We measured vapor-phase nicotine using a passive diffusion-based sampler containing a filter treated with sodium bisulfate assembled in our laboratory (Jones et al., 2012 ). Filters were extracted with an internal standard (isoquinoline, Sigma-Aldrich, St. Louis, MO, USA) and analyzed using a gas chromatograph with a nitrogen phosphorus detector (GC-FTD, Shimadzu GC-2014, Shimadzu, Columbia, MD, USA). Nicotine was separated using a capillary column (SHRXI-5MS, Shimadzu, Columbia, MD, USA). We collected 10% field blanks and duplicates. Nicotine concentrations in duplicate monitors were similar and were averaged together. The 17% of nicotine values below the LOD of 0.021 μg/mL (equivalent to 0.049 μg/m3 for a 72 hour duration) were replaced with half the batch-specific LOD.
Shrxi 5ms
Manufactured by Shimadzu
Sourced in United States, Japan
The SHRXI-5MS is a high-performance gas chromatography-mass spectrometry (GC-MS) system designed for precise and accurate analysis. It features a high-sensitivity quadrupole mass spectrometer and advanced data processing software to provide reliable results. The core function of the SHRXI-5MS is to enable efficient separation, identification, and quantification of a wide range of chemical compounds.
Automatically generated - may contain errors
Lab products found in correlation
Gc 2014, by Shimadzu (2 mentions)
Isoquinoline, by Merck Group (1 mentions)
Aoc 20iplus, by Shimadzu (1 mentions)
Gc 2010 plus, by Shimadzu (1 mentions)
Gcms qp2010 ultra gas chromatograph, by Shimadzu (1 mentions)
Qp 2010 plus system, by Shimadzu (1 mentions)
Qp2010 plus gc ms, by Shimadzu (1 mentions)
7 protocols using shrxi 5ms
1
Vapor-Phase Nicotine Measurement Protocol
2
GC-MS Analysis of Essential Oils
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The essential oil was analyzed by gas chromatography coupled to mass spectrometry detection (GC-MS) using a GC-2010 Plus (Shimadzu, Kyoto, Japan) system equipped with a AOC-20iPlus (Shimadzu, Kyoto, Japan) automatic injector and a SH-RXi-5ms (30 m × 0.25 mm × 0.25 μm; Shimadzu, Kyoto, Japan, USA) column. The conditions regarding the temperature set for the injector, oven, transfer line, and ion source temperatures were the same as previously described by Spréa et al. (2020) [9 (link)]. The volume of the injected sample was 1 μL with a split ratio of 1:10. Helium was used as the carrier gas, adjusted to a linear velocity of 30 cm/s. The ionization energy was 70 eV, scan range was 35–500 u, with a scan time of 0.3 s. The compounds’ identification was based on a comparison of the obtained mass spectra with those of the Nacional Institute of Standards and Technology (NIST2017) mass spectra library and linear retention index (LRI), calculated using the retention times of an n-alkane series (C8-C40, ref. 40147-U, Supelco), and analyzed under identical conditions. The calculated LRI was compared with previously published data [10 ]. Compounds were quantified as a relative percentage of total volatiles using the relative area values directly obtained from peak total ion current (TIC). Analyses were performed in triplicate.
3
Volatile Compound Analysis of Samples
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The volatile constituents of each sample were analyzed using a Shimadzu GCMS‐QP2010 Ultra gas chromatograph with mass selective detector (Shimadzu) equipped with GCMSsolutions 2.53 and capillary nonpolar column (SHRXI‐5MS, Shimadzu, 30 m * 0.25 mm id * 0.25 μm film thickness). The oven temperature was initially held at 50°C for 5 min and then increased at 4º C/min to final temperature of 250°C. The injector temperature was 200°C and injections were made in splitless mode. Ultra high purity helium used as a carrier gas at a flow rate of 0.69 mL/min (approximately 25 cm/sec linear flow velocity). The mass spectra were collected at m/z 40–400 and were performed every 0.3 sec. The ion source and quadrupole were set at 230 and 200°C respectively. Identification of the volatile components was performed by combined matching standardized retention time (LRI values) for a DB‐5 column (Flavornet and Pherobase) and fragmentation spectra of standards from NIST 11 (Scientific Instrument Services, Ringoes, NJ) and the Wiley 2010 libraries (John Wiley and Sons Inc.). Confirmation of the identification was sought by matching the mass spectra of the compounds with the reference mass spectra present in the NIST 11 and Wiley libraries. The results were compared with our control, unpanned samples.
4
Catalytic Transfer Hydrogenolysis of 5-HMF to 2,5-DMF
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The catalytic transfer hydrogenolysis reaction of 5-HMF to 2,5-DMF was carried out using the Biotage Reactor (Biotage Endeavor Catalyst Screening System) under programmed experimental conditions. A 4 mL of 20 mM of 5-HMF in i-propanol with 10 mg of the catalyst was charged into a glass liner reactor. After the reaction, the eluent was filtered and submitted to a Gas Chromatograph (GC-2014, Shimadzu, Japan) equipped with a flame ionization detector (FID) and a 30 m capillary column (SH-RXI 5MS, Shimadzu) for the quantification of unreacted feed and desired products. The vaporization of the aliquot (1 μL) of resulting solutions has occurred at 200 °C up on injection and the separation was programmed as: (a) ramping up (40 °C/min) from the initial temperature of 40 °C–115 °C after 5 min of equilibration, followed by (b) ramping up (20 °C/min) from 115 °C to 150 °C after 2 min of equilibration. Carried by Helium gas with a steady flow of 1.3 mL min-1, the sample would reach the FID detector for complete combustion at 250 °C. After quantitively quantifying the products by using GC-FID, the conversion of HMF and the product yield were calculated by Ref. [59 (link)]:
5
GC-MS Analysis of Alkaloid Fractions
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Each alkaloidal fraction was analyzed by GC-MS on a Shimadzu QP2010 Plus system (Shimadzu, Columbia, MD, USA), using a fused capillary silica column Shimadzu SHRXi 5MS (30 m × 0.25 mm; 0.25 μm coating thickness). The injection port, ionization chamber, and transfer line temperatures were 250, 230, and 325 °C, respectively. The separation was conducted by running a temperature program as follows: 70 °C (2 min), 10 °C/min up to 310 °C, and finally, the temperature was held (10 min). A quadrupole analyzer in automatic frequency scanning (full scan) was used over the mass range m/z 40–400.
Mass spectra were obtained by electron ionization (EI) at 70 eV. The alkaloidal fractions were properly dissolved in chloroform before analysis and 1 μL of such solutions was injected using a split ratio of 1:10. Helium 4.5 was used as a carrier gas at a 1 mL/min flow rate. The components of each fraction were identified by comparison of their MS spectra with those reported in the database NIST95 and the literature [27 (link),29 ]. Retention indexes (RI) were also calculated from a homologous series of n-alkanes (C12–C28) to support the identification [27 (link)].
Mass spectra were obtained by electron ionization (EI) at 70 eV. The alkaloidal fractions were properly dissolved in chloroform before analysis and 1 μL of such solutions was injected using a split ratio of 1:10. Helium 4.5 was used as a carrier gas at a 1 mL/min flow rate. The components of each fraction were identified by comparison of their MS spectra with those reported in the database NIST95 and the literature [27 (link),29 ]. Retention indexes (RI) were also calculated from a homologous series of n-alkanes (C12–C28) to support the identification [27 (link)].
6
Adsorption of Octamethylcyclotetrasiloxane using [Cu(CA)2]
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The [Cu(CA)2] was
subjected to an adsorption test for the removal of octamethylcyclotetrasiloxane
(D4) from gaseous streams. The adsorption experiment was carried out
using a mass/volume ratio of 1 g/L, D4 initial concentration of 400
mg/Nm3, 25 °C, 1 atm. First, a volume of D4 (analytical
grade, 98% of purity, provided by Sigma-Aldrich) was volatilized into
a 1.48 L glass flask to obtain a D4 gaseous solution. Then, the mixture
was diluted to obtain a concentration of 400 mg/Nm3. This
solution was put into contact with 100 mg of [Cu(CA)2]
during 24 h until the equilibrium was reached. Several aliquots were
taken and analyzed by a Nexis GC 2030 gas chromatograph with a flame
ionization detector to monitor the siloxane concentration. The column
used was an SH-Rxi-5ms SHIMADZU (15.0 m × 0.25 mm inner diameter
× 0.25 μm film thickness). The temperature of the injector
port, oven, and detector were set at 150, 110, and 250 °C, respectively.
Helium was used as the carrier gas at a constant flow rate of 1.50
mL/min and pressure of 12.08 psi. Gas samples of 500 μL were
injected to the GC. The duration of the method was 1.4 min. Finally,
the uptake capacity was obtained by mass balance followingeq 1 .
subjected to an adsorption test for the removal of octamethylcyclotetrasiloxane
(D4) from gaseous streams. The adsorption experiment was carried out
using a mass/volume ratio of 1 g/L, D4 initial concentration of 400
mg/Nm3, 25 °C, 1 atm. First, a volume of D4 (analytical
grade, 98% of purity, provided by Sigma-Aldrich) was volatilized into
a 1.48 L glass flask to obtain a D4 gaseous solution. Then, the mixture
was diluted to obtain a concentration of 400 mg/Nm3. This
solution was put into contact with 100 mg of [Cu(CA)2]
during 24 h until the equilibrium was reached. Several aliquots were
taken and analyzed by a Nexis GC 2030 gas chromatograph with a flame
ionization detector to monitor the siloxane concentration. The column
used was an SH-Rxi-5ms SHIMADZU (15.0 m × 0.25 mm inner diameter
× 0.25 μm film thickness). The temperature of the injector
port, oven, and detector were set at 150, 110, and 250 °C, respectively.
Helium was used as the carrier gas at a constant flow rate of 1.50
mL/min and pressure of 12.08 psi. Gas samples of 500 μL were
injected to the GC. The duration of the method was 1.4 min. Finally,
the uptake capacity was obtained by mass balance following
7
GC-MS Analysis of Fecal Short-Chain Fatty Acids
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