gilles Carpentier, http://image.bio.methods.free.fr/ImageJ/?Angiogenesis-Analyzer-for-ImageJ ).
Reduced growth factor ecm gel
Manufactured by Thermo Fisher Scientific
Sourced in United States
Reduced-growth factor ECM gel is a type of extracellular matrix (ECM) substrate designed for cell culture applications. It provides a more defined and simplified ECM environment compared to traditional ECM gels. The core function of this product is to support the attachment, growth, and differentiation of various cell types in vitro.
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Lab products found in correlation
2 protocols using reduced growth factor ecm gel
1
Tube Formation Assay for Angiogenesis Analysis
2
Angiogenesis Assay for PAECs
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PAECs (1×104 cells/well) were seeded into a 96‐well plate precoated with 40 μL reduced‐growth factor ECM gel (Life Technologies, New York, NY) and incubated for 6 hours. A tubelike structure was captured with an inverted phase‐contrast microscope (AMG, Life Technologies). The numbers of branching points and segments were quantified by Image J software with angiogenesis analyzer plug‐in (developed by Gilles Carpentier).
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