Blood was obtained by cardiac puncture. Plasma was prepared by centrifugation at 3000 rpm for 10 min. urea, creatinine and fT4 was measured in the central laboratory medicine unit of the University Hospital of Cologne using the kinetic UV test (urea, Roche Diagnostics), creatinine plus test vers.2 (creatinine, Roche Diagnostics) and fT4 II test (fT4, Roche Diagnostics). Significance was calculated using a two-tailed Student’s t test for all measurements.
Urea
Manufactured by Roche
Sourced in United States, Germany, United Kingdom
Urea is a chemical compound that is commonly used in laboratory settings. It is a colorless, odorless, and highly soluble crystalline solid. The core function of urea is as a source of nitrogen in various biochemical processes and analyses.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Thermo Fisher Scientific (3 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Ammonium bicarbonate, by Roche (1 mentions)
Tryple express enzyme, by Thermo Fisher Scientific (1 mentions)
L dopa, by Merck Group (1 mentions)
Complete protease inhibitor cocktail, by Roche (1 mentions)
Sodium fluoride, by Roche (1 mentions)
Trifluoroacetic acid, by Thermo Fisher Scientific (1 mentions)
Trypsin protease, by Promega (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Roche (1 mentions)
Lysyl endopeptidase lys c, by Fujifilm (1 mentions)
Tris hcl, by Thermo Fisher Scientific (1 mentions)
Ammonium formate, by Merck Group (1 mentions)
Iodoacetamide, by Merck Group (1 mentions)
Phosphatase inhibitor cocktail 2, by Merck Group (1 mentions)
Phosphatase inhibitor cocktail 3, by Merck Group (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Roche (1 mentions)
Leupeptin, by Roche (1 mentions)
Dithiothreitol (dtt), by Roche (1 mentions)
Phenylmethanesulfonyl fluoride, by Merck Group (1 mentions)
5 protocols using urea
1
Plasma Biomarkers in Animal Model
2
Melanogenesis Inhibition Assay with L-DOPA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following reagents were sourced from Sigma-Aldrich (Saint Louis, MI, USA): L-DOPA (Cat #D9628); urea (Cat #U5378); thiourea (Cat #T7875); C7BzO (Cat #C0856); Dulbecco’s Modified Eagle Medium (DMEM; Cat#D6429) (New York, NY, USA); cOmplete™ Protease Inhibitor Cocktail (Cat #11697498001) (Roche, USA); tris(2-carboxyethyl)phosphine (TCEP; Cat #C4706); ammonium bicarbonate (Cat #A6141); Empore™ Extraction Disk Cartridge C18 (Cat #66873-U) (Supelco, Bellefonte, PA, USA); acetonitrile hypergrade for LC–MS LiChrosolv® (Cat #1.00029) (Truganina, Australia); triflouroacetic acid (Cat #T6508); tyrosinase (Cat #T3824); boric acid (Cat #1.00165); sodium hyrdoxide (Cat # 221465); ascorbic acid (Cat # A4544); hydroxylamine solution (Cat # 467804). The following reagents were sourced from Thermo Fisher: TrypLE™ Express Enzyme (Cat #12604013) (Gibco, New York, NY, USA); Pierce™ BCA Protein Assay Kit (Cat #23225) (Thermo Scientific, Rockford, IL, USA). The UCP-5 peptide was synthesised by the Payne Laboratory with sequence: EEGVLALYSGIAPALLR.
3
Mass Spectrometry Sample Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Urea, sodium chloride, EDTA, bovine aprotinin, leupeptin (Roche), PMSF, sodium fluoride, Phosphatase Inhibitor Cocktail 2, and Phosphatase Inhibitor Cocktail 3, O-(2-acetamido-2-deoxy-d-glucopyranosylidene)amino-N-phenylcarbamate, iodoacetamide, and ammonium formate were purchased from Sigma–Aldrich. Tris–HCl, 1,4-DTT, and all MS-grade solvents, including water, formic acid, trifluoroacetic acid, and acetonitrile (ACN), were purchased from Thermo Fisher Scientific. Lysyl endopeptidase (Lys-C) was purchased from Wako Chemicals, and trypsin protease was purchased from Promega.
4
Tissue Lysis and Protein Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LV samples (50 mg) were obtained then immediately snap-frozen and stored in liquid nitrogen. The samples were pulverized under liquid nitrogen into a fine powder, which was homogenized in a lysate buffer containing 8 mol/l urea, 1 mol/l dithiothreitol (13 (link)) (Roche Diagnostics GmbH, Mannheim, Germany), leupeptinand, aprotinin, pepstatin (all 1 mg/ml), radioimmunoprecipitation assay buffer and 0.1% phenylmethanesulfonyl fluoride (w/v; Sigma-Aldrich, St. Louis, MO, USA) and then lysed on ice for 30 min. The samples were lysed further by sonication for 2 min. The total lysate was centrifuged for 15 min at 4°C and 10,000–14,000 × g, and the final supernatant was collected.
5
Urine Composition Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Urine was collected over three 12-hour periods with participants encouraged to void before entering the room. Collected urine volume was measured using digital weighing scales (Salter 323, Salter, UK) and analysed enzymatically for UREA and creatinine (UREA and CREAT kits, respectively from Roche, DE) using an automated clinical chemistry analyser (Cobas c702, Roche, DE).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!